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Clathrin isoform CHC22, a component of neuromuscular and myotendinous junctions, binds sorting nexin 5 and has increased expression during myogenesis and muscle regeneration
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Towler MC Gleeson PA Hoshino S Rahkila P Manalo V Ohkoshi N Ordahl C Parton RG Brodsky FM 《Molecular biology of the cell》2004,15(7):3181-3195
The muscle isoform of clathrin heavy chain, CHC22, has 85% sequence identity to the ubiquitously expressed CHC17, yet its expression pattern and function appear to be distinct from those of well-characterized clathrin-coated vesicles. In mature muscle CHC22 is preferentially concentrated at neuromuscular and myotendinous junctions, suggesting a role at sarcolemmal contacts with extracellular matrix. During myoblast differentiation, CHC22 expression is increased, initially localized with desmin and nestin and then preferentially segregated to the poles of fused myoblasts. CHC22 expression is also increased in regenerating muscle fibers with the same time course as embryonic myosin, indicating a role in muscle repair. CHC22 binds to sorting nexin 5 through a coiled-coil domain present in both partners, which is absent in CHC17 and coincides with the region on CHC17 that binds the regulatory light-chain subunit. These differential binding data suggest a mechanism for the distinct functions of CHC22 relative to CHC17 in membrane traffic during muscle development, repair, and at neuromuscular and myotendinous junctions. 相似文献
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Joumaa WH Léoty C 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》2002,172(4):329-338
The effects of 5 weeks treadmill-exercise training on isometric tension and contractile proteins were studied in intact and skinned isolated small bundles of rat skeletal soleus and extensor digitorum longus (edl) fibers. In soleus and edl muscles, 5 weeks exercise training: (i) increased twitch amplitude by 25% and 8%, respectively, without modification in the time-to-peak tension and the time constant of relaxation, (ii) increased the amplitude of K(+) contracture by 93% and 88%, respectively, and accelerated its relaxation by 17% and 43%, respectively, and (iii) increased the amplitude of caffeine contractures (soleus: 0.5 mM: 86%, 10 mM: 77%; edl: 0.5 mM: 89%, 10 mM: 87%). In conclusion, changes in contractile responses were associated with shifts in the steady state inactivation curves and in the voltage-dependent activation curve to a more negative potential, with increases in soleus and edl caffeine sensitivity, without changes in the Ca(2+) sensitivity of contractile proteins and myosin heavy chain isoforms. 相似文献
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Venus MR 《Plastic and reconstructive surgery》2007,119(1):423-4; author reply 424
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Kammoun-Krichen M Bougacha-Elleuch N Makni K Rebai M Peraldi-Roux S Rebai A Mnif M Abid M Jouida J Ayadi H 《European cytokine network》2007,18(4):196-200
Autoimmune thyroid diseases (AITDs), including Graves' disease (GD) and autoimmune hypothyroidism (AH), are inherited as complex traits. Among the genes contributing to AITDs susceptibility are genes of the IL-1 family. IL-1 regulates T and B lymphocyte maturation, including the induction of several cytokines and cytokine receptors. Therefore, disturbances of this balance may not only play a role in inflammation but also in the pathogenesis of autoimmunity. In order to investigate genetic association of IL-1 gene polymorphisms with AITDs, we performed both a familial study in a large Tunisian pedigree with high prevalence of AITDs (64 patients and 176 controls), and a case-control study (131 GD unrelated patients and 225 healthy controls). PCR and PCR-RFLP methods were used to analyse respectively a VNTR in the IL-1RN gene and three SNPs in both IL-1B genes (-511 C/T and +3954 C/T) and IL-1A (-889 C/T). The family-based association study showed an association of the IL-1B+3954 C/T polymorphism (p=0.02) and two haplotypes IL-1RN*3/C/T/T and IL-1RN*1/C/T/T (p=0.009 and p=0.047 respectively) with AITDs. The case-control study is the first study revealing a significant association of the IL-1A-889 C/T polymorphism (chi2=10.23; p=0.0014) with susceptibility to GD. Our data suggest that the IL-1 gene cluster may harbour susceptibility genes for AITDs and GD pathogenesis in the Tunisian population. 相似文献
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Sherameti I Shahollari B Venus Y Altschmied L Varma A Oelmüller R 《The Journal of biological chemistry》2005,280(28):26241-26247
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Venus J 《Biotechnology journal》2006,1(12):1428-1432
Originally, lactic acid was produced from pure substrates like glucose. Increasingly, however, agricultural feedstocks such as grains and green biomass are also being used as raw materials for the biotechnological production of lactic acid. A high-productivity lactic acid bacterium strain was selected, process parameters were optimized for the batch fermentation on a laboratory scale, and its performance at cultivation on a barley hydrolysate medium together with different supplements was examined. The present results for the cultivation of the Lactobacillus paracasei on complex nutrient broth are in the same range as those for another strain of the same species with pure glucose, de Man, Rogosa and Sharpe medium (MRS) minerals, peptone and yeast extract. Under these conditions, this strain was able to accumulate more than 100 g lactate/L in the MRS medium. Medium optimization experiments showed that the main part of the nitrogen-containing nutrients in the medium (peptone, yeast extract) can be replaced by protein extracts from green biomass (lucerne green juice). The green juice after pressing fresh biomass contains a series of nitrogen-containing compounds and inorganic salts, which are essential for cell growth. Thus, on laboratory scale, we have demonstrated that it is possible to substitute synthetic nutrients by renewable resources like cereals and green biomass without any loss of productivity. This high biomass concentration together with the number of living cells could increase the productivity to higher levels compared to the well-adapted synthetic nutrients of MRS. 相似文献
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Olteanu D Liu X Liu W Roper VC Sharma N Yoder BK Satlin LM Schwiebert EM Bevensee MO 《American journal of physiology. Cell physiology》2012,302(10):C1436-C1451
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Kevin Boldt Stela Mattiello Venus Joumaa Jeannine Turnbull Paul W.M. Fedak Walter Herzog 《Journal of Exercise Nutrition & Biochemistry》2021,25(2):8
[Purpose]The purpose of this study was to investigate the effects of a high-fat high-sucrose (HFHS) diet on previously reported adaptations of cardiac morphological and contractile properties to resistance training.[Methods]Twelve-week-old rats participated in 12-weeks of resistance exercise training and consumed an HFHS diet. Echocardiography and skinned cardiac muscle fiber bundle testing were performed to determine the structural and mechanical adaptations.[Results]Compared to chow-fed sedentary animals, both HFHS- and chow-fed resistance-trained animals had thicker left ventricular walls. Isolated trabecular fiber bundles from chow-fed resistance-trained animals had greater force output, shortening velocities, and calcium sensitivities than those of chow-fed sedentary controls. However, trabeculae from the HFHS resistance-trained animals had greater force output but no change in unloaded shortening velocity or calcium sensitivity than those of the chow-fed sedentary group animals.[Conclusion]Resistance exercise training led to positive structural and mechanical adaptations of the heart, which were partly offset by the HFHS diet. 相似文献