排序方式: 共有12条查询结果,搜索用时 31 毫秒
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Maryam Farshadi Behrooz Johari Elham Erfani Ezadyar Mazaher Gholipourmalekabadi Mahmoud Azami Hamid Madanchi Seyed Mohammad Amin Haramshahi Abazar Yari Ayoob Karimizade Reza Nekouian Ali Samadikuchaksaraei 《Cell biology international》2019,43(12):1379-1392
The mechanical property of bone tissue scaffolds is one of the most important aspects in bone tissue engineering that has remained problematic. In our previous study, we fabricated a three‐dimensional scaffold from nano‐hydroxyapatite/gelatin (nHA/Gel) and investigated its efficiency in promoting bone regeneration both in vitro and in vivo. In the present study, the effect of adding silicon carbide (SiC) on the mechanical and biological behaviors of the nHA/Gel/SiC and bone regeneration in vivo were determined. nHA and SiC were synthesized and characterized by the X‐ray diffraction pattern and transmission electron microscope image. Layer solvent casting, freeze drying, and lamination techniques were applied to prepare these scaffolds. Then, the biocompatibility and cell adhesion behavior of the synthesized nHA/Gel/SiC scaffolds were investigated. For in vivo studies, rats were categorized into three groups: blank defect, blank scaffold, and rat bone marrow mesenchymal stem cells (rBM‐MSCs)/scaffold. After 1, 4, and 12 weeks post‐injury, the rats were sacrificed and the calvaria were harvested. Sections with a thickness of 5 µm thickness were prepared and stained with hematoxylin–eosin and Masson's Trichrome, and immunohistochemistry was performed. Our results showed that SiC effectively increased the mechanical properties of the nHA/Gel/SiC scaffold. No significant differences were observed in biocompatibility, cell adhesion, and cytotoxicity of the nHA/Gel/SiC in comparison with the nHA/Gel nanocomposite. Based on histological and immunohistochemical studies, both osteogenesis and collagenization were significantly higher in the rBM‐MSCs/scaffold group, quantitatively and qualitatively. The present study strongly suggests the potential of SiC as an alternative strategy to improve the mechanical and biological properties of bone tissue engineering scaffolds, and shows that the pre‐seeded nHA/Gel/SiC scaffold with rBM‐MSCs improves osteogenesis in the engineered bone implant. 相似文献
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Abazar Yari Mohammad Hossein Asadi Hossein Bahadoran Hossein Dashtnavard Hossein Imani Mohammad Reza Naghii 《Biological trace element research》2010,137(2):216-225
In this study, the effects of cadmium toxicity and the protective effects of l-carnitine on spermatogenesis in Sprague–Dawley rat were evaluated. Animals were subdivided into five groups. Cadmium chloride
(1-mg/kg body weight) was injected intraperitoneally during 16 days at intervals of 48 h between subsequent treatments. l-Carnitine (500 mg/kg b.w., IP) was pretreated in both of control and cadmium-injected rats. Animals were killed on day 17
after the first treatment. The left cauda epididymis was removed and immediately immersed into Hank’s balanced salt solution
for evaluation of sperm count and viability. Following contamination with cadmium, a decrease in the number and viability
of cauda epididymis sperm, the number of cell proliferation, and Johnsen Scores in the seminiferous tubules was observed.
Consequently, l-carnitine treatment caused an increase in the number and viability of cauda epididymis sperm, the number of cell proliferation,
and Johnsen Scores in the cadmium-induced group. 相似文献
4.
Safari M Ghanati F Hajnoruzi A Rezaei A Abdolmaleki P Mokhtari-Dizaji M 《Biotechnology letters》2012,34(6):1137-1141
Suspension-cultured hazel cells were ultrasonicated at power densities of 4 and 455 mW for 4–40 min. Both treatments stimulated
the production of major taxanes: Taxol, 10-deacetylbaccatin, and baccatin III. The highest amounts of these taxanes (0.46,
0.26, and 0.07 mg/l, respectively) were obtained at 8 and 20 min of the treatment at 455 mW. Ultrasound had no adverse effects
on cell viability, growth, or membrane integrity. Increased release of taxanes by ultrasound resulted not from increased membrane
permeability but more likely from stimulation of taxanes biosynthesis. 相似文献
5.
Ghasemi-Omran Vali Ollah Ghorbani Abazar Sajjadi-Otaghsara Seyyed Abdolkarim 《In vitro cellular & developmental biology. Plant》2021,57(2):319-331
In Vitro Cellular & Developmental Biology - Plant - Melatonin has been shown to play a variety of roles in regulating plant growth and response to various abiotic stresses, but its main... 相似文献
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Ghorbani Abazar Pishkar Leila Roodbari Nasim Tavakoli Sara Ali Jahromi Elham Moein Wu Chu 《Journal of Plant Growth Regulation》2023,42(2):1107-1119
Journal of Plant Growth Regulation - The study was conducted to consider the role of nitrate reductase (NR)-synthesized nitric oxide (NO) in the methyl jasmonate (MJ)-induced tolerance of arsenic... 相似文献
7.
Masoumeh Safari Faezeh Ghanati Mehrdad Behmanesh Abazar Hajnorouzi Bahareh Nahidian Ghahremani Mina 《Acta Physiologiae Plantarum》2013,35(9):2847-2855
Induction of antioxidant systems of hazel cells by low-energy ultrasound, the potential role of hydrogen peroxide (H2O2) as a signaling molecule in regulation of activity of stress-related enzymes, and expression of catalase (CAT) and phenylalanine ammonialyase (PAL) genes were investigated. Suspension-cultured Corylus avellana L. cells were agitated by an ultrasonic device at 29 kHz with the power of 4 mW/cm2, for 8–40 min. The activities of CAT, superoxide dismutase (SOD), and ascorbate peroxidase (APX) of treated cells increased by 4, 1.7 and 7 times of the control ones, respectively. Induction of increase in the expression of CAT gene started 24 h after the treatment with ultrasound. Significant increase also was observed in the expression of PAL gene, 6 h after exposure to ultrasound, which resulted in turn to increase of total contents of soluble phenolics, 24 h of the treatment. Exposure to ultrasound up to 20 min had no adverse effects on cell viability although it slightly increased the accumulation of H2O2. However, it is likely that this level of increased H2O2 was not deteriorative for hazel cells, but rather triggered antioxidant system and provided hazel cells a sustainable growth after ultrasound treatment. 相似文献
8.
GPx1 is one of the most important enzymes involved in oxidative balance so that, we studied the phenotype and genotype relationship of GPx1 activity and rs 1800668 (C/T) site and also evaluated the changes of GPx1 kinetic parameters in the rs 1800668 homozygotes. One hundred fifty eight subjects were recruited after clinical exams. The rs 1800668 (C/T) genotype distribution was identified using RFLP-PCR method. The hemolysate GPx1 activity was spectrophotometrically measured in a reaction coupled with glutathione reductase (GR). The GPx1 enzyme was purified using gel filtration chromatography with Sephacryl S-300 column and, Km(app) was studied in the rs 1800668 TT and CC homozygotes. The results showed that the GPx1 activity is significantly associated to the rs 1800668 (C/T) genotype distribution (P<0.05) so that, the GPx1 activity was high among the CC homozygotes (P<0.03). In addition, Km(app) for TBHP substrate in the TT homozygote (8.48 μM) was higher than the CC homozygote (5.74 μM). We concluded that the C allele within rs 1800668 position is related to the GPx1 activity and may be a potential factor involved in development of inflammatory events. 相似文献
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Carbon isotopes and water use efficiency: sense and sensitivity 总被引:1,自引:0,他引:1
We revisit the relationship between plant water use efficiency and carbon isotope signatures (δ13C) of plant material. Based on the definitions of intrinsic, instantaneous and integrated water use efficiency, we discuss
the implications for interpreting δ13C data from leaf to landscape levels, and across diurnal to decadal timescales. Previous studies have often applied a simplified,
linear relationship between δ13C, ratios of intercellular to ambient CO2 mole fraction (C
i/C
a), and water use efficiency. In contrast, photosynthetic 13C discrimination (Δ) is sensitive to the ratio of the chloroplast to ambient CO2 mole fraction, C
c/C
a (rather than C
i/C
a) and, consequently, to mesophyll conductance. Because mesophyll conductance may differ between species and over time, it
is not possible to determine C
c/C
a from the same gas exchange measurements as C
i/C
a. On the other hand, water use efficiency at the leaf level depends on evaporative demand, which does not directly affect
Δ. Water use efficiency and Δ can thus vary independently, making it difficult to obtain trends in water use efficiency from
δ13C data. As an alternative approach, we offer a model available at to explore how water use efficiency and 13C discrimination are related across leaf and canopy scales. The model provides a tool to investigate whether trends in Δ indicate
changes in leaf functional traits and/or environmental conditions during leaf growth, and how they are associated with trends
in plant water use efficiency. The model can be used, for example, to examine whether trends in δ13C signatures obtained from tree rings imply changes in tree water use efficiency in response to atmospheric CO2 increase. This is crucial for predicting how plants may respond to future climate change.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献