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1.
M Soler-López L Malinina J Liu T Huynh-Dinh J A Subirana 《The Journal of biological chemistry》1999,274(34):23683-23686
A detailed picture of hydration and counterion location in the B-DNA duplex d(GCGAATTCG) is presented. Detailed data have been obtained by single crystal x-ray diffraction at atomic resolution (0.89 A) in the presence of Mg(2+). The latter is the highest resolution ever obtained for a B-DNA oligonucleotide. Minor groove hydration is compared with that found in the Na(+) and Ca(2+) crystal forms of the related dodecamer d(CGCGAATTCGCG). High resolution data (1.45 A) of the Ca(2+) form obtained in our laboratory are used for that purpose. The central GAATTC has a very stable hydration spine identical in all cases, independent of duplex length and crystallization conditions (counterions, space group). However, the organization of the water molecules (tertiary and quaternary layers) associated with the central spine vary in each case. 相似文献
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L V Malinina V A Tereshko V V Makhaldiani V F Zarytova E M Ivanova 《Molekuliarnaia biologiia》1987,21(2):529-542
A set of experimental phase diagrams revealing the region of existence of microcrystals in mixture "(pGpT)n.(pApC)n+spermine", n = 2,3,4, was obtained. All diagrams are wedge-like with the slope of the upper branch and the level of the lower one depending on the oligonucleotidd length. The presence of MPD, MgCl2 and NaCl changes the form of the diagrams in a different manner. A model explaining the peculiar features of the diagrams for mixture "oligonucleotide duplex+spermine" is proposed. The analysis of the diagrams was carried out on the basis of this model and the values of the binding constants for binding of spermine and Mg2+ to duplexes were estimated. Some conclusions about the types of complexes, which may form microcrystals in different regions of diagrams were made. 相似文献
4.
L V Malinina V A Tereshko A A Vagin E M Ivanova K G Skriabin 《Molekuliarnaia biologiia》1987,21(4):1142-1147
The DNA-cro-repressor complex crystals have been obtained, five DNA fragments of the same nucleotide sequence and different length being used. The rotation function for crystals of complexes with hexamer (pGpT)3 . (pApC)3 and with octamer (pGpT)3 . (pApC)3 have been calculated. The order of cro-DNA complex crystals is shown to vary with DNA length, the crystal of the complex with octamer being the most perfect among all investigated complexes. 相似文献
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We present the structure of the decanucleotide d(CGTATATACG) determined by single crystal X-ray diffraction at 1.58 A resolution. A netropsin drug is found in the minor groove with guanine stacked on a pyrrole ring of the drug, a feature described here for the first time. The stacked guanine is an extra-helical base coming from the end of a neighbour oligonucleotide. This observation may open the way to the development of minor groove binding drugs with a higher sequence selectivity. The oligonucleotide is in the B-conformation, but the terminal base-pairs are disrupted: the cytosine residues are disordered while the guanine residues penetrate into the minor groove of neighbouring duplexes. Four hydrated Ni ions with octahedral co-ordination are found associated with the N7 atoms of each guanine. The high affinity of these ions with guanine suggests that they may be used as probes for specific guanine residues. 相似文献
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Structure of the oligonucleotide d(CGTATATACG) as a site-specific complex with nickel ions 下载免费PDF全文
Abrescia NG Malinina L Fernandez LG Huynh-Dinh T Neidle S Subirana JA 《Nucleic acids research》1999,27(7):1593-1599
In this paper we explore the application of Ni2+to the crystallization of oligonucleotides. We have determined in this way the structure of a fully alternating (Y-R) decanucleotide d(CGTATATACG) by single crystal X-ray diffraction. This is the first oligonucleotide crystal structure with an alternating 5'-(TA)3-3' central part. Alternating oligonucleotides have a particular interest since they often have a unique structure. In this case the general conformation is B-like with an alternating twist and an end-to-end interaction which involves terminal guanines. The crystal belongs to space group P41212 with a = b = 52.46, c = 101.49 A. This packing imposes a 90 degrees crossing of the symmetry related helices. This is a new way of packing for decamers.The oligonucleotide structure is characterized by the specific association with seven nickel ions, involving the N7 atom of every guanine. One of the Ni2+ions is shared between two guanines of symmetry related molecules. Until now no oligonucleotide has been crystallized in the presence of this metal ion. A novel C.A.T triplet structure has also been tentatively identified. 相似文献
9.
Malinina IuA Somov AIu 《Radiatsionnaia biologiia, radioecologiia / Rossi?skaia akademiia nauk》2003,43(5):552-554
The influence of electromagnetic radiation of industrial frequency on survival, efficiency, physiological parameters of Daphnia magna (Straus) was investigated. The electromagnetic field with intensity of a magnetic component 15 microTl and an electrical one 12 kV/m was generated. The researches were carried out in acute and chronic experiments. The experimental group was exposed to electromagnetic radiation for 8 hours daily for 30 days. As a result of the study it was revealed that the influence of electromagnetic radiation with the given parameters did not have the lethal effect on the test objects. It was found that electromagnetic radiation negatively affected Daphnia fertility. On the average, a real fertility of the Daphnia in the experimental unit was 26.32% compared to the control. The age of puberty in the experiment increased twice in comparison with the control. 相似文献
10.
Results are presented from experimental and theoretical studies of the optical characteristics and parameters of the plasma
of an atmospheric-pressure barrier discharge excited in a HgBr2: N2: He mixture, which was used as the working medium of a small-size (with a radiation area of 8 cm2) exciplex gas-discharge radiation source. The mean radiation power of 87 mW was achieved at the radiation wavelength λmax = 502 nm. The electron energy distribution function, the transport characteristics, the specific energy lost in the processes
involving electrons, the electron temperature and density, and the rate constants of elastic and inelastic electron scattering
by the components of the working mixture were calculated as functions of the reduced field E/N. The plasma of a discharge excited in a HgBr2: N2: He mixture can be used as the working medium of a small-size blue-green radiation source. Such a source can find application
in biotechnology, photonics, and medicine and can also be used to manufacture gas-discharge display panels. 相似文献