遗传标记与数量性状基因间连锁关系的分析

本文讨论标记基因与数量性状主基因连锁关系的一般分析方法,包括重组值的估计和有关遗传假设的测验。并以我们水稻遗传试验中两个具有互补和重叠作用的卷叶基因和一个矮秆基因试验结果的分析为例作了较详细的示范。     

九种罕见的人类染色体异常核型报告

ReportonNineRareSpeciesofHumanChromosomalAbnormalKaryotypesHanWeitian;QuOu;YuPing;JiangMiao(LiaoningResearchInstituteofFamilyPlanning,Shenyang110031)自1983年以来,我们对数千例不育及胚胎丢失等生殖异常患者进行细胞遗传学研究,发现大量异常染色体核型,而且异常种类繁多,已报道世界首报人类异常核型25种[1]。最近,在不良妊娠患者中又发现9种异常核型,经湖南医科大学医学细胞遗传学国家培训中心鉴定,为世界首次报道．现报告如下。1病例摘要及核型例1男,30岁,表型及智力正常,其妻子妊娠两次,均在无任何诱因情…     

基因工程抗体

基因工程抗体是８０年代发展起来的研究领域,把基因工程技术与单克隆抗体技术结合起来,创造出自然界不存在的各种抗体,如人－鼠嵌合抗体、改形抗体、单链抗体等,大大地降低或消除鼠源单克隆抗体的免疫原性,从而可以发挥抗体在肿瘤和病毒病治疗中的潜力。在此基础上发展起来的抗体库技术使人们有可能不经免疫获得任何一种抗体,将对医学和生物学的发展起着深远的影响。本文将对这两方面的内容作一简要的介绍。     

宋内氏痢疾菌侵袭相关基因的克隆与表达

用粘粒PJB8为载体,构建了宋内氏痢疾菌I相大质粒基因库。用菌落原位杂交,Western blot方法从文库中筛选到1株表达IpaA,B,C,D 4种蛋白的重组子。经重组质粒酶切和Southern blot等方法对宋内氏菌和福氏2a痢疾菌的侵袭相关基因片段作了初步比较分析。     

论受控于X连锁基因性状的变异性

本文应用数量遗传学方法,结合概率运算,从理论上分析了由X连锁基因控制的性状在雌雄群体中的变异情况。表明雄（男）性群体的这类性状遗传变异范围有大于雌（女）性群体的倾向。对这一问题的探讨,将有助于对X连锁基因及所决定性状的遗传特性、进化特点及基因定位等的研究提供新的思路。     

Potential inhibitors against 2019-nCoV coronavirus M protease from clinically approved medicines

Coronaviruses,members of the family Coronaviridae and sub-family Coronavirinae,are enveloped positive-strand RNA viruses which have spikes of glycoproteins projecting from their viral envelopes,thus exhibit a corona or halo-like appearance(Masters and Perlman,2013;Cui et al..2019).Coronaviruses are the causal path-ogens for a wide spectrum of respiratory and gastrointestinal diseases in both wild and domestic animals,including birds,pigs,rodents,etc(Dhama et al,2014).Previous studies have found that six strains of coronaviruses are capable to infect humans,including four strains circulating yearly to cause common cold,and other two strains which are the source for severe acute respiratory syndrome(SARS)and Middle East respiratory syndrome(MERS-CoV),respectively(Dhama et al,2014;Cui et al..2019).     

唇?硬骨蛋白基因克隆及其在肌间刺发生过程中的表达

为探究硬化蛋白(Sclerostin, SOST)与肌间刺发生之间的关系, 研究通过转录组测序和RT-PCR获得了唇?(Hemibarbus labeo)硬化蛋白基因cDNA序列。序列分析表明, 硬化蛋白由信号肽和成熟肽两部分组成, 成熟肽包含一个胱氨酸结样结构域。系统进化树分析表明, 唇?硬化蛋白与金鱼(Carassius auratus)硬化蛋白最为接近。通过荧光定量PCR检测发现唇?硬化蛋白基因mRNA在所有被检测的组织中均有表达, 在鳃中的表达量最高。RNA原位杂交结果表明, 硬化蛋白mRNA分布于肌膈, 且随着肌间刺的发生信号逐步减弱。RT-qPCR显示, 在肌间刺4个发育阶段中, 硬化蛋白基因mRNA的变化显著, 在阶段Ⅰ表达量最高, 随后逐步降低。综上, 唇?硬化蛋白与肌间刺骨化存在一定的相关性。研究结果将为进一步调查鱼类硬化蛋白功能及硬化蛋白在肌间刺形成过程中的作用提供理论依据。     

OSMRβ mutants enhance basal keratinocyte differentiation via inactivation of the STAT5/KLF7 axis in PLCA patients

Dear Editor, Primary localized cutaneous amyloidosis (PLCA) is a skin-limited disorder characterized by deposition of amyloid material in the superficial dermis.According to clinical characteristics,PLCA is divided into lichen,macular,and nodular amyloidosis.PLCA is found worldwide but has a higher incidence in South America and Southeast Asia,such as in Brazil and China (Chang et al.,2014;Tey et al.,2016).     

An Integrative Taxonomy of Amphibians of Nepal: An Updated Status and Distribution

Amphibians are declining worldwide.At the same time,each year many species of amphibians are described,indicating that biological diversity on Earth remains poorly understood.This is especially true for mountainous regions,such as the Himalayas,which are also regarded as a biodiversity hotspot.However,it is most likely that the current diversity of amphibians in Nepal is highly underestimated.Amphibian inventories by the past researchers primarily used phenotypic characters as the basis for species identification.However,the application of molecular systematics methods to taxonomic studies has successfully uncovered the taxonomic status of several cryptic taxa.This study used both molecular(16 S rDNA sequences)and morphological comparisons and estimated the diversity of amphibians from Nepal.This study reports the occurrence of 38 species of amphibians based on both molecular and morphological comparisons.Among them,three species were recorded for the first time in Nepal.This study also invalids the taxonomic status of Sphaerotheca paschima.This study highlights the importa nce of integrative taxonomic approaches that help to resolve the current taxonomic ambiguities of Nepalese amphibians.