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Akira Murata Mamoru Odaka Syoichi Mukuno 《Bioscience, biotechnology, and biochemistry》2013,77(2):477-478
Some physicochemical properties and substrate specificity of acid protease B isolated from Scytalidium lignicolum were investigated.The molecular weight determined by the sedimentation equilibrium and sedimentation velocity method was 21,000 and 19,000~20,000, respectively. The isoelectric point was determined as 3.0 using the Tiselius electrophoresis apparatus, 3.2 by isoelectric focusing, respectively.The enzyme did not contain histidine and was composed of 188 amino acid residues. Substrate specificity against various synthetic peptides was different from those of the acid proteases which were inactivated by S–PI and DAN. 相似文献
2.
Syoichi Yamashita Katsushi Furuno Hiromu Kawasaki Yutaka Gomita Harumi Yoshinaga Yasuko Yamatogi Shunsuke Ohtahara 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》1995,670(2):354-357
A simple and rapid method for the quantitation of concentrations of lamotrigine, a novel antiepileptic, in human serum was developed with high-performance liquid chromatography, using a solid-phase extraction technique. The mobile phase was composed of acetonitrile-10 mM phosphate buffer (pH 3.5) containing 5 mM sodium octanesulphonate (27:73, v/v), and components were detected at 265 nm. Retention times of acetanilide as an internal standard and lamotrigine were 3.4 and 10.3 min, respectively. The coefficients of variation were 3.1–4.5% and 4.4–9.8% for the within-day and between-day precision estimates, respectively. The extraction recovery of lamotrigine added to blank serum was 86–107%. The quantitation limit of lamotrigine was ca.0.2 μg/ml in 100 μl of serum. These results suggest that the method employed in this study is useful for the routine monitoring of sereum concentrations of lamotrigine in epileptic patients. 相似文献
3.
Takashi Okabe Megumi Kumagai Yoshihiro Nakajima Suguru Shirotake Kiichiro Kodaira Masafumi Oyama Munehisa Ueno Masaaki Ikeda 《PloS one》2014,9(10)
In mammals, the circadian rhythm central generator consists of interactions among clock genes, including Per1/2/3, Cry1/2, Bmal1, and Clock. Circadian rhythm disruption may lead to increased risk of cancer in humans, and deregulation of clock genes has been implicated in many types of cancers. Among these genes, Per2 is reported to have tumor suppressor properties, but little is known about the correlation between Per2 and HIF, which is the main target of renal cell carcinoma (RCC) therapy. In this study, the rhythmic expression of the Per2 gene was not detectable in renal cancer cell lines, with the exception of Caki-2 cells. In Caki-2 cells, HIF1α increased the amplitude of Per2 oscillation by directly binding to the HIF-binding site located on the Per2 promoter. These results indicate that HIF1α may enhance the amplitude of the Per2 circadian rhythm. 相似文献
4.
Morisaki T Matsumoto K Onishi H Kuroki H Baba E Tasaki A Kubo M Nakamura M Inaba S Yamaguchi K Tanaka M Katano M 《Human cell》2003,16(4):175-182
Effective adoptive cancer immunotherapy depends on an ability to generate tumor-antigen-presenting cells and tumor-reactive effector lymphocytes and to deliver these effector cells to the tumor. Dendritic cells (DCs) are the most potent antigen-presenting cells, capable of sensitizing T cells to new and recall antigens. Many studies have shown that tumors express unique proteins that can be loaded on DCs to trigger an immune response. The current experimental and clinical statuses of adoptive transfer of tumor antigen-pulsed DCs and vaccine-primed activated T cells are summarized herein. Clinical trials of antigen-pulsed DCs have been conducted in patients with various types of cancer, including non-Hodgkin lymphoma, multiple myeloma, prostate cancer, renal cell carcinoma, malignant melanoma, colorectal cancer, and non-small cell lung cancer. These studies have shown that antigen-loaded DC vaccination is safe and promising for the treatment of cancer. In addition, tumor vaccine-primed T cells have been shown to induce antitumor activity in vivo. Several clinical studies are being conducted on the use of vaccine-primed T cells such as tumor-drainage lymph node. It is reasonable to consider using both tumor antigen-pulsed DCs and vaccine-primed lymphocytes as adjuvants. We are now investigating the use of autologous whole tumor antigen-pulsed DCs and the DC vaccine-primed activated lymphocytes in patients with multiple metastasis of solid tumors. 相似文献
5.
Souei Sekiya Teruyo Kaiho Syoichi Shirotake Hiroshi Iwasawa Noriyuki Inaba Makoto Kawata Koji Higaki Hideo Ishige Hiroyoshi Takamizawa Masako Minamihisamatsu Tsuguo Kuwata 《In vitro cellular & developmental biology. Plant》1983,19(6):489-494
Summary A human nongestational choriocarcinoma cell line of ovarian origin (IMa) was established in vitro. This cell line had been
subcultured serially more than 22 times over 18 months. Small polygonal cells with a prominent nucleus were dominant and a
sparsity of cytoplasmic organelles was an ultrastructural characteristic of the IMa cells. The production and secretion of
human chorionic gonadotropin and its subunits were identified by radioimmunoassay. The IMa cells were transplantable in the
hamster cheek pouch and the histological diagnosis was choriocarcinoma. A newly established ovarian choriocarcinoma cell line
can be considered useful for clarifying the biological differences between nongestational and gestational choriocarcinoma
cells.
This research was supported in part by a Grant-in-Aid for Cancer Research from both the Ministry of Health and Welfare and
the Ministry of Education, Science, and Culture of Japan. 相似文献
6.
Wu Hao Syoichi Tashiro Tomoka Hasegawa Yuiko Sato Tami Kobayashi Toshimi Tando Eri Katsuyama Atsuhiro Fujie Ryuichi Watanabe Mayu Morita Kana Miyamoto Hideo Morioka Masaya Nakamura Morio Matsumoto Norio Amizuka Yoshiaki Toyama Takeshi Miyamoto 《The Journal of biological chemistry》2015,290(28):17106-17115
Diabetes mellitus (DM) is frequently accompanied by complications, such as peripheral nerve neuropathy. Schwann cells play a pivotal role in regulating peripheral nerve function and conduction velocity; however, changes in Schwann cell differentiation status in DM are not fully understood. Here, we report that Schwann cells de-differentiate into immature cells under hyperglycemic conditions as a result of sorbitol accumulation and decreased Igf1 expression in those cells. We found that de-differentiated Schwann cells could be re-differentiated in vitro into mature cells by treatment with an aldose reductase inhibitor, to reduce sorbitol levels, or with vitamin D3, to elevate Igf1 expression. In vivo DM models exhibited significantly reduced nerve function and conduction, Schwann cell de-differentiation, peripheral nerve de-myelination, and all conditions were significantly rescued by aldose reductase inhibitor or vitamin D3 administration. These findings reveal mechanisms underlying pathological changes in Schwann cells seen in DM and suggest ways to treat neurological conditions associated with this condition. 相似文献
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Masahiro Moriyama Syoichi Yamashita Haruyo Domoto Katsushi Furuno Hiroaki Araki Yutaka Gomita 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》1999,723(1-2)
Plasma phenobarbital (PB) concentrations in rat offspring were determined using a 9 μl capillary by high-performance liquid chromatography (HPLC). Capillary plasma which was put into a Bond Elut® cartridge column by using 1 ml of 0.01 M KH2PO4 was applied to the column with 50 μl of 2 μg/ml of acetanilide (internal standard, I.S.). After washing the column, PB and I.S. were eluted with methanol and injected into the HPLC system. There were excellent linear correlation between the amount of PB and length of the capillary at three different concentrations. Calibration for PB was linear in the range of 0–50 μg/ml. The coefficients of variation were 3.4–5.0% and 5.9–7.5% in the within-day and between-day assays, respectively. The extraction recovery rates were 87.5–105.4%. By this method, it was possible to measure plasma PB concentrations in rat offspring without killing. These results suggested that this method is very useful to determine the plasma PB concentration derived from mother’s milk in newborn rats. 相似文献
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