Effect of various procedures of storing the fungus Rhizopus microsporus UzLT-1, a lipase producer

The effect of various storage procedures on the viability and synthesis of lipolytic enzymes by Rhizopus microsporus UzLT-1 was studied. The best procedures of producer storage proved to be regular passages and lyophilization. These procedures made it possible to maintain stable activity of lipolytic enzymes produced by the fungus for a long time. The fungal storage in vaseline oil or in a dried state was less effective due to significant losses of its lipolytic capacity.     

Synthesis,Fungistatic, Protistocidal,and Antibacterial Activity of 1-(3-Amino-2-Hydroxypropyl)Indoles

A series of new indole derivatives containing the 3-amino-2-hydroxypropyl group at the nitrogen atom has been synthesized by the ring-opening of the oxirane cycle of 1-oxiranylmethylindoles. Their antibacterial, fungicidal, and protistocidal activities have been studied. Most of the synthesized compounds have been shown to exhibit a high protistocidal activity that several times exceeds that of the reference drug, baikoks (toltrazuril).     

Synthesis of new 1-alkyl-, 1-benzyl-, and 1-aryloxyethyl-substituted 4,5-dichloroimidazoles and their antimicrobial,protistocidal, and fungistatic properties

Previously unknown 1-alkyl-, 1-benzyl-, and 1-aryloxyethylderivatives of dichloroimidazoles and products of their structural transformation were synthesized from 4,5-dichloroimidazole or 2-methyl-4,5- dichloroimidazole using alkyl, benzyl or aryloxyethyl halides. These N-substituted compounds were shown to have a weak antibacterial activity against some pathogenic gram-positive and gram-negative bacteria (Staphylococcus aureus and Escherichia coli). At the same time, some of the obtained compounds demonstrated a significant protistocidal activity against Colpoda steinii, which can exceed in strength the activity of clinically used veterinary drug Baycox. Moreover, these compounds showed a pronounced fungistatic effect.     

Predicted gene sequence C10orf112 is transcribed,exhibits tissue-specific expression,and may correspond to AD7

Case-control and prospective longitudinal studies have revealed an interaction of the anonymous D10S1423 234 bp allele with the APOE4 allele in determining the age-specific risk of Alzheimer's disease (AD). The D10S1423 polymorphism resides within intron 10 of open reading frame C10orf112, whose predicted product resembles a low-density lipoprotein receptor (NCBI Build 35.1). These observations suggest that the D10S1423 234 bp allele may be in linkage disequilibrium with a C10orf112 gene variant whose product interacts with the apoE4 lipoprotein. Our initial exploration of this hypothesis focused on validating the C10orf112 gene model. RT-PCR amplification from human hippocampal mRNA confirmed that 34 of the predicted 39 exons of C10orf112 were expressed in this brain region. Northern blots revealed 1.2 kb and 3.2 kb mRNA species that hybridize to a cDNA probe consisting of contiguous exons 23–26. Expression of these C10orf112 mRNA species was limited to a subset of brain regions and heart tissue.     

Recyclization of 9-bromocotarnine under the action of haloacylhetarenes. Synthesis and biological activity of the 4-heteroaroyl-9-bromo-1,2-dihydro-6-methoxy-7,8-methylenedioxy-3-benzazepines

Reaction of 9-bromocotarnine with heterocyclic α-halo ketones is accompanied by the expansion of the six-membered dihydropyridine ring to seven-membered dihydroazepine one and leads to previously unknown 4-heteroaroyl-9-bromo-3-methyl-1,2-dihydro-6-methoxy-7,8-methylenedioxy-3-benzazepines. It has been shown that some of the resulting compounds exhibit a significant antibacterial activity against Staphylococcus aureus and Escherichia coli. At the same time, the synthesized benzazepines have shown no significant protistocidal activity against Colpoda steinii and fungistatic activity against Penicillium italicum.     

Edelfosine-induced metabolic changes in cancer cells that precede the overproduction of reactive oxygen species and apoptosis

Background  

Metabolic flux profiling based on the analysis of distribution of stable isotope tracer in metabolites is an important method widely used in cancer research to understand the regulation of cell metabolism and elaborate new therapeutic strategies. Recently, we developed software Isodyn, which extends the methodology of kinetic modeling to the analysis of isotopic isomer distribution for the evaluation of cellular metabolic flux profile under relevant conditions. This tool can be applied to reveal the metabolic effect of proapoptotic drug edelfosine in leukemia Jurkat cell line, uncovering the mechanisms of induction of apoptosis in cancer cells.     

Synthesis and Anti-Infective Activity of 2-Heteroaryl(Acyl)-9,10,12,13-Tetramethoxy-3-Methyl-4,5-Dihydro-3<Emphasis Type="Italic">H</Emphasis>-Phenanthro[1,2-<Emphasis Type="Italic">d</Emphasis>]Azepines

2-Hetaryl- and 2-acyl-9,10,12,13-tetramethoxy-3-methyl-4,5-dihydro-3H-phenanthro[1,2-d]-azepines have been synthesized from 8,9,11,12-tetramethoxy-2-methyl-3,4-dihydronaphtho[2,1-f]isoquinolinium perchlorate by pyridine-azepine recyclization. The resulting compounds have revealed a pronounced antibacterial activity against Staphylococcus aureus (strain P-209) and Escherichia coli (field strain 078). Some of these compounds have a moderate fungistatic activity against Peniciliium italicum fungi. Two compounds have shown a certain activity in relation to the Colpoda steinii protozoa.     

Inheritance pattern of platelet membrane fluidity in Alzheimer disease.

The fluorescence anisotropy of 1,6-diphenyl-1,3,5-hexatriene in labeled platelet membranes, an index of membrane fluidity, is a stable, familial trait that is associated with a clinically distinct subtype of Alzheimer disease. Complex segregation analysis of this continuous variable was performed on 95 members of 14 pedigrees identified through probands who had autopsy-confirmed or clinically diagnosed Alzheimer disease. The results suggest that platelet membrane fluidity is controlled by a single genetic locus, PMF, with two alleles that have additive effects. The PMF locus appears to explain approximately 80% of the total variation in platelet membrane fluidity within the families of patients with Alzheimer disease.     

Mapping of the Proteinase B Structural Gene PRB1, in SACCHAROMYCES CEREVISIAE and Identification of Nonsense Alleles within the Locus

We report the mapping of the structural gene for proteinase B, PRB1. It is located 1.1 cM proximal to CAN1 on the left arm of chromosome V of Saccharomyces cerevisiae. We have identified 34 amber and 12 ochre mutations among the 126 prb1 mutations in our collection.