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Shinagawa E Toyama H Matsushita K Tuitemwong P Theeragool G Adachi O 《Bioscience, biotechnology, and biochemistry》2006,70(4):850-857
Membrane-bound NADP-independent formaldehyde-oxidizing enzyme was purified to homogeneity from the membrane of Acetobacter sp. SKU 14 isolated in Thailand. The enzyme was solubilized from the membrane fraction of glycerol-grown cells with 1% Tween 20 at pH 2.85, and purified to homogeneity through the steps of column chromatographies on DEAE-Sephadex A-50 and Q-Sepharose in the presence of 0.1% Tween 20 and 0.1% Triton X-100. The enzyme purified together with a cytochrome c showed a single protein band on native-PAGE, and was dissociated into three different subunits upon SDS-PAGE with molecular masses of 78 kDa, 55 kDa, and 18 kDa. The purified enzyme was finally characterized as a quinoprotein alcohol dehydrogenase (QADH), and this is the first indication that QADH highly oxidizes formaldehyde. The substrate specificity of the enzyme was found to be broad toward aldehydes and alcohols, and alcohols, especially n-butanol, n-propanol, and ethanol, were oxidized more rapidly than formaldehyde. 相似文献
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Sakaoporn Prachantasena Petcharatt Charununtakorn Suthida Muangnoicharoen Luck Hankla Natthaporn Techawal Prapansak Chaveerach Pravate Tuitemwong Nipa Chokesajjawatee Nicola Williams Tom Humphrey Taradon Luangtongkum 《PloS one》2016,11(2)
Poultry and poultry products are commonly considered as the major vehicle of Campylobacter infection in humans worldwide. To reduce the number of human cases, the epidemiology of Campylobacter in poultry must be better understood. Therefore, the objective of the present study was to determine the distribution and genetic relatedness of Campylobacter in the Thai chicken production industry. During June to October 2012, entire broiler production processes (i.e., breeder flock, hatchery, broiler farm and slaughterhouse) of five broiler production chains were investigated chronologically. Representative isolates of C. jejuni from each production stage were characterized by flaA SVR sequencing and multilocus sequence typing (MLST). Amongst 311 selected isolates, 29 flaA SVR alleles and 17 sequence types (STs) were identified. The common clonal complexes (CCs) found in this study were CC-45, CC-353, CC-354 and CC-574. C. jejuni isolated from breeders were distantly related to those isolated from broilers and chicken carcasses, while C. jejuni isolates from the slaughterhouse environment and meat products were similar to those isolated from broiler flocks. Genotypic identification of C. jejuni in slaughterhouses indicated that broilers were the main source of Campylobacter contamination of chicken meat during processing. To effectively reduce Campylobacter in poultry meat products, control and prevention strategies should be aimed at both farm and slaughterhouse levels. 相似文献
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Mathurot Duangchanchot Rapee Inpunkaew Pravate Thongsiri Nobuhito Hayashimoto Nobuhiro Gemma Masaru Nikaido Masayoshi Takahashi Kanchana Kengkoom 《Experimental Animals》2014,63(2):169-173
Prevalence of Helicobacter is mostly unknown in laboratory animals in
Thailand. The 221 mice feces/cecum from 8 universities, 2 pharmaceutical companies and 3
research institutions in Thailand were surveyed for the prevalence and distribution of
Helicobacter species by using the Electrochemical DNA chip.
Helicobacter were detected 23/46 samples in Specific Pathogen Free
(SPF) and 168/175 in conventional condition. Prevalence of Helicobacter
were 98%, 96%, 92% and 78% in South (n=40), Northeast (n=40), North (n=25) and Central
area (n=116), respectively. Only Central area holds SPF facility resulting in
Helicobacter prevalence that seems to be lower than other areas. Three
species of Helicobacter were detected in feces/cecum samples by sequence
analysis: H. rodentium (67.0%, 148 samples),
Helicobacter sp. MIT 01-6451 (15.4%, 34 samples), and unidentified
Helicobacter species (14.1%, 9 samples). The results suggested that
H. rodentium is the most common species of
Helicobacter in laboratory mice in Thailand. 相似文献
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