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排序方式: 共有419条查询结果,搜索用时 15 毫秒
1.
Carlos Rico José Luis Rico Noelia Muñoz Beatriz Gómez Iñaki Tejero 《Engineering in Life Science》2011,11(5):476-481
The effect of mixing on biogas production of a 1.5‐m3 pilot continuous stirred tank reactor (CSTR) processing screened dairy manure was evaluated. Mixing was carried out by recirculation of reactor content with a mono pump. The experiment was conducted at a controlled temperature of 37±1°C and hydraulic retention times (HRTs) of 20 and 10 days. The effect of continuous and intermittent operation of the recirculation pump on biogas production was studied. At 10 days of HRT, the results showed a minimal influence of recirculation rate on biogas production and that continuous recirculation did not improve reactor performance. At 20 days of HRT, the recirculation rate did not affect reactor performance. Combination of low solid content in feed animal slurry and long HRTs results in minimal mixing requirements for anaerobic digestion. 相似文献
2.
Sameena Khan Ankur Garg Arvind Sharma Noelia Camacho Daria Picchioni Adéla?de Saint-Léger Lluís Ribas de Pouplana Manickam Yogavel Amit Sharma 《PloS one》2013,8(6)
Specific activation of amino acids by aminoacyl-tRNA synthetases (aaRSs) is essential for maintaining fidelity during protein translation. Here, we present crystal structure of malaria parasite Plasmodium falciparum tryptophanyl-tRNA synthetase (Pf-WRS) catalytic domain (AAD) at 2.6 Å resolution in complex with L-tryptophan. Confocal microscopy-based localization data suggest cytoplasmic residency of this protein. Pf-WRS has an unusual N-terminal extension of AlaX-like domain (AXD) along with linker regions which together seem vital for enzymatic activity and tRNA binding. Pf-WRS is not proteolytically processed in the parasites and therefore AXD likely provides tRNA binding capability rather than editing activity. The N-terminal domain containing AXD and linker region is monomeric and would result in an unusual overall architecture for Pf-WRS where the dimeric catalytic domains have monomeric AXDs on either side. Our PDB-wide comparative analyses of 47 WRS crystal structures also provide new mechanistic insights into this enzyme family in context conserved KMSKS loop conformations. 相似文献
3.
Expression of canonical SOS genes is not under LexA repression in Bdellovibrio bacteriovorus 下载免费PDF全文
The here-reported identification of the LexA-binding sequence of Bdellovibrio bacteriovorus, a bacterial predator belonging to the delta-Proteobacteria, has made possible a detailed study of its LexA regulatory network. Surprisingly, only the lexA gene and a multiple gene cassette including dinP and dnaE homologues are regulated by the LexA protein in this bacterium. In vivo expression analyses have confirmed that this gene cassette indeed forms a polycistronic unit that, like the lexA gene, is DNA damage inducible in B. bacteriovorus. Conversely, genes such as recA, uvrA, ruvCAB, and ssb, which constitute the canonical core of the Proteobacteria SOS system, are not repressed by the LexA protein in this organism, hinting at a persistent selective pressure to maintain both the lexA gene and its regulation on the reported multiple gene cassette. In turn, in vitro experiments show that the B. bacteriovorus LexA-binding sequence is not recognized by other delta-Proteobacteria LexA proteins but binds to the cyanobacterial LexA repressor. This places B. bacteriovorus LexA at the base of the delta-Proteobacteria LexA family, revealing a high degree of conservation in the LexA regulatory sequence prior to the diversification and specialization seen in deeper groups of the Proteobacteria phylum. 相似文献
4.
Noelia Lander Miguel A. Chiurillo Roberto Docampo 《The Journal of eukaryotic microbiology》2016,63(5):679-690
Genome editing by CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 (CRISPR‐associated gene 9) system has been transformative in biology. Originally discovered as an adaptive prokaryotic immune system, CRISPR/Cas9 has been repurposed for genome editing in a broad range of model organisms, from yeast to mammalian cells. Protist parasites are unicellular organisms producing important human diseases that affect millions of people around the world. For many of these diseases, such as malaria, Chagas disease, leishmaniasis and cryptosporidiosis, there are no effective treatments or vaccines available. The recent adaptation of the CRISPR/Cas9 technology to several protist models will be playing a key role in the functional study of their proteins, in the characterization of their metabolic pathways, and in the understanding of their biology, and will facilitate the search for new chemotherapeutic targets. In this work we review recent studies where the CRISPR/Cas9 system was adapted to protist parasites, particularly to Apicomplexans and trypanosomatids, emphasizing the different molecular strategies used for genome editing of each organism, as well as their advantages. We also discuss the potential usefulness of this technology in the green alga Chlamydomonas reinhardtii. 相似文献
5.
Recent Findings in the Chemistry of Odorants from Four Baccharis Species and Their Impact as Chemical Markers 下载免费PDF全文
Manuel Minteguiaga Noelia Umpiérrez Vanessa Xavier Aline Lucas Claudio Mondin Laura Fariña Eduardo Cassel Eduardo Dellacassa 《化学与生物多样性》2015,12(9):1339-1348
Baccharis is a widespread genus belonging to the Asteraceae family that includes almost 400 species exclusively from the Americas. Even when studied in detail, the taxonomic classification among species from this genus is not yet fully defined. Within the framework of our study of the volatile composition of the Baccharis genus, four species (B. trimera, B. milleflora, B. tridentata, and B. uncinella) were collected from the ‘Campos de Cima da Serra’ highlands of the Brazilian state of Rio Grande do Sul. The aerial parts were dried and extracted by the simultaneous distillation extraction (SDE) procedure. This is the first time that SDE has been applied to obtain and compare the volatile‐extract composition in the Baccharis genus. Characterization of the volatile extracts allowed the identification of 180 peaks with many coeluting components; these latter being detailed for the first time for this genus. The multivariate statistical analyses allowed separating the volatile extracts of the four populations of Baccharis into two separate groups. The first one included the B. milleflora, B. trimera, and B. uncinella volatile extracts. The three species showed a high degree of similarity in their volatile composition, which was characterized by the presence of high contents of sesquiterpene compounds, in particular of spathulenol. The second group comprised the extract of B. tridentata, which contained α‐pinene, β‐pinene, limonene, and (E)‐β‐ocimene in high amounts. 相似文献
6.
The chitinivorous ciliate Ascophrys, an ectosymbiont of the shrimp Palaemon serratus, is enclosed by a thick cyst wall except for a ventral hiatus exposing a circular area of exoskeleton to the interior of the cyst. The exoskeleton underlying the cyst wall remains intact, but the circular area of exoskeleton is dissolved enzymatically and ingested. The feeding ciliate forms a cavity in the exoskeleton into which it sinks. Its complex oral apparatus resembles a pump encircled by cytoplasm containing Golgi and high concentrations of coated vesicles that join pellicular pores between cilia. The ingestive apparatus is formed of microtubular lamellae that originate in the midplane of the body, descend toward a coated membrane on the surface, and ascend again as a lamellar lining to a complex food tube that ends in the middle of the body surrounded by food vacuoles. The cytoplasm enclosed between the descending lamellae and the food tube is crowded with membrane organelles that recycle as food vacuole membranes at the coated membrane. We hypothesize that vacuoles containing dissolved exoskeleton are drawn up into the oral tube and are released into the cytoplasm at the terminus of the tube, where their contents are concentrated and excess vacuolar membrane collapsed into membrane organelles. 相似文献
7.
Structural organization and a standardized nomenclature for plant endo-1,4-beta-glucanases (cellulases) of glycosyl hydrolase family 9 下载免费PDF全文
8.
García-Vaquero MP Moreside JM Brontons-Gil E Peco-González N Vera-Garcia FJ 《Journal of electromyography and kinesiology》2012,22(3):398-406
The aim of this study was to analyze trunk muscle activity during bridge style stabilization exercises, when combined with single and double leg support strategies. Twenty-nine healthy volunteers performed bridge exercises in 3 different positions (back, front and side bridges), with and without an elevated leg, and a quadruped exercise with contralateral arm and leg raise ("bird-dog"). Surface EMG was bilaterally recorded from rectus abdominis (RA), external and internal oblique (EO, IO), and erector spinae (ES). Back, front and side bridges primarily activated the ES (approximately 17% MVC), RA (approximately 30% MVC) and muscles required to support the lateral moment (mostly obliques), respectively. Compared with conventional bridge exercises, single leg support produced higher levels of trunk activation, predominantly in the oblique muscles. The bird-dog exercise produced greatest activity in IO on the side of the elevated arm and in the contralateral ES. In conclusion, during a common bridge with double leg support, the antigravity muscles were the most active. When performed with an elevated leg, however, rotation torques increased the activation of the trunk rotators, especially IO. This information may be useful for clinicians and rehabilitation specialists in determining appropriate exercise progression for the trunk stabilizers. 相似文献
9.
Estaras Matias Ortiz-Placin Candido Castillejo-Rufo Alba Fernandez-Bermejo Miguel Blanco Gerardo Mateos Jose M. Vara Daniel Gonzalez-Cordero Pedro L. Chamizo Sandra Lopez Diego Rojas Adela Jaen Isabel de Armas Noelia Salido Gines M. Iovanna Juan L. Santofimia-Castaño Patricia Gonzalez Antonio 《Journal of physiology and biochemistry》2023,79(1):235-249
Journal of Physiology and Biochemistry - We have investigated the effects of melatonin on major pathways related with cellular proliferation and energetic metabolism in pancreatic stellate cells.... 相似文献
10.