排序方式: 共有2条查询结果,搜索用时 15 毫秒
1
1.
Aflatoxin B1 (AFB1) is a carcinogenic mycotoxin found in feeds and in airborne grain dusts. Aflatoxin B1 requires biotransformation to the AFB1-8,9 epoxide (AFBO) by a bioactivation system and subsequent covalent binding to DNA or proteins, to exert its carcinogenic potential. The lung contains cytochrome P450, prostaglandin-H-synthase, lipoxygenase, epoxide hydrolase and other bioactivation enzymes, and is thus a potential target for the effects of AFB1 via the routes of inhalation and ingestion. The A549 human epithelioid lung cell line and the methylthiazol tetrazolium (MTT) bioassay were used to investigate the cytotoxicity of AFB1 and its chemically synthesised epoxide (AFBO) in vitro. Statistical analysis of the MTT results indicated that there were overall significant differences between the control and both the AFB1-treated (p < 0.0001) and AFBO-treated cells (p = 0.00 2). However, there was no significant difference between AFB1 and AFBO-treated cells, when the entire range of concentrations were assessed against each other (p = 0.2877). Whenanalysed at each concentration, only at 0.01 mM was there a significant difference between the effects of AFB1 and AFBO (p = 0.0358). The results of this investigation show that AFB1 and AFBO are both cytotoxic in the A549 cell line.This revised version was published online in October 2005 with corrections to the Cover Date. 相似文献
2.
Thesla Palanee-Phillips Katie Schwartz Elizabeth R. Brown Vaneshree Govender Nyaradzo Mgodi Flavia Matovu Kiweewa Gonasagrie Nair Felix Mhlanga Samantha Siva Linda-Gail Bekker Nitesha Jeenarain Zakir Gaffoor Francis Martinson Bonus Makanani Sarita Naidoo Arendevi Pather Jessica Phillip Marla J. Husnik Ariane van der Straten Lydia Soto-Torres Jared Baeten 《PloS one》2015,10(6)
1