The development and use of an immunoenzyme test system for detecting and the species identification of the monkey pox virus]

An enzyme immunoassay (EIA) system for the species-specific diagnosis of monkeypox, based on the use of monoclonal antibodies (McAb) to monkeypox virus, has been developed. Immunoglobulins, isolated from McAb-containing cultural and immune ascitic fluids, have been conjugated with horse-radish peroxidase and used as detector antibodies. For immunosorption, rabbit polyclonal antibodies to the vaccine virus have been used. The specificity and sensitivity of the EIA system thus obtained have been tested on animals and humans having monkeypox and confirmed by traditional diagnostic methods (the isolation of the virus on chick embryo chorioallantoic membranes and in cell culture).     

Bacterial communities of some brown and red algae from Peter the Great Bay, the Sea of Japan

The structure of microbial communities of brown algae, red algae, and of the red alga Gracilaria verrucosa, healthy and affected with thallus rot, were comparatively investigated; 61 strains of heterotrophic bacteria were isolated and characterized. Most of them were identified to the genus level, some Vibrio spp., to the species level according to their phenotypic properties and the fatty acid composition of cellular lipids. The composition of the microflora of two species of brown algae was different. In Chordaria flagelliphormis, Pseudomonas spp. prevailed, and in Desmarestia viridis, Bacillus spp. The composition of the microflora of two red algae, G. verrucosa and Camphylaephora hyphaeoides, differed mainly in the ratio of prevailing groups of bacteria. The most abundant were bacteria of the CFB cluster and pseudoalteromonads. In addition, the following bacteria were found on the surface of the algae: Sulfitobacter spp., Halomonas spp., Acinetobacter sp., Planococcus sp., Arthrobacter sp., and Agromyces sp. From tissues of the affected G. verrucosa, only vibrios were isolated, both agarolytic and nonagarolytic. The existence of specific bacterial communities characteristic of different species of algae is suggested and the relation of Vibrio sp. to the pathological process in the tissues of G. verrucosa is supposed.     

Taxonomic composition of bacteria associated with cultivated mollusks <Emphasis Type="Italic">Crassostrea lugubris</Emphasis> and <Emphasis Type="Italic">Perna viridis</Emphasis> and with the water of the Gulf of Nha Trang lagoon,Vietnam

One hundred and four strains of heterotrophic bacteria have been isolated and characterized from two species of bivalve mollusks cultivated in the Gulf of Nha Trang (Vietnam) and from the water of a mariculture farm. The isolates have been identified on the basis of morphological, physiological, biochemical, and chemotaxonomic properties, as well as by the content of G+C bases in DNA. In the microflora of mollusks, Vibrio alginolyticus was predominant; the pathogenic species V. harveyi and V. splendidus were found as well. Staphylococci and bacilli occupied the second place in abundance after vibrios. In addition, coryneforms and enterobacteria, as well as Pseudomonas spp. and Pseudoalteromonas spp., were revealed. The composition of the water microflora was more diverse as compared with the microflora of mollusks. In the water, Bacillus spp., Vibrio spp., and Pseudomonas spp. were predominant. Brevibacterium spp. and other coryneform bacteria, as well as enterobacteria, occurred in significant amounts. In addition, Pseudoalteromonas spp., Marinococcus sp., Halobacillus sp., Shewanella sp., Sulfitobacter sp., and bacteria of the CFB cluster were noticed. The presence of pathogenic and conditionally pathogenic bacterial species in the water and mollusks is probably the reason for the high death rate of cultivated animals at the mariculture farm.     

Spermatogenesis and Testes Maturity Scale in Walleye Pollock <Emphasis Type=Italic>Theragra chalcogramma</Emphasis> (Gadidae)

In this paper walleye pollock spermatogenesis features were investigated. Male germ cells morphology and sizes were shown. The male length, gonadosomatic and hepatosomatic indexes correlated with gonadal maturity. Additional previously unidentified physiological condition TR was determined in maturity scale of walleye pollock testes.     

Genetic structure of the Iranian-speaking population of Azerbaijan from data on frequencies of immunologic and biochemical gene markers

The data on the genetic studies of Iranian-speaking populations from Azerbaijan (Talyshs and Tats) are presented. In these populations gene frequency distributions for the immunological (AB0, MN, Rhesus-D, -C, -E, P, Lewis, and Kell-Chellano) and biochemical (HP, GC, C'3, TF, 6PGD, GLO1, ESD, ACP1, and PGM1) gene markers were determined. Comparison of the genetic structure of the populations examined with the other Iranian-speaking populations (Persians and Kurds from Iran, Ossetins and Tajiks) and Azerbaijanis showed that Iranian-speaking populations from Azerbaijan were more close to Azerbaijanis, than to Iranian-speaking populations inhabiting other world regions.     

Antigenic makeup of the protective fractions of S. typhimurium]

The authors present the results of chemical and immunochemical study of 4 protective fractions (Grasset's antigens, hydroxylamine vaccines, surface antigens, and Boivin's antigens) isolated from the virulent S. typhimurium strain, and of suppressor revertant obtained from streptomycin-dependent mutant of the initial strain. Despite some differences in their chemical and antigenic composition, Grasset's antigens, surface antigens fraction, and hydroxylamine vaccine contained the main cell protective substances and could be used (after further study) as vaccines for enteral immunization. On the basis of the results of chemical analysis and the study of Boivin's antigens antigenic structure it is supposed that O-antigen was changed in the revertant strain.     

Density of distribution of nerve cells in the nuclei of the cat's trigeminal complex]

The results of calculation of nerve cells density in each of the four nuclei composing the nuclear complex of the trigeminal nerve are presented. The quantitative data were processed by non-parametrical statistical method. It was established that the whole rostral half of the trigeminal complex of the nuclei had greater density of cell distribution, than the caudal one. Especially pronounced was the difference in the density of disposition of cells between the nuclei occupying the extreme-caudal and rostral position in the general complex of nuclei. Comparison of the obtained facts with the literature data and the author's previous investigations has shown that the revealed distinctions are in agreement with pecularities of the structural functional organizations of the trigeminal complex, according to which the structure is changed from the caudal to rostral end to be more isolated from the reticular formation, and the function is changed to conduct more delicate differentiated stimuli.