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Escherichia coli grown anaerobically for osmotic studies upon increased osmolarity in alkaline medium carried out H+–K+-exchange in two steps, the first of which was DCCD1 sensitive and osmo-dependent and had the 2H+/K+ stoichiometry. H+-efflux in the presence of protonophore (CCCP) upon increase of osmolarity was shown to be high and inhibited by DCCD, whereas H+-efflux induced by a decrease of osmolarity was small and not inhibited by DCCD. The 2H+/K+-exchange was absent intrkA anduncA mutants. InuncB mutant 2H+/K+-exchange was not DCCD-and osmosensitive. Competition between DCCD and osmoshock on inhibition of 2H+/K+-exchange was found. Osmosensitivity of this exchange disappeared in spheroplasts. Osmosensitivity of both 2H+/K+-exchange and the F0F1 and osmoregulation of the F0F1 via F0 and a periplasmic space are postulated.Abbreviations F0F1
H+-ATPase complex
- F0
H+-channel, proteolipid
- F1
H+-ATPase
-
Trk
constitutive system for K+ uptake
- PV
periplasmic protein valve
- DCCD
N,N-dicyclohexylcarbodiimide
- CCCP
carbonylcyanide-m-chlorophenylhydrazone
- H or K
transmembrane electrochemical gradient for H+ or K+ respectively
-
membrane potential
- upshock or downshock
increase or decrease of medium osmolarity, respectively
- CGSC
E. coli Genetic Stock Center, Yale University, USA 相似文献
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