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1.
A. V. Bazhin E. N. Grigoryan N. K. Tikhomirova V. A. Poplinskaya P. P. Filippov 《Biology Bulletin》2002,29(4):348-355
The presence and localization of the calcium-binding protein recoverin, initially found in photoreceptors of the bovine eye, were immunochemically studied in retina of the new Pleurodeles waltl. Polyclonal monospecific antibodies against recoverin were raised and the methods of immunoblotting and indirect immunofluorescence were used. A protein with an apparent molecular mass of 26 kDa was found in the retina extract, which was specifically stained by the antibodies against recoverin. Localization of recoverin was studied on the retina sections: an intense reaction was found in the inner segments and a weak reaction was found in the basal part of the outer segments of photoreceptors and in Landolt's clubs of displaced bipolars. The results we obtained suggest for the first time the presence of recoverin in the retina of a representative of the Urodeles and indicate to interspecific conservativeness of this protein and differences of its localization in the retina photoreceptors in different species. The data obtained open a possibility of using recoverin as a marker protein of photoreceptors and displaced bipolars in studies of retina regeneration in newts. 相似文献
2.
O V Salamakha N P Rogatykh I V Savochkina L A Tikhomirova I L Bazarenko 《Mikrobiologiia》1989,58(5):778-784
The mycelium of Streptomyces fradiae was fractionated by differential centrifugation in a sucrose density gradient (SDG) using various samples of the inoculation material and aliquots of the cultural broth taken in the course of tylosin production. The mode of mycelium distribution in SDG made it possible to select the most active inoculation material. The mycelium was redistributed from sucrose layers with a high density to those with a lower density in the course of fermentation. The fractions differed in the antibiotic activity but none of them had an activity higher than in the control centrifuged in 30% sucrose and washed off just like the fractions. Therefore, mycelium fractionation in SDG would not elevate its antibiotic activity. The paper presents the cytological characteristics of different fractions changing in the course of fermentation. 相似文献
3.
A number of hybrid plasmids, carrying lambda genes involved in site-specific integrative recombination, have been constructed in vitro. Analysis of protein synthesis in Escherichia coli minicells has shown that Int protein is synthesized only when int gene is expressed constitutively. The plasmids RSF2124::lambda-CD, RSF2124::lambda-Cint-c57, and pInt lambda were able to integrate into the chromosome of E.coli at the attB. The integration of hybrid plasmids into the genome of bacteria has also been shown for polA1 strains restricting the autonomous replication of ColE1 type plasmids. Genetic markers of hybrid plasmids are maintained in polA1 bacteria for at least 50 generations under nonselective conditions. The Southern blotting experiments using [32P]pBR322 DNA and EcoRI fragments of E. coli polA1 chromosome carrying integrated plasmid pInt lambda demonstrated that in this strain hybrid plasmids can be observed only when integrated into the attB of the chromosome according to Campbell's model of integration. In the cells, where autonomous replication of plasmids is possible, they can be observed both in extrachromosomal and integrated states. The integration of the ColE1 replication origin into the chromosome of bacteria is not lethal for the cells. Only attP and the int gene of lambda are necessary for the integration of hybrid plasmids under conditions of effective int gene expression. If the level of Int protein synthesis is high enough, the prophage excision can be observed in the absence of Xis product. The six-fold decrease of Int protein concentration in the cell (in case of pInt lambda 2 as compared to pInt lambda 1) is critical both for integration and excision. 相似文献
4.
5.
Microrheologic properties (aggregation and deformability) were examined in young (upper fraction) and old (bottom fraction) erythrocytes separated by density gradient centrifugation at 30000 g. A significant difference in microrheologic properties of the young and old erythrocytes was revieled. Erythrocyte aggregation was studied in the plasma and serum. Aggregation of the young and old cells in plasma was higher than in serum, which points to the importance of the presence of fibrinogen in the aggregation medium. The study of aggregation kinetics showed that the absence of fibrinogen in the medium produced an insignificant effect on the rate of aggregate formation, but it dramatically affected the degree of aggregation (12 and 88% difference between aggregate number in the plasma and serum, respectively). In young erythrocytes, the difference of the degree of aggregation and kinetics of aggregate formation in the plasma and serum was not so pronounced (32 and 43%, respectively). Analysis of correlations between aggregation and deformation of the young and old erythrocytes revealed more pronounced interrelations of these parameters in the plasma compared to serum, indicating an important role of fibrinogen for the manifestation of microrheologic properties of erythrocytes. These interrelations were most pronounced during aggregate formation, which supported the bridging theory of aggregation. 相似文献
6.
Tikhomirova EI Zadnova SP Volokh OA Karpunina LV Shcherbakov AA 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》2003,(1):51-55
As the result of the chromatographic separation of Y. pestis EV membrane proteins, a protein fraction with hemagglutinating activity was obtained. The isolated preparation was glycoprotein with a molecular weight of 22 kD, contained 16% of carbohydrates and exhibited thermolabile properties. The determination of the carbohydrate specificity of this glycoprotein revealed that it belonged to the class of lectins. Changes in the content of 11 corticosteroids and the population composition of lymphocytes, as well as the detection of specific antibodies in the blood serum of guinea pigs immunized with lectin, were indicative of the fact that the preparation was sufficiently immunogenic and induced the activation of the processes of proliferation and activation of lymphocytes during immunogenesis. The lectin isolated from Y. pestis EV outer membrane may be regarded as an additional factor ensuring the contact of the pathogen with the cells of the body and as a promising component of combined plague vaccine. 相似文献
7.
Boronnikova SV Kokaeva ZG Gostimskiĭ SA Dribnokhodova OP Tikhomirova NN 《Genetika》2007,43(5):653-659
Genetic polymorphism of the Uralian relict plant species, yellow foxglove Digitalis grandiflora Mill. (family Scrophulariaceae), was examined using RAPD and ISSR techniques. A total of 149 RAPD and 74 ISSR markers were tested. The indices characterizing polymorphism and genetic diversity were calculated. The data obtained pointed to a high level of genetic variation of D. grandiflora (P95 = 65%). The cenopopulation examined was weakly differentiated with most of genetic diversity accounted by within-population differentiation. 相似文献
8.
O. S. Tikhomirova N. A. Grudinina V. I. Golubkov M. Yu. Mandelshtam V. B. Vasilyev 《Russian Journal of Genetics》2007,43(9):1053-1057
Screening of patients with familial breast cancer from St. Petersburg for BRCA1 gene mutations resulted in identification of three mutations (4146del3, 2761delA, and A622V) and two polymorphisms (P871L and S1436S). Mutations 4146del3 and 2761delA are novel, never previously described elsewhere. Deletion 2761delA produces a reading frame shift, premature protein synthesis termination and can cause predisposition for breast cancer. Deletion 4146del3 does not cause a frame shift, but can result not only in the disappearance of amino acid residue (D1343del) in the BRCA1 protein but also in alteration of folding of the protein, entailing loss of its functional activity. Two variants of nucleotide sequence observed in the number of patients were classified as DNA polymorphisms (P871L and S1436S) rather than mutations as they were not tightly associated with the increased risk of breast cancer. 相似文献
9.
Tikhomirova IA Murav'ev AV 《Rossi?skii fiziologicheski? zhurnal imeni I.M. Sechenova / Rossi?skaia akademiia nauk》2007,93(12):1382-1393
Red blood cell aggregation is a complex multiple-factor process and exerts a substantial effect on realization of basic blood function: oxygen transport. Insufficient knowledge about mechanisms of erythrocyte aggregation in normal conditions and, especially, in pathological conditions, complicates the control and correction of possible negative consequences of this process. On addition, red blood cell aggregation process is a suitable model for the elucidation of basic patterns of intercellular interactions. The paper presents contemporary data on mechanisms of erythrocyte aggregation and contribution of plasma and membrane erythrocyte properties to this process. Currently available data on intracellular signal pathways are discussed. 相似文献
10.
Thirty-four primary hybridoma clones were prepared which expressed monoclonal antibodies to the Ca2+-binding protein recoverin. Among the resulting monoclonal antibodies, two Ca2+-dependent clones (mAb3 and mAb19) recognizing recoverin were detected by solid-phase immunoenzyme assay. In the presence of Ca2+, antibodies of the mAb3 and mAb19 clones bound to recoverin several times better than in the absence of Ca2+. The mAb3 and mAb19 antibodies recognized epitopes located inside the sequences Pro61-Met91 and Pro57-Tyr64 of the recoverin molecule, respectively. The possible mechanism of the Ca2+-dependent recognition of recoverin by the prepared monoclonal antibodies is discussed.Translated from Biokhimiya, Vol. 69, No. 12, 2004, pp. 1667–1674.Original Russian Text Copyright © 2004 by Tikhomirova, Goncharskaya, Senin. 相似文献