全文获取类型
收费全文 | 2171篇 |
免费 | 246篇 |
专业分类
2417篇 |
出版年
2023年 | 14篇 |
2022年 | 28篇 |
2021年 | 47篇 |
2020年 | 25篇 |
2019年 | 46篇 |
2018年 | 49篇 |
2017年 | 47篇 |
2016年 | 58篇 |
2015年 | 108篇 |
2014年 | 110篇 |
2013年 | 115篇 |
2012年 | 151篇 |
2011年 | 164篇 |
2010年 | 91篇 |
2009年 | 95篇 |
2008年 | 118篇 |
2007年 | 106篇 |
2006年 | 74篇 |
2005年 | 78篇 |
2004年 | 86篇 |
2003年 | 73篇 |
2002年 | 71篇 |
2001年 | 60篇 |
2000年 | 46篇 |
1999年 | 56篇 |
1998年 | 29篇 |
1997年 | 22篇 |
1996年 | 32篇 |
1995年 | 24篇 |
1994年 | 17篇 |
1993年 | 13篇 |
1992年 | 38篇 |
1991年 | 19篇 |
1990年 | 36篇 |
1989年 | 36篇 |
1988年 | 30篇 |
1987年 | 16篇 |
1986年 | 14篇 |
1985年 | 23篇 |
1984年 | 18篇 |
1983年 | 15篇 |
1981年 | 9篇 |
1980年 | 8篇 |
1979年 | 10篇 |
1978年 | 12篇 |
1977年 | 12篇 |
1976年 | 8篇 |
1974年 | 8篇 |
1973年 | 9篇 |
1968年 | 9篇 |
排序方式: 共有2417条查询结果,搜索用时 0 毫秒
1.
Cristian A. Acevedo Elizabeth Y. Sanchez Juan G. Reyes Manuel E. Young 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2010,878(3-4):449-455
It is known that skin releases volatile organic compounds to the environment, and also that its emission pattern changes with aging of the skin. It could be considered, that these compounds are intermediaries in cell metabolism, since many intermediaries of metabolic pathways have a volatile potential. In this work, a simple and non-destructive method consisting of SPME sampling and GC/MS analysis was developed to identify volatile organic emanations from cell cultures. This technique, applied to skin cells culture, indicates that the cells or cell metabolism produce several skin emissions. Chemometric analysis was performed in order to explore the relationship between a volatile profile and the senescence of cell cultures. Volatile profiles were different for cell cultures in different degrees of senescence, indicating that volatile compound patterns could be used to provide information about the age of skin cells. 相似文献
2.
Mice were immunized with dengue type 2 virus (DEN 2) under a schedule favoring the production of IgE antibody. The antibody obtained could sensitize peritoneal resident mast cells both in vitro and in vivo so that the sensitized cells were degranulated and released histamine on challenge with the DEN 2 antigen. It was also demonstrated that the antibody was cytophilic and heat-labile. The above observations suggest that the present experimental system can be used to detect anti-DEN 2 IgE antibody in mice. 相似文献
3.
Pascal Griesmar Clément Sanchez Germain Puccetti Isabelle Ledoux Joseph Zyss 《Molecular Engineering》1991,1(3):205-220
Orientation of optically nonlinear organic molecules inside sol-gel matrices upon application of an external D.C. electrical field is demonstrated for the first time. The quadratic nonlinear response of silicon oxide or transition metal oxide based gels containing organic molecules has been determined from Electric Field Induced Second Harmonic (EFISH) measurements. Large concentrations of Optically Nonlinear Organic Molecules (ONOM) have been either incorporated inside the macromolecular network or chemically bonded to the oxide backbone of the gels. These results demonstrate the feasibility of permanently poled doped sol-gel matrices. Moreover, EFISH measurements performed on organic molecules appear to be a useful tool for monitoring the changes occurring during sol-gel transformations. 相似文献
4.
The F2 progeny of a cross between a chromosome 2 multiple marker stock and an adapted cultivar of barley were analyzed for four
morphological markers and electrophoretic patterns of eight leaf isozymes. TheIdh-2 locus was linked to thePer-5 locus (27.96±5.07 cM) and to thee locus (10.26±3.13 cM). Also, thePer-5 ande loci were located on the short arm of chromosome 2. In additionIdh-2 was also located on barley chromosome 2 and was linked to thev locus (13.18±3.56 cM), which is located on the long arm of chromosome 2. Two other marker genes,li andwst,,B, were linked (26.50±5.24 cM) on chromosome 2 but segregate independently of the other loci evaluated.
This project was supported by funds from the U.S.-Spain Joint Committee for Scientific and Technological Cooperation. 相似文献
5.
In vitro neurite extension by granule neurons is dependent upon astroglial-derived fibroblast growth factor 总被引:20,自引:0,他引:20
M E Hatten M Lynch R E Rydel J Sanchez J Joseph-Silverstein D Moscatelli D B Rifkin 《Developmental biology》1988,125(2):280-289
When grown in the absence of astroglial cells, purified mouse cerebellar granule neurons survive less than 36 hr and do not extend neurites. Here we report that low concentrations of basic fibroblast growth factor (bFGF, 1-25 ng/ml) maintained the viability and promoted the differentiation of purified granule neurons. The effect of bFGF on granule cell neurite outgrowth was dose dependent. Neurite outgrowth was stimulated markedly in the presence of 1-25 ng/ml bFGF, but effects were not seen below 1 ng/ml or above 50 ng/ml. When affinity-purified antibodies against bFGF (1-5 micrograms/ml) were added either to purified granule cells or to co-cultures of neurons and astroglial cells, process extension by granule neurons was severely impaired. The inhibition of neurite outgrowth in the presence of anti-bFGF antibodies was reversed by the addition of 25 ng/ml of exogenous bFGF. In addition to neuronotrophic effects, bFGF influenced the rate of growth of the astroglial cells. This result depended on whether the astroglia were grown in isolation from neurons, where low doses of bFGF (10-25 ng) stimulated glial growth, or in coculture with neurons, where much higher doses of bFGF (100-250 ng/ml) were needed for glial mitogenesis. Immunoprecipitation of lysates from 35S-labeled cerebellar astroglial cells with anti-bFGF antibodies revealed a single band after SDS-PAGE at 18,000 Da, the molecular weight of bFGF. These results indicate that glial cells synthesize bFGF and are possibly an endogenous source of bFGF in cerebellar cultures. Thus, astroglial cells synthesize soluble factors needed for neuronal differentiation. 相似文献
6.
In order to characterize the mechanism of activation of the enzyme 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine:acetyl-CoA acetyltransferase (EC 2.3.1.67) which is the limiting step in the regulation of the synthesis of the potent inflammatory mediator 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine; homogenates from human polymorphonuclear leukocytes were incubated in the presence of the catalytic subunit of cyclic AMP-dependent protein kinase and in the presence of a partially purified phospholipid sensitive, calcium-dependent protein kinase (PrKC). The first kinase was found to enhance up to 3-fold acetyltransferase activity in a dose- and time-dependent manner. In homogenates from PMN previously stimulated with complement-coated zymosan particles, the decay of acetyltransferase activity was partially prevented by the addition of soybean trypsin inhibitor and almost completely inhibited when the homogenates were supplemented with inhibitors of alkaline phosphatase such as 50 mM KF and 100 microM paranitrophenylphosphate. Under these conditions it was possible to initiate the decay of acetyltransferase activity by adding an excess of alkaline phosphatase. Preincubation of PMN with 12-O-tetradecanoylphorbol-13-acetate previous or simultaneously to the addition of ionophore A23187 reduced the increase in acetyltransferase produced by ionophore A23187, whereas the generation of superoxide anions was enhanced. Addition of partially purified PrKC to homogenates from ionophore A23187-stimulated PMN, reduced acetyltransferase activity by 63%, whereas only a 16% inhibition was observed on homogenates from resting PMN. These data indicate the modulation of acetyltransferase activity in human polymorphonuclear leukocytes by a phosphorylation-dephosphorylation mechanism linked to cyclic AMP-dependent protein kinase. Phospholipid sensitive, calcium-dependent protein kinase seems not to be involved in the mechanism of activation, but, most probably, in the generation of negative activation signals. 相似文献
7.
A S Edelman B Xue P Sanchez G J Thorbecke 《Journal of immunology (Baltimore, Md. : 1950)》1985,135(3):2206-2212
Dimethylbenz[a]anthracene (DMBA)-induced transplantable fibrosarcomas in B2 homozygous chickens (SC line) grow progressively in normal chickens, but are rejected by chickens immunized previously with irradiated tumor cells and Corynebacterium parvum. Tumor-immune chickens resist challenge by the immunizing tumor lines as well as by some, but not all, fibrosarcoma lines. The pattern of cross-reactivity between four DMBA-induced transplantable tumor lines was examined in detail. Ability to reject a tumor challenge correlated very well (p less than 0.001) with the presence of delayed-type hypersensitivity (DTH) to that tumor. Immunization with one of two of the DMBA-induced lines tested also caused rejection of transplantable tumors developed from methylcholanthrene-induced and benzo(a)pyrene-induced primary fibrosarcomas. Although immunization with tumor caused DTH to chicken embryo fibroblasts (CEF), immunization with CEF failed to cause protective immunity or DTH to tumors. Presence of protective immunity, where tested, also correlated with the ability of spleen cells from immune donors to inhibit tumor growth in Winn tests. Humoral immunity exhibited even greater cross-reactivity than did cellular immunity. Distinct patterns of cross-reactivity were nevertheless observed with respect to the serum antibodies as detected in ELISA. Two of these patterns were also observed in several sera from primary tumor-bearing chickens, both including reactivity with CEF. Such reactivity was absent from normal chicken sera. 相似文献
8.
Management of a harem breeding colony of rhesus monkeys to reduce trauma-related morbidity and mortality 总被引:1,自引:0,他引:1
M J Kessler W T London R G Rawlins J Gonzalez H S Martinez J Sanchez 《Journal of medical primatology》1985,14(2):91-98
A management procedure was developed for a harem breeding colony of rhesus monkeys to reduce trauma-related injuries and deaths resulting from the periodic removal of pregnant monkeys for research and their subsequent return to the population. Lower morbidity and mortality rates, a reduced mean conception interval, and a higher mean conception rate occurred when monkeys were maintained in permanent harems to which returning females were reintroduced compared to new social groups formed from aggregates of unfamiliar animals. 相似文献
9.
Isolation and characterization of Pediococcus halophilus from salted anchovies (Engraulis anchoita) 总被引:3,自引:0,他引:3
M Villar A P de Ruiz Holgado J J Sanchez R E Trucco G Oliver 《Applied and environmental microbiology》1985,49(3):664-666
The presence of bacteria in salted anchovies during and at the end of the curing process was investigated. Attempts to isolate bacteria under aerobic or anaerobic conditions led to the isolation of only bacteria of the genus Pediococcus which were identified as Pediococcus halophilus. The isolates correspond to a rather heterogeneous group in which some of the members differ in some biochemical tests from the types described in the literature. 相似文献
10.