Methylation-mediated miR-214 regulates proliferation and drug sensitivity of renal cell carcinoma cells through targeting LIVIN

LIVIN, a member of the inhibitor of apoptosis proteins (IAPs), is reported playing important roles in the development and progression of multiple human cancers. However, its underlined mechanisms in human renal cell carcinoma (RCC) are still needed to be clarified. In the present study, we reported that inhibition of miR-214 promoted the expression of LIVIN, then facilitated RCC cells growth and reduced the sensitivity of RCC cells to chemotherapeutic drugs. In constant, overexpression of miR-214 had contradictory effects. Further investigation showed that miR-214 was down-regulated in RCC because of abnormal methylation. In addition, DNA methyltransferase DNMT1, miR-214 and LIVIN are directly correlated in RCC patients. In conclusion, these results suggest that abnormal miR-214 methylation negatively regulates LIVIN, which may promote RCC cells growth and reduced the sensitivity of RCC cells to chemotherapeutic drugs.     

植物提取物对小菜蛾化蛹率和蛹重的影响

分别用 35种植物乙醇提取物处理的叶片饲养小菜蛾幼虫 ,研究非嗜食植物提取物对小菜蛾化蛹率和蛹重的影响。研究表明 ,茶枯 (Camelliaoleifera)、番石榴 (Psidiumguajava)、飞扬草 (Euphorbiahirta)、南洋楹 (Albiziafalcataria)、薄荷 (Menthahaplocalyx)、蓖麻 (Ricinuscommunis)、假连翘 (Durantarepens)的乙醇提取物对小菜蛾化蛹有显著的抑制作用 (P <0 0 1,DMRT) ,化蛹率都低于 30 0 0 % ;从蛹重来看 ,经积雪草 (Centellaasiatica)、芒果(Mangiferaindica)、构树 (Broussonetiapapyrifera)、细叶桉 (Eucalyptustereticornis)的乙醇提取物处理 ,小菜蛾蛹重显著减轻 (P <0 0 1,DMRT) ,蛹重 (10只蛹 )都低于 0 0 30 0 g。     

Circ-EGFR Functions as an Inhibitory Factor in the Malignant Progression of Glioma by Regulating the miR-183-5p/TUSC2 Axis

Cellular and Molecular Neurobiology - Circular RNAs (circRNAs) have pivotal functions in regulating diverse biological processes of human tumors, including glioma. Herein, a novel circRNA epidermal...     

Reversible ubiquitination shapes NLRC5 function and modulates NF-κB activation switch

NLRC5 is an important regulator in innate immune responses. However, the ability of NLRC5 to inhibit NF-κB activation is controversial in different cell types. How dynamic modification of NLRC5 shapes NF-κB signaling remains unknown. We demonstrated that NLRC5 undergoes robust ubiquitination by TRAF2/6 after lipopolysaccharide treatment, which leads to dissociation of the NLRC5–IκB kinase complex. Experimental and mathematical analyses revealed that the K63-linked ubiquitination of NLRC5 at lysine 1,178 generates a coherent feedforward loop to further sensitize NF-κB activation. Meanwhile, we found USP14 specifically removes the polyubiquitin chains from NLRC5 to enhance NLRC5-mediated inhibition of NF-κB signaling. Furthermore, we found that different cell types may exhibit different sensitivities to NF-κB activation in response to NLRC5 ablation, possibly as a result of the various intrinsic levels of deubiquitinases and NLRC5. This might partially reconcile controversial studies and explain why NLRC5 exhibits diverse inhibitory efficiencies. Collectively, our results provide the regulatory mechanisms of reversible NLRC5 ubiquitination and its role in the dynamic control of innate immunity.     

Tanshinone IIA and tanshinone I production by Trichoderma atroviride D16, an endophytic fungus in Salvia miltiorrhiza

In this study the isolation of an endophytic fungus from the root of the medicinal herb Salvia miltiorrhiza Bunge is reported for the first time. The fungus produced tanshinone I and tanshinone IIA in rich mycological medium (potato dextrose broth) under shake flask and bench scale fermentation conditions. The fungus was identified as Trichoderma atroviride by its morphology and authenticated by ITS analysis (ITS1 and ITS2 regions and the intervening 5.8S rDNA region). Tanshinone I and tanshinone IIA were identified by HPLC and LC-HRMS/MS and confirmed through comparison with authentic standards. This endophytic fungus has significant scientific and industrial potential to meet the pharmaceutical demands for tanshinone I and tanshinone IIA in a cost-effective, easily accessible and reproducible way.     

黄精的研究进展

在广泛文献检索基础上,从黄精的鉴别,化学成分,药理活性,临床应用,人工栽培及组织培养等方面进行了综述,为黄精的药学研究与开发利用提供科学依据。