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F. Dhainaut N. Bihoreau J. L. Meterreau J. Lirochon R. Vincentelli G. Mignot 《Cytotechnology》1992,10(1):33-41
The performance of a protein-free medium was compared in culture flasks with a serum-supplemented medium and with a serum free medium in terms of cell growth and monoclonal antibody production by a murine hybridoma. We present results of continuous production in hollow fiber culture systems using serum-free medium and protein-free medium. In protein-free medium, it has been possible to produce large quantities of monoclonal antibody with a productivity similar to that obtained in serum-free medium. After a two steps purification process, monoclonal antibodies were characterized by SDS-PAGE, High Performance Size Exclusion Chromatography and Free Solution Capillary Electrophoresis. SDS-PAGE and high performance chromatography analysis have showed that purified monoclonal antibodies produced in serum-free medium or protein-free medium were similar. Furthermore, Capillary Electrophoresis characterization revealed that both MAbs were constituted by three isoforms with equivalent electrophoretic mobilities.Abbreviations CHES
2-(N-Cyclohexylamino)ethane-sulfonic acid
- ECS
Extracapillary Space
- FSCE
Free Solution Capillary Electrophoresis
- HPSEC
High Performance Size Exclusion Chromatography
- ICS
Intracapillary Space
- MAb
Monoclonal Antibody
- PFM
Protein-Free Medium
- SFM
Serum-Free Medium
- SSM
Serum-Supplemented Medium 相似文献
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Pennec Flora Gérard Caroline Meterreau Laura Monghiemo Claude Ngawolo Jean-Christophe Bokika Laurent Romain Narat Victor 《International journal of primatology》2020,41(6):775-799
International Journal of Primatology - Understanding the ecological and behavioral variation of primates is central to improving conservation strategies. Studies of chimpanzees (Pan troglodytes)... 相似文献
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