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In two laboratory experiments using tilapia (Oreochromis niloticus) and common carp (Cyprinus carpio), we investigated the effect of lipid content in the fish carcass on the stable carbon isotope ratio (δ13C). In both experiments, a significant negative correlation was found between lipid content in the carcass dry matter and
δ13C of total dry matter, but there was no influence on the δ13C of fat-free dry matter or lipids. As the lipid content of fish is known to vary with reproductive stage, season or nutritional
state, separate analysis of fractions of the proximate composition of dry matter will lead to more reliable results than analysing
the whole body. The differences in δ13C between diet and fish carcass (trophic shift) were different for the two species, calling for feeding trials under controlled
conditions using the species and the feeds in question before applying the stable isotope tracer technique in the analysis
of food webs.
Received: 1 December 1997 / Accepted: 1 March 1998 相似文献
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The oxygen consumption of 6 carps was measured in a flow-through respirometer when water temperature was reduced from 23 to 7°C and increased from 23 to 33°C as well as from 11 to 32°C. The metabolic reaction of Cyprinus carpio L. was assessed at 3 levels: standard, routine and spontaneous. The Standard Metabolic Rate increased and the Q10 decreased with rising temperatures. A quadratic relation was established between metabolic rates and temperature level. The enery exenditure above Standard Metabolic Rate induced by temperature changes was 6.65 ± 1.97 kJ kg-0.8 d-1 (x ± S.E., N = 6).
Costs for temperature acclimatization amounted to 29 %, 24 % and 9 % of the energy required for standard, routine and spontaneous action metabolism, respectively.
Temperaturinduzierte Stoffwechselerhöhungen bei Karpfen , Cyprinus carpio L., während Warm- und Kaltakklimatisierung
Wir haben untersucht, wie der Stoffwechsel von Cyprinus carpio im Standard-, Routine- und Spontanniveau während einer Kalt- und Warmakklimatisierung im Bereich von 7–33 °C reagiert. Dieses Verhalten wurde mit Hilfe eines Durchfluß-Respirometers ermittelt. Die "Standard Metabolic Rate" nahm zu und der Q10 verringerte sich mit steigender Temeratur.
Eine quadratische Beziehung besteht zwischen den Stoffwechselraten und dem Temperaturniveau. Die durch Temperaturänderun induzierte Stoffwechselerhöhung über die Standardrate wurde für den Meßbereich mit 6.65 ± 1.97 kJ kg-0.8 d-1 (x ± S.E., N = 6) ermittelt.
Der Anteil temperaturinduzierter Aufwendungen entsprach 29%, 24% und 9% des Standard-, Routine- und Spontanverbrauches an Energie. 相似文献
Costs for temperature acclimatization amounted to 29 %, 24 % and 9 % of the energy required for standard, routine and spontaneous action metabolism, respectively.
Zusammenfassung
Temperaturinduzierte Stoffwechselerhöhungen bei Karpfen , Cyprinus carpio L., während Warm- und Kaltakklimatisierung
Wir haben untersucht, wie der Stoffwechsel von Cyprinus carpio im Standard-, Routine- und Spontanniveau während einer Kalt- und Warmakklimatisierung im Bereich von 7–33 °C reagiert. Dieses Verhalten wurde mit Hilfe eines Durchfluß-Respirometers ermittelt. Die "Standard Metabolic Rate" nahm zu und der Q
Eine quadratische Beziehung besteht zwischen den Stoffwechselraten und dem Temperaturniveau. Die durch Temperaturänderun induzierte Stoffwechselerhöhung über die Standardrate wurde für den Meßbereich mit 6.65 ± 1.97 kJ kg
Der Anteil temperaturinduzierter Aufwendungen entsprach 29%, 24% und 9% des Standard-, Routine- und Spontanverbrauches an Energie. 相似文献
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Ulfert Rand Upneet Hillebrand Stephanie Sievers Steffi Willenberg Mario K?ster Hansj?rg Hauser Dagmar Wirth 《Nucleic acids research》2014,42(13):e109
Antiviral defence in mammals is mediated through type-I interferons (IFNs). Viruses antagonise this process through expression of IFN antagonist proteins (IAPs). Understanding and modelling of viral escape mechanisms and the dynamics of IAP action has the potential to facilitate the development of specific and safe drugs. Here, we describe the dynamics of interference by selected viral IAPs, NS1 from Influenza A virus and NS3/4A from Hepatitis C virus. We used Tet-inducible IAP gene expression to uncouple this process from virus-driven dynamics. Stochastic activation of the IFN-β gene required the use of single-cell live imaging to define the efficacy of the inhibitors during the virus-induced signalling processes. We found significant correlation between the onset of IAP expression and halted IFN-β expression in cells where IFN-β induction had already occurred. These data indicate that IAPs not only prevent antiviral signalling prior to IFN-β induction, but can also stop the antiviral response even after it has been activated. We found reduced NF-κB activation to be the underlying mechanism by which activated IFN expression can be blocked. This work demonstrates a new mechanism by which viruses can antagonise the IFN response. 相似文献
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Models for estimating food consumption in fish by analysing changes in stomach fullness over time are invariably based on a stomach evacuation rate obtained when the fish is fasting, on the assumption that this rate also applies to when the fish is feeding. However, this often is not the case in fish that feed on small particles. A new modelling approach was therefore tested, which is based not only on stomach fullness but also on gut contents. To eliminate errors arising from assimilation in the gut, titanium(IV) oxide (TiO2) was used as an indigestible marker. When applied to a dataset obtained from tilapia given several equal doses of pelleted feed over a 2.5‐h period, the new approach gave a closer true consumption estimate than a conventional model. The evacuation rate proved to be a more sensitive parameter than the ingestion rate, but the former was no longer required by the new approach for estimating ingestion, thus liberating the food consumption estimate from any errors and dependencies inherent in the evacuation rate. The new approach assumes that the digesta of previous feedings can be distinguished from those of the feeding phase being analysed and therefore needs further refinement for those cases when this does not apply. Suggestions for such refinements are also given. This new approach is expected to be equally suitable for estimating consumption in stomachless fish. 相似文献
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Gaye-Siessegger J Focken U Abel H Becker K 《Comparative biochemistry and physiology. Part A, Molecular & integrative physiology》2007,146(1):71-77
The quality of dietary protein is an important factor influencing the growth performance of fish. To evaluate the quality of protein, the variables commonly studied are the composition of the essential amino acids, the digestibility and the protein use efficiency. The goal of the present experiment was to test the effect of the dietary non-essential amino acid composition on the growth of Nile tilapia (Oreochromis niloticus). The fish were fed three purified diets differing only in their non-essential amino acid composition. The influence of the experimental diets on the growth performance, on the activity of enzymes involved in the amino acid metabolism, aspartate aminotransferase (ASAT) and alanine aminotransferase (ALAT), and on whole body delta(15)N values was investigated. Body mass, lipid, protein and energy gain differed significantly between the feeding groups. The activity of ASAT in the whole liver was significantly higher in fish with a positive protein balance compared to fish which lost protein. Whole body delta(15)N values of fish were negatively correlated with their body mass gain. Despite the poor utilisation of synthetic amino acids, the experiment indicates the importance of the dietary non-essential amino acid composition for the growth performance of fish. The study reveals the possibility to trace the utilisation of synthetic amino acids by determining the isotopic composition of dietary amino acids and tissues or whole bodies of animals. 相似文献
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Cuvin-Aralar ML Fastner J Focken U Becker K Aralar EV 《Systematic and applied microbiology》2002,25(2):179-182
Laguna de Bay, the largest freshwater lake in the Philippines, experiences periodic blooms of the cyanobacteria Microcystis aeruginosa. Blooms of these cyanobacteria in 1996, 1998 and 1999 were sampled. HPLC and MALDI-TOF mass spectrometry were used to analyze for microcystins. A total of 16 structural variants of the toxin were isolated from the samples with microcystin LR (MC-LR) as the most abundant variant in the samples from 1996 and 1999 making up 77 to 85% of the total, respectively. MC-RR was the dominant variant in the 1998 bloom making up 38%. The samples from 1996 had the highest total toxin concentration (4049 microg g(-1)) followed by those from 1998 (1577 microg g(-1)) and 1999 (649 microg g(-1)). A strain of M. aeruginosa previously isolated from the lake was also cultured in the laboratory under different nitrogen concentrations (1, 3 and 6 mg L(-1)) and elevated phosphorus concentration (0.5 mg L(-1)) to determine the influence of these factors on toxin production. A total of 9 different structural variants of microcystin were isolated from the laboratory cultures with MC-LR consisting more than 75% of the total in all treatments. No significant differences in the total toxin concentration as well as the % distribution of the different variants among treatments were observed. However, the strain of M. aeruginosa cultured in the laboratory had from 3 to 20 times higher total microcystin than those harvested from the lake. 相似文献