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Masahiko Maekawa Megumu Munakata Takayoshi Kuroda-Sowa Koji Hachiya 《Inorganica chimica acta》1995,230(1-2):249-252
A novel hexanickel(II) complex [Ni6(NCCHCH2CH2CHCN)6] (2) with 1,4-dicyanobutane-1,4-diyl (L) which was produced by the metal-induced dimerization of acrylonitrile (AN) has been isolated and the structure has been determined crystallographically. Complex 2 is triclinic, space group . Each nickel atom is coordinated by two carbon atoms of L and two nitrogen atoms of the cyano group of two other L, providing a square-plenar geometry. The six nickel atoms are bridged by the cyano group and carbon atom to form the slightly distorted octahedral Ni6 core. 相似文献
3.
Tomas Urbaitis Giedrius Gasiunas Joshua K Young Zhenglin Hou Sushmitha Paulraj Egle Godliauskaite Mantvyda M Juskeviciene Migle Stitilyte Monika Jasnauskaite Megumu Mabuchi G Brett Robb Virginijus Siksnys 《EMBO reports》2022,23(12)
Most CRISPR‐type V nucleases are stimulated to cleave double‐stranded (ds) DNA targets by a T‐rich PAM, which restricts their targeting range. Here, we identify and characterize a new family of type V RNA‐guided nuclease, Cas12l, that exclusively recognizes a C‐rich (5''‐CCY‐3′) PAM. The organization of genes within its CRISPR locus is similar to type II‐B CRISPR‐Cas9 systems, but both sequence analysis and functional studies establish it as a new family of type V effector. Biochemical experiments show that Cas12l nucleases function optimally between 37 and 52°C, depending on the ortholog, and preferentially cut supercoiled DNA. Like other type V nucleases, it exhibits collateral nonspecific ssDNA and ssRNA cleavage activity that is triggered by ssDNA or dsDNA target recognition. Finally, we show that one family member, Asp2Cas12l, functions in a heterologous cellular environment, altogether, suggesting that this new group of CRISPR‐associated nucleases may be harnessed as genome editing reagents. 相似文献
4.
Nosaka AY Kanaori K Teno N Togame H Inaoka T Takai M Kokubo T 《Bioorganic & medicinal chemistry》1999,7(2):375-379
Cathepsins K and L are cysteine proteinases which are considered to play an important role in bone resorption. Type I collagen is the most abundant component of the extracellular matrix of bone and regarded as an endogenous substrate for the cysteine proteinases in osteoclastic bone resorption. We have synthesized a fragment of Type I collagen (alpha-1) (157-192) as a substrate for the cathepsins and found that cathepsins K and L cleave the fragment at different specific sites. The major cleavage sites for cathepsin K were Met159-Gly160, Ser162-Gly163 and Arg165-Gly166, while those for cathepsin L were Gly166-Leu167 and Gln180-Gly181. The structure of the fragment was analyzed in aqueous solution by circular dichroism and proton NMR spectroscopy and the difference in the molecular recognition of collagen by cathepsins K and L was discussed from the structural aspect. 相似文献
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6.
Tooru Iwamoto Megumu Inaoka Hideyuki Naka 《Bioscience, biotechnology, and biochemistry》2013,77(8):1913-1915
Formation of transfer products from soybean arabinogalactan and glycerol by endo-1,4-β-d-galactanase from Penicillium citrinum was described. The amount of transfer products depended on the glycerol concentration. About 50% of the galactose residues which could be liberated from the polysaccharide by the enzyme were transferred to glycerol at an acceptor concentration of 2.5% (w/v). Transfer products with various polymerization degrees were accumulated at the beginning of the reaction and then those with higher polymerization degrees were degraded gradually. At a final stage of the reaction, two transfer products in addition to two hydrolysis products (galactose and galactobiose) were mainly accumulated. The two transfer products were isolated and their structures were examined. They were 2-O-β-d-galactosyl glycerol and O-β-d-galactosyl-(1 → 4)-O-β-d-galactosyl-(1 → 2)glycerol. 相似文献
7.
Monotherapy with a novel intervenolin derivative,AS‐1934, is an effective treatment for Helicobacter pylori infection 下载免费PDF全文
8.
Lettuce is a commercially important leafy vegetable that is cultivated worldwide, and it is also a target crop for plant factories.
In this study, lettuce was selected as an alternative platform for recombinant miraculin production because of its fast growth,
agronomic value, and wide availability. The taste-modifying protein miraculin is a glycoprotein extracted from the red berries
of the West African native shrub Richadella dulcifica. Because of its limited natural availability, many attempts have been made to produce this protein in suitable alternative
hosts. We produced transgenic lettuce with miraculin gene driven either by the ubiquitin promoter/terminator cassette from
lettuce or a 35S promoter/nos terminator cassette. Miraculin gene expression and miraculin accumulation in both cassettes
were compared by quantitative real-time PCR analysis, Western blotting, and enzyme-linked immunosorbent assay. The expression
level of the miraculin gene and protein in transgenic lettuce was higher and more genetically stable in the ubiquitin promoter/terminator
cassette than in the 35S promoter/nos terminator cassette. These results demonstrated that the ubiquitin promoter/terminator
cassette is an efficient platform for the genetically stable expression of the miraculin protein in lettuce and hence this
platform is of benefit for recombinant miraculin production on a commercial scale. 相似文献
9.
Takashi Inaoka Keitarou Kimura Kazuya Morimatsu Kazutaka Yamamoto 《Bioscience, biotechnology, and biochemistry》2017,81(6):1235-1240
High hydrostatic pressure (HHP) affects various cellular processes. Using a sporulation-deficient Bacillus subtilis strain, we characterized the properties of vegetative cells subjected to HHP. When stationary-phase cells were exposed to 250 MPa of HHP for 10 min at 25 °C, approximately 50% of cells were viable, although they exhibited a prolonged growth lag. The HHP-injured cells autolyzed in the presence of NaCl or KCl (at concentrations ≥100 mM). Superoxide dismutase slightly protected the viability of HHP-treated cells, whereas vegetative catalases had no effect. Thus, unlike HHP-injured Escherichia coli, oxidative stress only slightly affected vegetative B. subtilis subjected to HHP. 相似文献
10.
Atsuhiro Nabei Takayoshi Kuroda-Sowa Takashi Okubo Masahiko Maekawa Megumu Munakata 《Inorganica chimica acta》2008,361(12-13):3489-3493
Two new one-dimensional Fe(II)-bis-Schiff base complexes, [Fe(L1)(pyz)] · CH2Cl2 (1) and [Fe(L2)(pyz)] · 2CH2Cl2 (2) (H2L1 = bis(O-vanillin)-O-phenylenediimine, H2L2 = bis(O-vanillin)-2,3-naphthalenediimine, pyz = pyrazine) are reported with their crystal structures and magnetic property. Compound 1 shows a two-step SCO behavior while 2 shows HS at all the temperature range measured. Although the extension of aromatic moiety from benzene (L1) to naphthalene (L2) was introduced for the purpose of strengthening the cooperativity, it leads to the absence of SCO, due to the unanticipated π–π interaction, which leads to the longer Fe–N bond lengths and a weak ligand field around Fe(II) ion. 相似文献