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排序方式: 共有658条查询结果,搜索用时 109 毫秒
1.
眼镜蛇科蛇毒对S180,EAC腹水癌治疗作用研究 总被引:1,自引:0,他引:1
本文报道金环蛇、扁颈蛇、眼镜蛇、银环蛇蛇毒及其细胞毒对小鼠S180,EAC腹水癌的治疗作用。结果表明,蛇毒及其细胞毒在体外有明显杀灭癌细胞作用,体内有较明显的治疗作用,动物存活时间延长,接种率降低。肿瘤细胞呈现不同程度形态变化,主要为膜破裂,坏死等。治疗效应由强到弱为金环蛇毒,扁颈蛇毒,金环蛇细胞毒,眼镜蛇毒,眼镜蛇细胞毒。银环蛇毒无作用。 相似文献
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Chaomurilege Zu Yanhui Miyagi Atsuko Hashida Shin-Nosuke Ishikawa Toshiki Yamaguchi Masatoshi Kawai-Yamada Maki 《Journal of plant research》2023,136(1):97-106
Journal of Plant Research - Chloroplast-localized NAD kinase (NADK2) is responsible for the production of NADP+, which is an electron acceptor in the linear electron flow of photosynthesis. The... 相似文献
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本文对华北地区蓟属CirsiumAdans.16种植物的甲醇提取液紫外吸收光谱(UV)进行了比较研究。结果表明,蓟属植物的叶较其它部位的UV具有较好的稳定性和代表性,其光谱的特征可以作为属下分类的重要依据,其化学分类结果与形态分类基本一致。根据光谱特征,结合形态学及有关的细胞学和同工酶方面的资料,重新确立了Sect.Pseudo-Eriolepis(Nakai)Kitam.和C.segetumBge.的组级和种级地位,最后,作者进一步讨论了光谱特征在化学分类中的意义。 相似文献
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Summary A male patient with an interstitial deletion 13q14q31 is described. Our necropsy findings included a left retinoblastoma and several gross internal malformations. In this paper we reaffirm that band 13q14 is involved in cases of retinoblastoma and we propose, after studying accompanying cases of total or partial long arm trisomies 13, that the loss of specific 13q bands, from 13q14 to 13q31 is responsible for the congenital defects we are describing. 相似文献
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Characterization of interleukin 2 stimulated 65-kilodalton phosphoprotein in human T cells 总被引:3,自引:0,他引:3
We have characterized the cellular proteins which are rapidly phosphorylated by interleukin 2 (IL 2) in a human IL 2 dependent cell line. When treated with IL 2, the phosphorylation of five proteins, 65, 50, 37, 24, and 21 kDa, was found in IL 2 dependent cell lines by two-dimensional gel electrophoretic analysis. After cell conversion from an IL 2 dependent state to an IL 2 independent state, one of the five phosphoproteins, the 65-kDa protein, became constitutively phosphorylated even without addition of IL 2. Also, in other IL 2 independent cell lines, such as KUT-2 and HUT-102, constitutive phosphorylation of the 65-kDa protein occurred without IL 2-stimulation. So our researchers were focused on biochemical characterization of the 65-kDa protein. It was found that the 65-kDa protein was one of the major cellular proteins by comparing the results of two-dimensional gel electrophoretic analysis of [32P]Pi-labeled and [3H]leucine-labeled cellular proteins and peptide mapping analysis. Subcellular fractionation studies indicated that the 65-kDa protein is a cytosol protein. The 65-kDa protein was purified from cytosol of a human T cell line, and its amino acid composition and amino acid sequences of its three oligopeptides were determined. It was found that the 65-kDa protein is identical with 1-plastin. 相似文献
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Jun Mi Yang Feng Jie Wen Yiqun Su Lin Xu Tingjian Zu Chang Liu David E. Fisher Xunwei Wu 《Pigment cell & melanoma research》2020,33(1):16-29
Primary melanocytes isolated from skin and expanded in culture have been widely used for laboratory research and clinical applications. The conventional method to isolate primary melanocytes from skin usually requires about 3–4 weeks of culture for melanocytes to grow sufficiently to passage. Considering that melanocytes comprise only 3%–7% of epidermal cells in normal human skin, it would be extremely helpful to increase the isolation efficiency and shorten the initial culture time to quickly meet various application needs. Here, we report that adding Y‐27632, a Rho kinase inhibitor, into the initial culture medium for 2 days can dramatically increase the yield of melanocytes. We found that Y‐27632 can promote keratinocyte attachment and survival in the melanocyte culture system, resulting in not only better recovery, but also increased proliferation of melanocytes by a paracrine signaling pathway. More specifically, Y‐27632 significantly induced keratinocyte expression of stem cell factor, which played an important role in enhancing the growth of melanocytes. In summary, Y‐27632 could profoundly enhance the yield of primary melanocytes in the initial culture through paracrine effects on keratinocytes. 相似文献