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1.
Synthesis and use of 1-(t-butyldimethylsilyloxy)benzotriazole (TBDMS-OBt) in the coupling of Fmoc-amino acid chlorides to amino free amino acid esters in homogeneous solution phase is described. The coupling required no addition of base and was fast and racemization free. Work up and isolation of products were easy. Yield, purity and 1H NMR analysis of peptides, synthesised by this method, were satisfactory. 相似文献
2.
Vommina?V.?Suresh BabuEmail author Kantharaju Subramanyam?J.?Tantry 《International journal of peptide research and therapeutics》2005,11(2):131-137
An efficient conversion of Nα-[(9–fluorenylmethyl)oxy]carbonyl (Fmoc) amino acid azides to the corresponding isocyanates using ultrasound is described. The Curtius rearrangement was accomplished using acid azides in toluene solution as well as solid powder at room temperature. All isocyanates synthesized have been obtained as crystalline solids and were characterized. Coupling of isocyanates with amino acid methyl ester hydrochloride salts in presence of N-methylmorpholine (NMM) resulted in Fmoc-protected dipeptidyl urea esters, which have been well characterized by 1H NMR, 13C NMR and mass spectrometry. 相似文献
3.
Tantry Subramanyam J. Vasanthakumar Ganga-Ramu Suresh Babu Vommina V. 《International journal of peptide research and therapeutics》2003,10(1):51-55
Summary The synthesis of peptides employing 9-fluorenylmethyl chloroformate (Fmoc-Cl) as a coupling agent has been described. The
method is simple, efficient and rapid. All the peptides have been obtained in good yield (70–95%). Furthermore, both the1H NMR and the HPLC studies on Fmoc-Phg-Phe-OMe and Fmoc-D-Phg-Phe-OMe revealed that the coupling is free from racemization. 相似文献
4.
Kadam D Bhandary S Hukkeri R Pillai V Tantry TP Kadam M 《Plastic and reconstructive surgery》2011,127(3):1394-5; author reply 1395-6
5.
Mudasir A. Tantry Javid A. Dar Mohammad A. Khuroo Abdul S. Shawl 《Phytochemistry letters》2012,5(2):253-257
Chemical investigation of chloroform–ethyl acetate extract from the roots of Paeonia emodi yielded four hitherto unknown noroleanane triterpenoids (1–4) together with four known compounds (5–8). Their structures were established by analysis of spectroscopic data. Compounds 1–8 were evaluated for cytotoxic activities against human cancer cell lines A549, HL-60, HCT116 and ZR-75-30. Compounds 1, 2, 3, 6 and 7 showed modest cytotoxicity against HL-60, HCT116 and ZR-75-30. 相似文献
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7.
Differential cytokine expression in skin graft healing in inducible nitric oxide synthase knockout mice 总被引:1,自引:0,他引:1
Inducible nitric oxide synthase (iNOS) and its product, nitric oxide, have been shown to play important roles in wound biology. The present study was performed to investigate the role of iNOS in modulating the cytokine cascade during the complex process of skin graft wound healing.Fifteen iNOS-knockout mice and 15 wild-type C57BL/6J mice were subjected to autogenous 1-cm2 intrascapular full-thickness skin grafts. Three animals in each group were killed on postoperative days 3, 5, 7, 10, and 14. Specimens were then analyzed using nonisotopic in situ hybridization versus mRNA of tumor growth factor-beta1, vascular endothelial growth factor, iNOS, endothelial nitric oxide synthase (eNOS), tumor necrosis factor-alpha, and basic fibroblast growth factor, as well as positive and negative control probes. Positive cells in both grafts and wound beds were counted using a Leica microgrid. Scar thickness was measured with a Leica micrometer. Data were analyzed using the unpaired Student's t test.Expression of iNOS was 2- to 4-fold higher in knockout mice than in wild-type mice on postoperative days 5, 7, and 14. Expression of eNOS was 2- to 2.5-fold higher in knockout mice than in wild-type mice on postoperative days 5 and 7. Tumor necrosis factor-alpha expression was 2- to 7-fold higher in knockout mice than in wild-type mice on all postoperative days. In contrast, expression levels of angiogenic/fibrogenic cytokines (vascular endothelial growth factor, basis fibroblast growth factor, and tumor growth factor-beta1) were 2.5- to 4-fold higher in wild-type mice than in knockout mice. Scars were 1.5- to 2.5-fold thicker in knockout mice than in wild-type mice at all time points. All of the above results represent statistically significant differences (p < 0.05).Significantly different patterns of cytokine expression were seen in knockout and wild-type mice. Although the scar layer was thicker in knockout mice, it showed much greater infiltration with inflammatory cells. These data further delineate the modulatory effect of iNOS and nitric oxide in healing skin grafts. 相似文献
8.
Tantry Subramanyam J. Babu Vommina V. Suresh 《International journal of peptide research and therapeutics》2002,9(1):35-41
Summary Synthesis and use of 1-(t-butyldimethylsilyloxy)benzotriazole (TBDMS-OBt) in the coupling of Fmoc-amino acid chlorides to
amino free amino acid esters in homogeneous solution phase is described. The coupling required no addition of base and was
fast and racemization free. Work up and isolation of products were easy. Yield, purity and1H NMR analysis of peptides, synthesised by this method, were satisfactory. 相似文献
9.
Subramanyam J. Tantry Ganga-Ramu Vasanthakumar Vommina V. Suresh Babu 《Letters in Peptide Science》2003,10(1):51-55
The synthesis of peptides employing 9-fluorenylmethyl chloroformate(Fmoc-Cl) as a coupling agent has been described. The method is simple, efficient and rapid. All the peptides have been obtainedin good yield (70–95%). Furthermore, both the 1H NMR and the HPLC studies on Fmoc-Phg-Phe-OMe and Fmoc-D-Phg-Phe-OMe revealed that the coupling is free from racemization. 相似文献
10.
Sreekanth Ramachandran Manoranjan Panda Kakoli Mukherjee Nilanjana Roy Choudhury Subramanyam J. Tantry Chaitanya Kumar Kedari Vasanthi Ramachandran Sreevalli Sharma V.K. Ramya Supreeth Guptha Vasan K. Sambandamurthy 《Bioorganic & medicinal chemistry letters》2013,23(17):4996-5001
Imidazo[1,2-a]pyridine-8-carboxamides as a novel antimycobacterial lead were generated by whole cell screening of a focused library against Mycobacterium tuberculosis. Herein, we describe the synthesis and structure activity relationship evaluation of this class of inhibitors and the optimization of physicochemical properties. These are selective inhibitors of Mycobacterium tuberculosis with no activity on either gram positive or gram negative pathogens. 相似文献