全文获取类型
收费全文 | 93篇 |
免费 | 13篇 |
专业分类
106篇 |
出版年
2023年 | 1篇 |
2022年 | 1篇 |
2021年 | 2篇 |
2020年 | 2篇 |
2019年 | 7篇 |
2018年 | 2篇 |
2017年 | 7篇 |
2016年 | 3篇 |
2015年 | 3篇 |
2014年 | 7篇 |
2013年 | 3篇 |
2012年 | 4篇 |
2011年 | 5篇 |
2010年 | 1篇 |
2009年 | 1篇 |
2008年 | 2篇 |
2007年 | 6篇 |
2006年 | 6篇 |
2005年 | 2篇 |
2004年 | 3篇 |
2003年 | 2篇 |
2002年 | 2篇 |
2001年 | 3篇 |
2000年 | 2篇 |
1999年 | 3篇 |
1998年 | 1篇 |
1997年 | 3篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1992年 | 1篇 |
1988年 | 1篇 |
1986年 | 1篇 |
1984年 | 2篇 |
1982年 | 1篇 |
1980年 | 2篇 |
1975年 | 1篇 |
1972年 | 1篇 |
1970年 | 2篇 |
1969年 | 4篇 |
1968年 | 1篇 |
1966年 | 2篇 |
1938年 | 1篇 |
排序方式: 共有106条查询结果,搜索用时 15 毫秒
1.
2.
3.
Abstract— The activity of glutamate decarboxylase in the brain of rats during and prior to experimentally produced cerebral seizures was compared with that of control rats. An inhibition of enzyme activity during the tonic convulsions after intracisternal injection of l -glutamate or pyridoxal-5-phosphate, after audiogenic stimulation, after intraperitoneal injection of pentamethylenetetrazole and during the electroshock could be observed. During the preconvulsive stage the enzyme was strongly inhibited after an intracisternal injection of l -glutamate, l -aspartate, and after audiogenic stimulation. Only after the intracisternal injection of pyridoxal-5-phosphate the enzyme activity as compared with that of control rats was unchanged. The different effects of l -glutamate and pyridoxal-5-phosphate in vivo and in vitro on the glutamate decarboxylase are pointed out in particular. The inhibition of this enzyme in vivo is believed to be one of the possible causes of cerebral seizures. 相似文献
4.
K. Sonntag J. Schwinde A. A. de Graaf A. Marx B. J. Eikmanns W. Wiechert H. Sahm 《Applied microbiology and biotechnology》1995,44(3-4):489-495
The carbon flux distribution in the central metabolism of Corynebacterium glutamicum was studied in batch cultures using [1-13C]- and [6-13C]glucose as substrate during exponential growth as well as during overproduction of l-lysine and l-glutamate. Using the 13C NMR data in conjunction with stoichiometric metabolite balances, molar fluxes were quantified and normalised to the glucose uptake rate, which was set to 100. The normalised molar flux via the hexose monophosphate pathway was 40 during exponential growth, whereas it was only 17 during l-glutamate production. During l-lysine production, the normalised hexose monophosphate pathway flux was elevated to 47. Thus, the carbon flux via this pathway correlated with the NADPH demand for bacterial growth and l-lysine overproduction. The normalised molar flux in the tricarboxylic acid cycle at the level of 2-oxoglutarate dehydrogenase was 100 during exponential growth and 103 during l-lysine secretion. During l-glutamate formation, the normalised flux through the tricarboxylic acid cycle was reduced to 60. In contrast to earlier NMR studies with C. glutamicum, no significant activity of the glyoxylate pathway could be detected. All experiments indicated a strong in vivo flux from oxaloacetate back to phosphoenolpyruvate and/or pyruvate, which might be due to phosphoenolpyruvate carboxykinase activity in C. glutamicum. 相似文献
5.
The effects of acute stress during a parachute jump on hormonal responses were studied in 12 experienced and 11 inexperienced military parachutists. Each subject performed two jumps. Prior to and immediately after each jump blood samples were drawn and analysed for plasma levels of cortisol, prolactin, thyrotropin (TSH), somatotropin (STH), and luteinizing hormone (LH). While there was a significant increase in cortisol, prolactin and TSH levels after both jumps, no alterations could be observed in STH and LH levels. Stress-induced hormonal responses were not affected by jump experience. There was also no association between the endocrine variables and anxiety scores. 相似文献
6.
7.
Experimental design for the identification of macrokinetic models and model discrimination 总被引:2,自引:0,他引:2
An experimental design method for the identification of macrokinetic models was developed applying an extended D-optimal design criterion. The D-optimal design criterion was modified to consider variable measurement variances as well as multivariate macrokinetic models. The macrokinetics of formate dehydrogenase (FDH) production with Candida boidinii were thus identified within 10 steady state experiments in a labscale continuous stirred tank reactor (10 model parameters). Closed loop control (nutristat) was applied to set-up the operating states suggested by this experimental design method. After each set of steady state experiments the quality of macrokinetic parameters was characterized statistically. For model discrimination a parameter discrimination algorithm based on entropy formulations was adapted. Again a multivariate criterion considering variable measurement variances was developed. This discrimination algorithm was applied to discriminate the macrokinetic model of FDH production with Candida boidinii out of 10 different macrokinetic approaches. An unequivocal discrimination result could be obtained calculating model specific probabilities. These were compared with commonly used sum of squares values. (c) 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 56: 564-576, 1997. 相似文献
8.
Roman Jansen Holger Morschett Dennis Hasenklever Matthias Moch Wolfgang Wiechert Marco Oldiges 《Biotechnology progress》2021,37(4):e3144
In recent years, many fungal genomes have become publicly available. In combination with novel gene editing tools, this allows for accelerated strain construction, making filamentous fungi even more interesting for the production of valuable products. However, besides their extraordinary production and secretion capacities, fungi most often exhibit challenging morphologies, which need to be screened for the best operational window. Thereby, combining genetic diversity with various environmental parameters results in a large parameter space, creating a strong demand for time-efficient phenotyping technologies. Microbioreactor systems, which have been well established for bacterial organisms, enable an increased cultivation throughput via parallelization and miniaturization, as well as enhanced process insight via non-invasive online monitoring. Nevertheless, only few reports about microtiter plate cultivation for filamentous fungi in general and even less with online monitoring exist in literature. Moreover, screening under batch conditions in microscale, when a fed-batch process is performed in large-scale might even lead to the wrong identification of optimized parameters. Therefore, in this study a novel workflow for Aspergillus niger was developed, allowing for up to 48 parallel microbioreactor cultivations in batch as well as fed-batch mode. This workflow was validated against lab-scale bioreactor cultivations to proof scalability. With the optimized cultivation protocol, three different micro-scale fed-batch strategies were tested to identify the best protein production conditions for intracellular model product GFP. Subsequently, the best feeding strategy was again validated in a lab-scale bioreactor. 相似文献
9.
Holger Morschett Jochem Gtgens Wolfgang Wiechert Marco Oldiges 《Engineering in Life Science》2019,19(12):1006-1011
Due to their high triacylglyceride content, microalgae are intensively investigated for bio‐economy and food applications. However, lipid analysis is a laborious task incorporating extraction, transesterification and typically gas chromatographic measurement. Co‐elution induces a significant risk of fatty acid misidentification and thus, additional purification steps like thin layer chromatography are needed. Contrary to database matching approaches, solely targeted analysis is facilitated as compound identification is driven by matching retention times or indices with standard substances. In this context, a rapid workflow for the analysis of algal fatty acids is presented. In‐situ transesterification was used to simplify sample preparation and conditions were optimized towards fast processing. If results are needed at the very day of sampling, direct processing without a preceding drying step is feasible to obtain a rough estimate about the occurrence of the major compounds. Coupling gas chromatography and time‐of‐flight mass spectrometry enables untargeted analysis. Unknown compounds may be identified by structural reconstruction of their respective fragmentation patterns and by database matching to routinely avoid mismatches by co‐elution of disturbing agents. The developed workflow was successfully applied to derive the exact stereochemistry of all fatty acids from Chlorella vulgaris and a systematic shift depending on physiological state of the cells was confirmed. 相似文献
10.