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N A Rodionova G V Semisotnov V P Kutyshenko V N Uverski? I A Bolotina 《Molekuliarnaia biologiia》1989,23(3):683-692
It has been shown by 1H-NMR, circular dichroism, fluorescence and viscometry techniques that equilibrium unfolding of carbonic anhydrase B (a one-domain globular protein) in urea guanidine hydrochloride consists of two sequential stages. The first stage is connected with a decrease of intramolecular interactions, stabilizing the rigid tertiary structure and with the increase of mobility of aliphatic side chain groups. At the second stage the decrease of protein secondary structure and hydrophobic interactions take place as well as the increase of mobility of massive aromatic side chain groups. 相似文献
3.
L B Pinchuk Ia I Serkiz N K Rodionova G G Pukhova N A Druzhina G N Koval' A I Lipskaia V N Maslenny? S T Malinari 《Radiobiologiia》1991,31(5):635-641
It has been shown that rats born during the first months after the Chernobyl A.P.S. disaster exhibit essential changes in the peripheral blood and bone marrow haemopoiesis throughout the entire lifetime. Rats brought up in Chernobyl from the age of three months on display even more pronounced changes. It is assumed that the changes in the haemopoiesis develop due to the continuous influence of low-level radiation of different quality and are attributed to the effect of the incorporated radionuclides. 相似文献
4.
N V Rodionova 《Tsitologiia》1985,27(9):995-1000
Using electron microscopy and 3H-thymidine autoradiography it has been shown that osteoclasts of rats and rabbits are formed by fusion of cell precursors which become members of the osteoclasts 14 hours following S-phase. Monoblasts and promonocytes are considered as DNA-synthesizing forms. A single injection of parathormone in a dose of 10 units/100 g of body weight leads to an increase in the fractions of DNA-synthesizing cells. Monocytes and macrophages appear to be direct osteoclast precursors in resorption zones. As a result of their fusion there appear "unfixed" polynuclear macrophages (young osteoclasts). The number of osteoclast nuclei increases also at the expense of little-differentiated phagocytes, the inclusion of the latter takes place at different stages of the life span of osteoclasts depending on the intensity of bone resorption processes. The structural characteristics of fusion of cell precursors are considered. 相似文献
5.
Ultrastructurometry has revealed that daily entry of lead acetate in a dose of 50-100 mg/kg per body weight for 1-2.5 months increases chromatin condensation in nuclei, promotes swelling and myelin-like degeneration of the mitochondria, hyperplasia of the endoplasmic net, as well as appearance of the "enlightment" zones in the cytoplasm of endotheliocytes and perivascular cells of the capillaries from a metaphyseal zone of the rat femur. These changes are a consequent of the edema of cells and of the decrease in their biosynthetic activity. 相似文献
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K J Atabekov L G Tyulkina O V Karpova V G Metelev N P Rodionova Z A Shabarova J G Atabekov 《FEBS letters》1988,232(1):96-98
The TMV RNA molecule can be cleaved at a single site by RNase H directed by chimeric oligo(deoxyribo-ribo)nucleotide with an internucleotide pyrophosphate bond. 相似文献
8.
Detection of Treponema pallidum DNA and RNA in clinical material from patients with syphilis at different stages 总被引:2,自引:0,他引:2
9.
J Daugherty TM Evans T Skillom LE Watson NP Money 《Fungal genetics and biology : FG & B》1998,24(3):354-363
Classical studies on spore release within the Saprolegniaceae (Oomycetes) led to the proposition that different mechanisms of sporangial emptying represent steps in an evolutionary transition series. We have reevaluated this idea in a phylogenetic framework using internal transcribed spacer sequences of four genera. These data were compared with the response to osmotic stress exhibited by each taxon. Saprolegnia emerges as the most basal genus, sister to Achlya, Thraustotheca, and Dictyuchus. Achlya and Thraustotheca are most closely related, while Dictyuchus appears to have evolved along a separate evolutionary lineage. The resulting phylogenetic framework is consistent with the idea that the mechanism of sporangial emptying exhibited by Saprolegnia represents the plesiomorphic condition from which the other mechanisms were derived independently. These alternative mechanisms of spore release may have resulted from a small number of mutations that inhibited axonemal development and altered the temporal and spatial expression of lytic enzymes that degrade the sporangial wall. Copyright 1998 Academic Press. 相似文献
10.
V. V. Voznessenskaya M. A. Klyuchnikova E. I. Rodionova T. K. Laktionova I. G. Kvasha A. B. Klinov A. E. Voznesenskaya 《Biology Bulletin》2018,45(5):485-489
We have tested the original version of the University of Pennsylvania Smell Identification Test (UPSIT) on a population of Central Russia including groups of different ages residing in the city area and in the rural part of the country. Our results demonstrate that the UPSIT is applicable for evaluation of olfactory function within the population of Central Russia and allows us to detect age-related differences in olfactory function. However, several odor samples presented within the original UPSIT proved to be unfamiliar to the subjects due to cultural differences. We have identified and tested odor items that may replace poorly recognizable items of the original UPSIT. Thus, we have developed a culturally adapted version of the UPSIT to be used within the population of Central Russia. 相似文献