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K. Petrikov Ya. Delegan A. Surin O. Ponamoreva I. Puntus A. Filonov A. Boronin 《Process Biochemistry》2013,48(5-6):931-935
We studied formation and structural features of biosurfactants produced by five oil-degrading Pseudomonas and Rhodococcus strains. These bacteria were found to be capable of intense formation of extracellular glycolipid biosurfactants when grown on mineral salts medium with 2% hexadecane. Under these conditions, the surface tension of the cultures decreased from 77 mN/m to 31–34 mN/m. The strain Rhodococcus sp. S26 forming up to 780 mg glycolipids/l of culture medium proved the most efficient biosurfactant producer. Extracellular glycolipids were purified from the crude extracts by column chromatography. Their structural features were determined by thin layer chromatography and electrospray ionization mass spectrometry. Strains Pseudomonas putida BS3701 and Pseudomonas fluorescens 142NF synthesized a number of glycolipids identified as rhamnolipid B and its homologues. Glycolipids produced by Rhodococcus sp. X5 and Rhodococcus sp. S26 were assigned to trehalose tetraesters. 相似文献
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Tarasov S. E. Plekhanova Yu. V. Kitova A. E. Bykov A. G. Machulin A. V. Kolesov V. V. Klenova N. A. Revin V. V. Ponamoreva O. N. Reshetilov A. N. 《Applied Biochemistry and Microbiology》2022,58(4):468-477
Applied Biochemistry and Microbiology - Bacterial cellulose (BC) produced by the Komagateibacter sucrofermentas VKPM B-11267 bacteria was used as a carrier for immobilization of acetic acid... 相似文献
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V. D. Rumyantseva E. A. Aksenova O. N. Ponamoreva A. F. Mironov 《Russian Journal of Bioorganic Chemistry》2000,26(6):423-428
We found that thionyl chloride can chlorinate porphyrin complexes with transient metals (Pd, Ni, or Cu) at the free β andmeso-positions of the porphyrin macrocycle. A more prolonged or rigorous treatment also causes the chlorination of side alkyl
substituents, mainly, methyl groups. 相似文献
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T. A. Kuznetsova A. P. Beschastny O. N. Ponamoreva Yu. A. Trotsenko 《Applied Biochemistry and Microbiology》2012,48(6):546-551
Methanol dehydrogenase (MDH) of the facultative methylotrophic phytosymbiont Methylobacterium nodulans has been purified for the first time to an electrophoretically homogeneous state and characterized. The native protein with a molecular mass of 70 kDa consists of large (60 kDa) and small (6.5 kDa) subunits. The purified protein displayed a spectrum identical to that of pyrroloquinoline quinone (PQQ)-containing MDH, pI 8.7, pH optimum in the range 9–10. The enzyme was inactive in the absence of ammonium or methylamine and exhibited a wide substrate specificity with regard to C1–C5 alcohols with the high-est affinity to methanol (K M = 70 μM), but it did not oxidize benzyl and secondary alcohols. The apparent K M values to primary alcohols increased with the length of the carbon chain. The enzyme was characterized by a high stability level even in the absence of a substrate. An immobilized enzyme was used for amperometric methanol detection. 相似文献
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We found that thionyl chloride can chlorinate porphyrin complexes with transient metals (Pd, Ni, or Cu) at the free beta- and meso-positions of the porphyrin macrocycle. A more prolonged or rigorous treatment also causes the chlorination of side alkyl substituents, mainly, methyl groups. 相似文献
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S. V. Alferov P. R. Minaicheva V. A. Arlyapov L. D. Asulyan V. A. Alferov O. N. Ponamoreva A. N. Reshetilov 《Applied Biochemistry and Microbiology》2014,50(6):637-643
Acetic acid bacteria Gluconobacter oxydans subsp. industrius RKM V-1280 were immobilized into a synthetic matrix based on polyvinyl alcohol modified with N-vinylpyrrolidone and used as biocatalysts for the development of bioanodes for microbial fuel cells. The immobilization method did not significantly affect bacterial substrate specificity. Bioanodes based on immobilized bacteria functioned stably for 7 days. The maximum voltage (fuel cell signal) was reached when 100–130 μM of an electron transport mediator, 2,6-dichlorophenolindophenol, was added into the anode compartment. The fuel cell signals reached a maximum at a glucose concentration higher than 6 mM. The power output of the laboratory model of a fuel cell based on the developed bioanode reached 7 mW/m2 with the use of fermentation industry wastes as fuel. 相似文献
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Arlyapov V Kamanin S Ponamoreva O Reshetilov A 《Enzyme and microbial technology》2012,50(4-5):215-220
The parameters of biosensors based on the yeast strains Candida maltosa VKM Y-2359, Candida blankii VKM Y-2675, and Debaryomyces hansenii VKM Y-2482 for biochemical oxygen demand (BOD) detection are compared. The catalytic activity of the strains was analyzed in relation to the growth phase. The possibility of using D. hansenii as a basis for receptor element of a biosensor for BOD detection in municipal and biotechnological wastewaters was shown. 相似文献
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Elena P. Ivanova Hooi Jun Ng Hayden K. Webb Valeriya V. Kurilenko Natalia V. Zhukova Valery V. Mikhailov Olga N. Ponamoreva Russell J. Crawford 《Antonie van Leeuwenhoek》2013,103(4):877-884
A non-pigmented, motile, Gram-negative bacterium designated H 17T was isolated from a seawater sample collected in Port Phillip Bay (the Tasman Sea, Pacific Ocean). The new organism displayed optimal growth between 4 and 37 °C, was found to be neutrophilic and slightly halophilic, tolerating salt water environments up to 10 % NaCl. Strain H 17T was found to be able to degrade starch and Tween 80 but unable to degrade gelatin or agar. Phosphatidylglycerol (27.7 %) and phosphatidylethanolamine (72.3 %) were found to be the only associated phospholipids. The major fatty acids identified are typical for the genus Alteromonas and include C16:0, C16:1ω7, C17:1ω8 and C18:1ω7. The G+C content of the DNA was found to be 43.4 mol%. A phylogenetic study, based on the 16S rRNA gene sequence analysis and Multilocus Phylogenetic Analysis, clearly indicated that strain H 17T belongs to the genus Alteromonas. The DNA?DNA relatedness between strain H 17T and the validly named Alteromonas species was between 30.7 and 46.4 mol%. Based on these results, a new species, Alteromonas australica, is proposed. The type strain is H 17T (= KMM 6016T = CIP 109921T). 相似文献
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O. N. Ponamoreva T. Z. Esikova Yu. A. Vlasova B. P. Baskunov V. A. Alferov 《Microbiology》2010,79(3):321-326
A biosensor based on the most active caprolactam-degrading strain Pseudomonas putida BS394(pBS268) was used in the study of aerobic degradation of linear caprolactam oligomers by bacterial cells. The changes
in the respiratory activity of the strain depend quantitatively on caprolactam dimer concentration, making it possible to
develop biosensors for detection of caprolactam oligomers in aqueous media. Based on mass spectrometry data, the scheme of
transformation of linear caprolactam oligomers by the degrader strain P. putida BS394(pBS268) was proposed for the first time. It was found that oxidative transamination to respective dicarbonic acids
may be one of the mechanisms of transformation of linear caprolactam oligomers. According to the scheme proposed, the ability
of the caprolactam-degrading strain to transform linear oligomers results from the broad substrate specificities of two enzymes
of the caprolactam degradation pathway: 2-oxoglutarate-6-aminohexanoate transaminase and 6-oxohexanoate dehydrogenase. Transformation
of linear oligomers is genetically controlled by the CAP biodegradation plasmid pBS268. 相似文献
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