GINS2 was regulated by lncRNA XIST/miR-23a-3p to mediate proliferation and apoptosis in A375 cells

Molecular and Cellular Biochemistry - Melanoma ranks second in aggressive tumors, and the occurrence of metastasis in melanoma results in a persistent drop in the survival rate of patients....     

地黄育种研究进展

地黄是一种玄参科药用植物,是我国著名的"四大怀药"之一,具有重要的药用价值和经济价值。该文综述了地黄的引种、选择育种、杂交育种、组织培养辅助育种、转基因育种和DNA分子标记辅助育种等育种研究的成果,以期为地黄育种工作者提供参考。     

Rapid cold-hardening protects <Emphasis Type="Italic">Drosophila melanogaster</Emphasis> from cold-induced apoptosis

The rapid cold-hardening (RCH) response increases the cold tolerance of insects by protecting against non-freezing, cold-shock injury. Apoptosis, or programmed cell death, plays important roles in development and the elimination of sub-lethally damaged cells. Our objectives were to determine whether apoptosis plays a role in cold-shock injury and, if so, whether the RCH response protects against cold-induced apoptosis in Drosophila melanogaster. The present study confirmed that RCH increased the cold tolerance of the adults at the organismal level. No flies in the cold-shocked group survived direct exposure to ‒7°C for 2 h, whereas significantly more flies in the RCH group survived exposure to ‒7°C for 2 h after a 2-h exposure to 5°C. We used a TUNEL assay to detect and quantify apoptotic cell death in five groups of flies including control, cold-shocked, RCH, heat-shocked (37.5°C, 30 min), and frozen (‒20°C, 24 h) and found that apoptosis was induced by cold shock, heat shock, and freezing. The RCH treatment significantly improved cell viability by 38% compared to the cold-shocked group. Cold shock-induced DNA fragmentation shown by electrophoresis provided further evidence for apoptosis. SDS-PAGE analysis revealed an RCH-specific protein band with molecular mass of ∼150 kDa. Western-blotting revealed three proteins that play key roles in the apoptotic pathway: caspase-9-like (apoptotic initiator), caspase-3-like (apoptotic executioner) and Bcl-2 (anti-apoptotic protein). Consequently, the results of this study support the hypothesis that the RCH response protects against cold-shock-induced apoptosis.     

常巢蛾属一新种记述(鳞翅目,巢蛾科)(英文)

记述了中国常巢蛾属Euhyponomeuta Toll,19411新种,即郑氏常巢蛾Euhyponomeuta zhengi sp.nov.,提供了新种的成虫及雄性外生殖器特征图。新种雄性外生殖器与圆腹常巢蛾E.rotunda Jin et Wang,2009相似,可通过尾突较宽且两侧平行、颚形突腹板宽舌状与其区分,后者尾突基部宽,端部渐窄,颚形突腹板三角形或半圆形。模式标本保存在南开大学生命科学学院昆虫标本室。     

锦织蛾属二新种和中国二新纪录种(鳞翅目,织蛾科)(英文)

记述锦织蛾属PromalactisMeyrick4个种,包括新种掌锦织蛾P.palmata sp.nov.和仿丽头锦织蛾P.subcolacephala sp.nov.,中国新纪录种西锦织蛾P.sineviLvovsky,1986和斯锦织蛾P.svetlanaeLvovsky,1985。文中提供了每个种的成虫和外生殖器特征图。掌锦织蛾,新种Promalactis palmata sp.nov.(图1,5,9)本种外形和外生殖器特征与显锦织蛾P.epistactaMeyrick,1908近似,但本种雄性外生殖器抱器背基部突起呈叶状,抱器瓣端部1/3近似掌状,阳茎基环侧叶末端达背兜2/3处,阳茎具2枚角状器。显锦织蛾P.epistacta雄性外生殖器抱器背基部突起柳条状,抱器瓣端部钩状,阳茎基环侧叶不明显,阳茎具1枚角状器。正模♂,贵州省绥阳县宽阔水保护区,海拔1500m,2010-08-12,杨琳琳采,外生殖器玻片号DZH10243。副模:2♂♂,3♀♀,2010-08-10~13,其它资料同正模,1♂,1♀,2010-08-16,杜喜翠采,采集地点同正模(外生殖器玻片号DZH10244♀,DZH10245♀,DZH10247♂,DZH10249♀,DZH10250♂)。分布:中国(贵州)。仿丽头锦织蛾,新种Promalactis subcolacephala sp.nov.(图2,6)本种与丽头锦织蛾P.colacephalaWang,LietZheng,2000非常近似,但雄性外生殖器抱器瓣末端背面有1钩状突起,阳茎末端无刺状突起。丽头锦织蛾P.colacephala雄性外生殖器抱器瓣末端背面有1直的突起,阳茎末端有1弯曲的刺状突起。正模♂,贵州省绥阳县宽阔水保护区,海拔840m,2010-06-09,杨琳琳采,外生殖器玻片号DZH10335。副模1♂,2010-06-08,其它资料同正模(外生殖器玻片号DZH10253)。分布:中国(贵州)。西锦织蛾Promalactis sineviLvovsky,1986中国新纪录(图3,7,10)Promalactis sineviLvovsky,1986:39.分布:中国(北京、河北、山西、辽宁);俄罗斯(远东)。斯锦织蛾Promalactis svetlanaeLvovsky,1985中国新纪录(图4,8,11)Promalactis svetlanae Lvovsky,1985:101;ParketPark,1998:57.分布:中国(黑龙江);俄罗斯(远东)。     

High temperature pulses decrease indirect chilling injury and elevate ATP levels in the flesh fly, Sarcophaga crassipalpis

Indirect chilling injury commonly occurs during long-term exposure to low temperature in many organisms including insects. A previous study revealed increased rates of survival and reduced cold injury in flesh flies, Sarcophaga crassipalpis, that experienced an intermittent pulse of high temperature during a low-temperature regiment. We extended these studies by determining survival rates and ATP levels for flies that had undergone continuous long-term exposure at 0 °C versus those experiencing a 24-h warming pulse of either 15 or 20 °C. Survival among flies that had undergone a warming pulse was significantly greater than for flies that were maintained continuously at 0 °C. Furthermore, ATP levels of flies that had experienced a warming pulse were significantly higher than those of flies maintained at 0 °C. These data suggest that brief warming pulses during long-term cold storage allow regeneration of energy reserves that promote survival and reduce indirect chilling injury.     

PCR鉴定时的微量DNA快速制备

用基因组微量DNA快速提取方法,从胡萝卜转化再生株样品中快速提取了基因组DNA,并以此为模板进行胡萝卜转化再生株的PCR快速检测的结果表明,这是转基因时PCR检测的一种快速、简便、有效方法。     

Rapid cold-hardening increases membrane fluidity and cold tolerance of insect cells

The rapid cold-hardening (RCH) response not only confers dramatic protection against cold-shock (non-freezing) injury, but also "instantaneously" enhances organismal performance. Since cold-shock injury is associated with damage to the cell membrane, we investigated the relationship between RCH and changes in cold tolerance and membrane fluidity at the cellular level. None of the adult flies (Sarcophaga bullata) in the cold-shocked treatment group survived direct transfer to -8 degrees C for 2 h; in contrast, 64.5% of flies in the RCH group survived exposure to -8 degrees C. Differences between the treatment groups also were reflected at the cellular level; only 21.3% of fat body cells in the cold-shocked group survived compared to 68.5% in the RCH group. Using 31P solid-state NMR spectroscopy, we determined that membrane fluidity increased concurrently with rapid cold-hardening of fat body cells. This result suggests that membrane characteristics may be modified very rapidly to protect cells against cold-shock injury.     

遮荫对水曲柳幼苗细根衰老的影响

细根周转对森林生态系统碳地下分配和养分循环具有重要影响,而衰老是细根周转过程中最重要的阶段。根据“源-汇”理论,细根衰老受碳向细根分配的影响。为此,该研究通过控制水曲柳(Fraxinus mandshurica)苗木向根系的碳分配的遮荫处理试验,采用树木生理分析技术,重点研究了在光合产物供应停止情况下水曲柳幼苗根系的生理变化(即根尖、1级根到3级根的细胞活力、可溶性糖含量、可溶性蛋白含量和膜透性的变化)。目的是从生理水平上证实:1)碳分配对细根衰老产生怎样的影响;2)细根衰老的顺序是否与分支顺序相反。实验结果表明,遮荫处理使细根活力、可溶性糖含量、可溶性蛋白含量显著降低和膜透性增大,导致细根出现明显衰老。从根系顶端向基部随着根序增加,细胞活力、可溶性糖含量和可溶性蛋白含量提高,膜透性降低,从生理水平上表明细根衰老具有逐渐变化的顺序性,并且这种顺序性与根发育的顺序性相反。