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ROS signaling: the new wave? 总被引:8,自引:0,他引:8
Mittler R Vanderauwera S Suzuki N Miller G Tognetti VB Vandepoele K Gollery M Shulaev V Van Breusegem F 《Trends in plant science》2011,16(6):300-309
Reactive oxygen species (ROS) play a multitude of signaling roles in different organisms from bacteria to mammalian cells. They were initially thought to be toxic byproducts of aerobic metabolism, but have now been acknowledged as central players in the complex signaling network of cells. In this review, we will attempt to address several key questions related to the use of ROS as signaling molecules in cells, including the dynamics and specificity of ROS signaling, networking of ROS with other signaling pathways, ROS signaling within and across different cells, ROS waves and the evolution of the ROS gene network. 相似文献
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Multiple models for Rosaceae genomics 总被引:5,自引:0,他引:5
Shulaev V Korban SS Sosinski B Abbott AG Aldwinckle HS Folta KM Iezzoni A Main D Arús P Dandekar AM Lewers K Brown SK Davis TM Gardiner SE Potter D Veilleux RE 《Plant physiology》2008,147(3):985-1003
The plant family Rosaceae consists of over 100 genera and 3,000 species that include many important fruit, nut, ornamental, and wood crops. Members of this family provide high-value nutritional foods and contribute desirable aesthetic and industrial products. Most rosaceous crops have been enhanced by human intervention through sexual hybridization, asexual propagation, and genetic improvement since ancient times, 4,000 to 5,000 B.C. Modern breeding programs have contributed to the selection and release of numerous cultivars having significant economic impact on the U.S. and world markets. In recent years, the Rosaceae community, both in the United States and internationally, has benefited from newfound organization and collaboration that have hastened progress in developing genetic and genomic resources for representative crops such as apple (Malus spp.), peach (Prunus spp.), and strawberry (Fragaria spp.). These resources, including expressed sequence tags, bacterial artificial chromosome libraries, physical and genetic maps, and molecular markers, combined with genetic transformation protocols and bioinformatics tools, have rendered various rosaceous crops highly amenable to comparative and functional genomics studies. This report serves as a synopsis of the resources and initiatives of the Rosaceae community, recent developments in Rosaceae genomics, and plans to apply newly accumulated knowledge and resources toward breeding and crop improvement. 相似文献
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Coordinated Activation of Programmed Cell Death and Defense Mechanisms in Transgenic Tobacco Plants Expressing a Bacterial Proton Pump 总被引:17,自引:3,他引:17 下载免费PDF全文
In plants, programmed cell death is thought to be activated during the hypersensitive response to certain avirulent pathogens and in the course of several differentiation processes. We describe a transgenic model system that mimics the activation of programmed cell death in higher plants. In this system, expression of a bacterial proton pump in transgenic tobacco plants activates a cell death pathway that may be similar to that triggered by recognition of an incompatible pathogen. Thus, spontaneous lesions that resemble hypersensitive response lesions are formed, multiple defense mechanisms are apparently activated, and systemic resistance is induced in the absence of a pathogen. Interestingly, mutation of a single amino acid in the putative channel of this proton pump renders it inactive with respect to lesion formation and induction of resistance to pathogen challenge. This transgenic model system may provide insights into the mechanisms involved in mediating cell death in higher plants. In addition, it may also be used as a general agronomic tool to enhance disease protection. 相似文献
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Jerzy?NowakEmail author Vladimir?Shulaev 《In vitro cellular & developmental biology. Plant》2003,39(2):107-124
Summary Production of high-quality, vigorous tissue-culture-derived propagules requiles efficient ways for the enhancement of their
post-transplanting ability for water management, photosynthesis, and resistance to diseases. Certain molecules, environmental
factors, microorganisms, or their parts, can pre-sensitize cellular metabolism of plants, so upon exposure to stress these
pre-sensitized, or ‘primed’, plants are able to respond quicker, and to a higher degree than nonprimed, and thus cope better
with the challenge. In this review we propose the adoption of the term ‘priming’ for tissue culture propagation and outline
the approaches to in vitro propagule priming, based on the changes to the growth enviroment (chemical, physical, and biological) prior to and/or upon
transplanting. Major emphasis has been placed on in vitro and ex vitro biopriming (priming with beneficial microorganisms). 相似文献
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J. J. Ruiz-Rojas D. J. Sargent V. Shulaev A. W. Dickerman J. Pattison S. H. Holt A. Ciordia Richard E. Veilleux 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,121(3):449-463
As part of a program to develop forward and reverse genetics platforms in the diploid strawberry [Fragaria vesca L.; (2n = 2x = 14)] we have generated insertional mutant lines by T-DNA mutagenesis using pCAMBIA vectors. To characterize the T-DNA insertion
sites of a population of 108 unique single copy mutants, we utilized thermal asymmetric interlaced PCR (hiTAIL-PCR) to amplify
the flanking region surrounding either the left or right border of the T-DNA. Bioinformatics analysis of flanking sequences
revealed little preference for insertion site with regard to G/C content; left borders tended to retain more of the plasmid
backbone than right borders. Primers were developed from F. vesca flanking sequences to attempt to amplify products from both parents of the reference F. vesca 815 × F. bucharica 601 mapping population. Polymorphism occurred as: presence/absence of an amplification product for 16 primer pairs and different
size products for 12 primer pairs, For 46 mutants, where polymorphism was not found by PCR, the amplification products were
sequenced to reveal SNP polymorphism. A cleaved amplified polymorphic sequence/derived cleaved amplified polymorphism sequence
(CAPS/dCAPS) strategy was then applied to find restriction endonuclease recognition sites in one of the parental lines to
map the SNP position of 74 of the T-DNA insertion lines. BLAST search of flanking regions against GenBank revealed that 46
of 108 flanking sequences were close to presumed strawberry genes related to annotated genes from other plants. 相似文献
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Pisciotta JM Coppens I Tripathi AK Scholl PF Shuman J Bajad S Shulaev V Sullivan DJ 《The Biochemical journal》2007,402(1):197-204
The intraerythrocytic malaria parasite constructs an intracellular haem crystal, called haemozoin, within an acidic digestive vacuole where haemoglobin is degraded. Haem crystallization is the target of the widely used antimalarial quinoline drugs. The intracellular mechanism of molecular initiation of haem crystallization, whether by proteins, polar membrane lipids or by neutral lipids, has not been fully substantiated. In the present study, we show neutral lipid predominant nanospheres, which envelop haemozoin inside Plasmodium falciparum digestive vacuoles. Subcellular fractionation of parasite-derived haemozoin through a dense 1.7 M sucrose cushion identifies monoacylglycerol and diacylglycerol neutral lipids as well as some polar lipids in close association with the purified haemozoin. Global MS lipidomics detects monopalmitic glycerol and monostearic glycerol, but not mono-oleic glycerol, closely associated with haemozoin. The complex neutral lipid mixture rapidly initiates haem crystallization, with reversible pH-dependent quinoline inhibition associated with quinoline entry into the neutral lipid microenvironment. Neutral lipid nanospheres both enable haem crystallization in the presence of high globin concentrations and protect haem from H2O2 degradation. Conceptually, the present study shifts the intracellular microenvironment of haem crystallization and quinoline inhibition from a polar aqueous location to a non-polar neutral lipid nanosphere able to exclude water for efficient haem crystallization. 相似文献