Impact of land‐use change to Jatropha bioenergy plantations on biomass and soil carbon stocks: a field study in Mali

Small‐scale Jatropha cultivation and biodiesel production have the potential of contributing to local development, energy security, and greenhouse gas (GHG) mitigation. In recent years however, the GHG mitigation potential of biofuel crops is heavily disputed due to the occurrence of a carbon debt, caused by CO₂ emissions from biomass and soil after land‐use change (LUC). Most published carbon footprint studies of Jatropha report modeled results based on a very limited database. In particular, little empirical data exist on the effects of Jatropha on biomass and soil C stocks. In this study, we used field data to quantify these C pools in three land uses in Mali, that is, Jatropha plantations, annual cropland, and fallow land, to estimate both the Jatropha C debt and its C sequestration potential. Four‐year‐old Jatropha plantations hold on average 2.3 Mg C ha^?1 in their above‐ and belowground woody biomass, which is considerably lower compared to results from other regions. This can be explained by the adverse growing conditions and poor local management. No significant soil organic carbon (SOC) sequestration could be demonstrated after 4 years of cultivation. While the conversion of cropland to Jatropha does not entail significant C losses, the replacement of fallow land results in an average C debt of 34.7 Mg C ha^?1, mainly caused by biomass removal (73%). Retaining native savannah woodland trees on the field during LUC and improved crop management focusing on SOC conservation can play an important role in reducing Jatropha's C debt. Although planting Jatropha on degraded, carbon‐poor cropland results in a limited C debt, the low biomass production, and seed yield attained on these lands reduce Jatropha's potential to sequester C and replace fossil fuels. Therefore, future research should mainly focus on increasing Jatropha's crop productivity in these degraded lands.     

Restoration of Dry Afromontane Forest Using Pioneer Shrubs as Nurse-Plants for Olea europaea ssp. cuspidata

Shrubs are often considered competitive barriers for seedlings planted in reforestation programs, although they can facilitate tree recruitment, especially in ecosystems under high abiotic stress. An alternative reforestation technique using pioneer shrubs as nurse‐plants for Olea europaea ssp. cuspidata was tested in exclosures in northern Ethiopia. Seedlings were planted in three different microhabitats, and their survival was monitored. The microhabitats were bare soil patches between shrubs, patches under the dominant shrub Acacia etbaica, and patches under Euclea racemosa, an evergreen shrub, which supports the majority of naturally established Olea recruits. The ability of shrubs to offer protection against browsing goats was tested experimentally. Controlled shading was used to determine whether solar irradiation causes seedling mortality in environments without water stress. Data were analyzed using Kaplan–Meier survival analysis, Kruskal–Wallis analysis of variance (ANOVA), and one‐way ANOVA. Olea survival was significantly higher and shoot damage by goats was lower when planted under shrub cover compared to bare soil patches, particularly under Euclea canopies, although high shade levels reduced seedling performance. Reduction of solar radiation by shrub canopies and thus control of soil–water evaporation and seedling transpiration most likely controlled the observed facilitation. Planting under shrubs may increase seedling survival and assist regeneration of dry Afromontane vegetation. Preserving pioneers also reduces soil erosion and conserves biodiversity. Excluding livestock is essential for Olea woodland restoration and allows persistent but morphologically modified Olea shrubs to develop vigorous regrowth. Facilitative processes are guiding principles for assisted forest restoration, but above‐average rains may be critical to restore higher biomass levels in semiarid areas.     

Characterization of testudine melanomacrophage linear,membrane extension processes—Cablepodia—By phase and atomic force microscopy

Summary Melanomacrophages (MMs) are a component of an internal, pigmented cell system in liver and splenic tissues of some fishes, anurans, and reptiles. The cells have been found in centers or aggregates in sinusoids and are associated with cells capable of producing a peptide cytokine and immunoglobulins. A unique cell extension process has been observed in turtle MMs placed into cell culture, and this process has been studied by light and atomic force microscopy. These structures, referred to as cablepodia, are uniquely straight, narrow, and unbranching and appear to originate from growth cones opposite lamellipodia. Cablepodia were found to connect with other turtle MMs and fibroblasts forming cell networks. Dividing fibroblasts to which a cablepodium attached ceased cell division. The observations collectively suggest that a principal reason for aggregations of MMs in internal organs of lower vertebrates in their ability to form interconnected networks of cell processes for trapping and processing of particulate matter, cells and infectious organisms and, possibly, for the communication of cell signals and transfer of intracellular materials.     

Effect of tree species choice and mineral capping in a woodland phytostabilisation system: A case-study for calcareous dredged sediment landfills with an oxidised topsoil

Foliar, wood and bark metal concentrations were evaluated 2 and 12 years after afforestation of 3 metal-polluted calcareous upland dredged sediment landfills with an oxidised topsoil. Two techniques for reducing the metal entry into the food web were compared in a field situation: choice of appropriate tree species aiming at reduced metal transfer to the environment, and application of a thin uncontaminated mineral capping layer on top of the polluted sediment aiming at a good topsoil quality. Foliar concentrations show that an appropriate choice of tree species is very important in controlling metal cycling. Common ash, sycamore maple, pedunculate oak, black alder and small-leaved lime had normal foliar metal concentrations, while hybrid poplar showed very high Cd and Zn concentrations. The absence of a thin uncontaminated covering layer resulted in a higher metal load of the litter through direct (surface soil adhesion) and indirect (raindrop splash) soil load of the litter layer. Mineral capping affected foliar concentrations in an element-specific and species-specific way. It is concluded that an uncontaminated covering layer on polluted soils before afforestation is a management option which is only efficient in case of an appropriate tree species choice.     

Root tensile strength and root distribution of typical Mediterranean plant species and their contribution to soil shear strength

In Mediterranean environments, gully erosion is responsible for large soil losses. It has since long been recognized that slopes under vegetation are much more resistant to soil erosion processes compared to bare soils and improve slope stability. Planting or preserving vegetation in areas vulnerable to erosion is therefore considered to be a very effective soil erosion control measure. Re-vegetation strategies for erosion control rely in most cases on the effects of the above-ground biomass in reducing water erosion rates, whereas the role of the below-ground biomass is often neglected or underestimated. While the above-ground biomass can temporally disappear in semi-arid environments, roots may still be present underground and play an important role in protecting the topsoil from being eroded. In order to evaluate the potential of plant species growing in Mediterranean environments to prevent shallow mass movements on gully or terrace walls, the root reinforcement effect of 25 typical Mediterranean matorral species (i.e. shrubs, grasses herbs, small trees) was assessed, using the simple perpendicular model of Wu et al. (Can Geotech J 16:19–33, 1979). As little information is available on Mediterranean plant root characteristics, root distribution data were collected in SE-Spain and root tensile strength tests were conducted in the laboratory. The power root tensile strength–root diameter relationships depend on plant species. The results show that the shrubs Salsola genistoides Juss. Ex Poir. and Atriplex halimus L. have the strongest roots, followed by the grass Brachypodium retusum (Pers.) Beauv. The shrubs Nerium oleander L. and the grass Avenula bromoides (Gouan) H. Scholz have the weakest roots in tension. Root area ratio for the 0–0.1 m topsoil ranges from 0.08% for the grass Piptatherum miliaceum (L.) Coss to 0.8% for the tree Tamarix canariensis Willd. The rush Juncus acutus L. provides the maximum soil reinforcement to the topsoil by its roots (i.e. 304 kPa). Grasses also increase soil shear strength significantly (up to 244 kPa in the 0–0.1 m topsoil for Brachypodium retusum (Pers.) Beauv.). The shrubs Retama sphaerocarpa (L.) Boiss. and Anthyllis cytisoides L. are increasing soil shear strength to a large extent as well (up to 134 and 160 kPa respectively in the 0–0.10 m topsoil). Whereas grasses and the rush Juncus acutus L. increase soil shear strength in the topsoil (0–0.10 m) to a large extent, the shrubs Anthyllis cytisoides (L.), Retama sphaerocarpa (L.) Boiss., Salsola genistoides Juss. Ex Poir. and Atriplex halimus L. strongly reinforce the soil to a greater depth (0–0.5 m). As other studies reported that Wu’s model overestimates root cohesion values, reported root cohesion values in this study are maximum values. Nevertheless, the calculated cohesion values are used to rank species according to their potential to reinforce the soil.     

The effect of drought stress on heterozygosity–fitness correlations in pedunculate oak (Quercus robur)

Background and Aims

The interaction between forest fragmentation and predicted climate change may pose a serious threat to tree populations. In small and spatially isolated forest fragments, increased homozygosity may directly affect individual tree fitness through the expression of deleterious alleles. Climate change-induced drought stress may exacerbate these detrimental genetic consequences of forest fragmentation, as the fitness response to low levels of individual heterozygosity is generally thought to be stronger under environmental stress than under optimal conditions.

Methods

To test this hypothesis, a greenhouse experiment was performed in which various transpiration and growth traits of 6-month-old seedlings of Quercus robur differing in multilocus heterozygosity (MLH) were recorded for 3 months under a well-watered and a drought stress treatment. Heterozygosity–fitness correlations (HFC) were examined by correlating the recorded traits of individual seedlings to their MLH and by studying their response to drought stress.

Key Results

Weak, but significant, effects of MLH on several fitness traits were obtained, which were stronger for transpiration variables than for the recorded growth traits. High atmospheric stress (measured as vapour pressure deficit) influenced the strength of the HFCs of the transpiration variables, whereas only a limited effect of the irrigation treatment on the HFCs was observed.

Conclusions

Under ongoing climate change, increased atmospheric stress in the future may strengthen the negative fitness responses of trees to low MLH. This indicates the necessity to maximize individual multilocus heterozygosity in forest tree breeding programmes.     

Carbon and Water Footprints and Energy Use of Greenhouse Tomato Production in Northern Italy

This study reports on the carbon, water, and energy footprints of tomatoes grown in a greenhouse in Northern Italy and two possible future variations of heating and carbon dioxide (CO₂) fertilization on the current setup. The heat supply in place, consisting of natural gas (NG) and canola oil combustion, is compared to cogeneration and incineration of municipal solid waste for heating and CO₂ from industrial exhaust for fertilization. As a benchmark, the current system is also compared to a conventional system, in which heat is delivered solely based on NG. Each kilogram (kg) of fresh tomatoes (“Cuore di Bue” variety) produced in the current greenhouse emits 2.28 kg CO₂ equivalents (eq) and uses 95.5 megajoules (MJ) eq energy and 122 liters (L) of water. Relative to the system in place, the carbon footprint (CF) is 57.5% and 18% higher with conventional NG heating and cogeneration and is 40% lower with waste valorization. Further, 33%, 55%, and 63% less energy and 9%, 96%, and 14% less water are used in the conventional, cogeneration, and waste valorization scenarios, respectively. This confirms that there are multiple strategies to reduce the impact of the tomato production under consideration.     

A quantitative indicator framework for stand level evaluation and monitoring of environmentally sustainable forest management

An indicator framework was designed as an operational science-based tool for the evaluation of the environmental aspects of sustainable forest management at stand level in Flanders (Belgium). The framework aims to assess the effects of forest management on forest composition, structure and functioning. It consists of seven principles and 19 criteria, to which 157 potential indicators, selected from literature, were assigned; 40 of these were considered as suitable by an expert panel, based on 10 operational selection criteria. All indicators were quantitative variables measurable in the field.After elaboration of a measurement protocol, the indicator framework was validated in 115 forest stands, distributed over the three main forest types of Flanders. The new indicator framework exhibited greater sensitivity to forest management practices and demonstrated better discriminating power than the method that is currently used by the Flemish forest administration to estimate the naturalness and environmental quality of a forest stand. Following a detailed cost calculation of each indicator and based on the sensitivity of each indicator to forest management practices, the indicator framework was further reduced to a final set of 29 indicators. This framework can also be applied in other regions, provided that local threshold values are defined to convert indicator values to indicator scores.The selection procedure and the possible contribution of this set to the forest management in Flanders are discussed.     

Uroporphyria development in cultured chick embryo fibroblasts long-term treated with chloramphenicol and ethidium bromide

In brain cortex, low concentrations of GTP or Gpp(NH)p activated the membrane-bound low Km cyclic AMP phosphodiesterase while higher concentrations of GTP, but not of Gpp(NH)p, reversed this activation. The adenosine analog N6-phenylisopropyladenosine (N6-PIA) elicited biphasic effect on this enzyme (activation up to 10(-8) M, complete reversion at 10(-5) M), provided that GTP was present. N6-PIA activation was reduced in the presence of Gpp(NH)p and blocked by sodium (80 mM). In contrast, the soluble low Km cyclic AMP phosphodiesterase was insensitive to GTP or N6-PIA. This study suggests that guanine nucleotides and N6-PIA exert their effects on the membrane-bound enzyme through guanine nucleotide regulatory proteins.