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排序方式: 共有112条查询结果,搜索用时 15 毫秒
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ADELINA D. RAMIREZ ELIANA M. M. ROCHA ANTONIANA U. KRETTLI 《The Journal of eukaryotic microbiology》1995,42(6):705-708
ABSTRACT. A correlation was observed between in vivo and in vitro activity of six monoclonal antibodies (mAb) against the major circumsporozoite protein of the avian malaria Plasmodium gallinaceum as follows. (1) Two mAb were protective, totally abrogating sporozoite infectivity to chicks, its natural host, in vivo; they caused 100% inhibition of sporozoite invasion (ISI) in vitro to SL-29 chicken fibroblasts and intense ISI to cultured chicken macrophages, as well as inhibited the exoerythrocytic development of sporozoites taken up by macrophages, the initial cell host of P. gallinaceum sporozoites. (2) Two mAb were partially protective in that they reduced sporozoite infectivity to chicks, caused partial ISI to SL-29 and macrophage cells and partial inhibition to the exoerythrocytic development of sporozoites in macrophages in vitro. (3) Two mAb were totally inactive in vivo although they both bound to the sporozoite antigens as detected by indirect immunofluorescence, western blot, and ELISA; they both failed to induce ISI or inhibit the exoerythrocytic development in macrophages. The possible participation of macrophages as the initial cell type involved in sporozoite destruction in the presence of anti-circumsporozoite antibodies is discussed. 相似文献
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ELIANA M. M. ROCHA MICHAEL R. HOLLINGDALE BARBARA SINA PAMELA LELAND JOSÉ D. LOPES ANTONIANA U. KRETTLI 《The Journal of eukaryotic microbiology》1993,40(1):61-63
ABSTRACT. Monoclonal antibodies that react with the circumsporozoite protein of the avian malaria Plasmodium gallinaceum sporozoites also reacted with circumsporozoite protein of the rodent malaria Plasmodium berghei. Two types of reactivity were identified: 1) two monoclonal antibodies reacted with P. berghei sporozoite protein by enzyme-linked immunosorbent assay, Western blot and indirect immunofluorescence antibody, 2) six other monoclonal antibodies reacted with P. berghei sporozoites by ELISA and Western blot only. We studied whether these differences could be explained by reactivity in enzyme-linked immunosorbent assay with different P. berghei circumsporozoite peptides. Although all P. gallinaceum monoclonal antibodies reacted with the P. berghei repeats, the first group reacted with a conserved peptide sequence, N1, whereas the second group did not. These results suggest that circumsporozoite proteins from P. gallinaceum and P. berghei share common epitopes. the biological significance of our finding is not yet clear. Indeed, the cross-reactive monoclonal antibodies giving a positive indirect immunofluorescence antibody with the P. berghei sporozoites only caused a borderline effect on the living P. berghei parasites in vitro as measured by inhibition of sporozoite infectivity. 相似文献
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ANDREW R. WHITELEY ANURADHA BHAT EMILIA P. MARTINS RICHARD L. MAYDEN M. ARUNACHALAM SILVA UUSI‐HEIKKILÄ A. T. A. AHMED JIWAN SHRESTHA MATTHEW CLARK DEREK STEMPLE LOUIS BERNATCHEZ 《Molecular ecology》2011,20(20):4259-4276
Understanding a wider range of genotype–phenotype associations can be achieved through ecological and evolutionary studies of traditional laboratory models. Here, we conducted the first large‐scale geographic analysis of genetic variation within and among wild zebrafish (Danio rerio) populations occurring in Nepal, India, and Bangladesh, and we genetically compared wild populations to several commonly used lab strains. We examined genetic variation at 1832 polymorphic EST‐based single nucleotide polymorphisms (SNPs) and the cytb mitochondrial gene in 13 wild populations and three lab strains. Natural populations were subdivided into three major mitochondrial DNA clades with an average among‐clade sequence divergence of 5.8%. SNPs revealed five major evolutionarily and genetically distinct groups with an overall FST of 0.170 (95% CI 0.105–0.254). These genetic groups corresponded to discrete geographic regions and appear to reflect isolation in refugia during past climate cycles. We detected 71 significantly divergent outlier loci (3.4%) and nine loci (0.5%) with significantly low FST values. Valleys of reduced heterozygosity, consistent with selective sweeps, surrounded six of the 71 outliers (8.5%). The lab strains formed two additional groups that were genetically distinct from all wild populations. An additional subset of outlier loci was consistent with domestication selection within lab strains. Substantial genetic variation that exists in zebrafish as a whole is missing from lab strains that we analysed. A combination of laboratory and field studies that incorporates genetic variation from divergent wild populations along with the wealth of molecular information available for this model organism provides an opportunity to advance our understanding of genetic influences on phenotypic variation for a vertebrate species. 相似文献
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Semiempirical modeling of abiotic and biotic factors controlling ecosystem respiration across eddy covariance sites 总被引:1,自引:0,他引:1
MIRCO MIGLIAVACCA MARKUS REICHSTEIN ANDREW D. RICHARDSON ROBERTO COLOMBO MARK A. SUTTON GITTA LASSLOP ENRICO TOMELLERI GEORG WOHLFAHRT NUNO CARVALHAIS ALESSANDRO CESCATTI MIGUEL D. MAHECHA LEONARDO MONTAGNANI DARIO PAPALE SÖNKE ZAEHLE ALTAF ARAIN ALMUT ARNETH T. ANDREW BLACK ARNAUD CARRARA SABINA DORE DAMIANO GIANELLE CAROLE HELFTER DAVID HOLLINGER WERNER L. KUTSCH PETER M. LAFLEUR YANN NOUVELLON CORINNA REBMANN HUMBERTO R.
Da ROCHA MIRCO RODEGHIERO OLIVIER ROUPSARD MARIA‐TERESA SEBASTIÀ GUENTHER SEUFERT JEAN‐FRANCOISE SOUSSANA MICHIEL K.
Van Der MOLEN 《Global Change Biology》2011,17(1):390-409
In this study we examined ecosystem respiration (RECO) data from 104 sites belonging to FLUXNET, the global network of eddy covariance flux measurements. The goal was to identify the main factors involved in the variability of RECO: temporally and between sites as affected by climate, vegetation structure and plant functional type (PFT) (evergreen needleleaf, grasslands, etc.). We demonstrated that a model using only climate drivers as predictors of RECO failed to describe part of the temporal variability in the data and that the dependency on gross primary production (GPP) needed to be included as an additional driver of RECO. The maximum seasonal leaf area index (LAIMAX) had an additional effect that explained the spatial variability of reference respiration (the respiration at reference temperature Tref=15 °C, without stimulation introduced by photosynthetic activity and without water limitations), with a statistically significant linear relationship (r2=0.52, P<0.001, n=104) even within each PFT. Besides LAIMAX, we found that reference respiration may be explained partially by total soil carbon content (SoilC). For undisturbed temperate and boreal forests a negative control of total nitrogen deposition (Ndepo) on reference respiration was also identified. We developed a new semiempirical model incorporating abiotic factors (climate), recent productivity (daily GPP), general site productivity and canopy structure (LAIMAX) which performed well in predicting the spatio‐temporal variability of RECO, explaining >70% of the variance for most vegetation types. Exceptions include tropical and Mediterranean broadleaf forests and deciduous broadleaf forests. Part of the variability in respiration that could not be described by our model may be attributed to a series of factors, including phenology in deciduous broadleaf forests and management practices in grasslands and croplands. 相似文献
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Postfire energy exchange in arctic tundra: the importance and climatic implications of burn severity
Fires produce land cover changes that have consequences for surface energy balance and temperature. Three eddy covariance towers were setup along a burn severity gradient (i.e. Severely, Moderately, and Unburned tundra) to determine the effect of fire and burn severity on arctic tundra surface energy exchange and temperature for three growing seasons (2008–2010) following the 2007 Anaktuvuk River fire. The three sites were well matched before the fire, experienced similar weather, and had similar energy budget closure, indicating that the measured energy exchange differences between sites were largely attributable to burn severity. Increased burn severity resulted in decreased vegetation and moss cover, organic layer depth, and the rate of postfire vegetation recovery. Albedo and surface greenness steadily recovered with Moderately matching Unburned tundra by the third growing season. Decreased albedo increased net radiation and partly fueled increased latent and ground heat fluxes, soil temperatures, and thaw depth. Decreases in moss cover and the organic layer also influenced the ground thermal regime and increased latent heat fluxes. These changes either offset or decreased the surface warming effect from decreased albedo, resulting in a small surface warming in Severely and a small surface cooling in Moderately relative to Unburned tundra. These results indicate that fires have a significant impact on surface energy balance and highlight the importance of moss and permafrost thaw in regulating arctic surface energy exchange and temperature. 相似文献
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FERNANDO P. RODRIGUES FLVIA S. ROCHA JOS E. GARCIA JOS F. GARCIA MARIO DE VIVO SERGIO R. MATIOLI 《Molecular ecology resources》2006,6(3):686-688
Five microsatellite loci were isolated and characterized within the woolly mouse opossum (Micoureus paraguayanus), a Neotropical marsupial, using an enrichment cloning procedure. Between four and seven alleles were detected per locus, with expected heterozygosity ranging from 0.358 to 0.560. These microsatellites should provide useful markers in a variety of genetic analyses to examine parentage, inbreeding, population structure and population dynamics in fragmented forest habitats. 相似文献
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