Poliomyelitis vaccine; a report on field trials in Southern Alameda County in 1954

Southern Alameda County was the only area in California to take part in the 1954 nationwide field trials for testing poliomyelitis vaccine. Besides the contribution made in evaluating the vaccine, other benefits were:1. Participation contributed to the control of a serious communicable disease.2. Inoculations of vaccine during the field trials in all probability prevented some cases of paralytic poliomyelitis among children in southern Alameda County.3. The community became better informed about poliomyelitis and more interested in the local health department.4. Local physicians had an opportunity to contribute to important research and to give a valuable community service.5. The staff of the Alameda County Health Department became better informed and better prepared for any future responsibilities in poliomyelitis immunization programs.Other conclusions reached as a result of participation in the 1954 field trials are that the initial planning of the study was hasty and not sufficiently representative of medical and administrative thinking. If the trials had been planned for 1955 instead of 1954 there would have been sufficient time for research to be completed, professional information to be assured and for sound design of study.     

Development in the Floating World: Defenses of Eggs and Embryos Against Damage from UV Radiation

Eggs and embryos of many aquatic organisms develop in the watercolumn and can experience ultraviolet radiation with potentiallydeleterious effects. This is especially vexing for floatingembryos that develop in the surface or neuston layer. Radiationdamage can be a particular problem for these embryos since thecell division cycle during the cleavage period is quite shortand often these cycles do not have mitotic checkpoints to insurefaithful transmission of DNA to the daughter cells. This couldresult in cell division with unrepaired DNA in the blastomeres,which could impact embryogenesis and the transmission of thegenome through the germ line. Described strategies to restrictradiation damage include mechanisms to limit oxidative damageand the use of sunscreens such as the mycosporines to curb radiationto sensitive targets. We describe a particularly ingenious useof sunscreens in the tunicate embryo, the use of extra-embryoniccells to shield the embryo from potentially harmful UV-A andUV-B radiation. We also raise questions regarding the natureof UV damage to embryos (is it DNA or also protein) and thecharacteristics of DNA repair in such embryos. It is likelythat unique mechanisms are present in floating embryos thatdevelop in this air-water interface to assure that cell andgenomic integrity are maintained in this challenging environment.     

The distribution of Naididae (Oligochaeta) in the littoral zone of selected lakes in North Wales and Shropshire

SUMMARY. The distribution of twenty species of Naididae found in seventeen lakes in North Wales and Shropshire is described. Fewer species were observed in the mountain lakes in Snowdonia in comparison to the more productive lakes of Anglesey and Shropshire.
Substrate was an important factor influencing the distribution of naids within lakes, A small number of sexually mature individuals was found in more productive lakes in the summer and autumn months. Sexually mature individuals of Stylaria lacustris were cultured in the laboratory; the population doubled its numbers, by asexual reproduction, in 12 days at 15°C. In Llyn Coron a natural population of S. lacustris doubled its numbers in 40 days. The gut contents of S. lacustris consisted of periphyton.     

Importance of consumptive and non-consumptive prey mortality in a coupled predator–prey system

1. Laboratory experiments were completed to identify the mechanisms by which the predatory flatworm, Dugesia tigrina , imposes mortality on its Aedes aegypti and Daphnia magna prey. Feeding trials were completed in glass microcosms which contained one of three – nine densities of small or large individuals of each prey species.
2. Mortality by Dugesia on small and large Aedes followed a type II functional response, whereas the mortality of Daphnia resembled a type III functional response. Prey mortality imposed by Dugesia consisted of consumptive and non-consumptive elements. Non-consumptive mortality occurred when prey individuals trapped in mucus trails subsequently died but were not ingested.
3. Additional experiments were conducted to quantify consumptive (capture followed by ingestion) and non-consumptive mortality (death not followed by ingestion).
4. Consumptive mortality followed a type II functional response for small and large individuals of both prey species, whereas non-consumptive mortality increased with prey density, although the relationships differed with prey species and size. The non-consumptive mortality of large Daphnia increased at an accelerating rate with prey density and exceeded consumptive mortality at intermediate prey abundances. In contrast, non-consumptive mortality of small Aedes and small Daphnia was lower than consumptive mortality and either increased with prey density at a decelerating (small Aedes ) or accelerating (small Daphnia ) rate.
5. These results suggest that the importance of consumptive and non-consumptive mortality to total prey mortality needs to be considered when modelling predator–prey dynamics.     

Trophic level asynchrony in rates of phenological change for marine,freshwater and terrestrial environments

Recent changes in the seasonal timing (phenology) of familiar biological events have been one of the most conspicuous signs of climate change. However, the lack of a standardized approach to analysing change has hampered assessment of consistency in such changes among different taxa and trophic levels and across freshwater, terrestrial and marine environments. We present a standardized assessment of 25 532 rates of phenological change for 726 UK terrestrial, freshwater and marine taxa. The majority of spring and summer events have advanced, and more rapidly than previously documented. Such consistency is indicative of shared large scale drivers. Furthermore, average rates of change have accelerated in a way that is consistent with observed warming trends. Less coherent patterns in some groups of organisms point to the agency of more local scale processes and multiple drivers. For the first time we show a broad scale signal of differential phenological change among trophic levels; across environments advances in timing were slowest for secondary consumers, thus heightening the potential risk of temporal mismatch in key trophic interactions. If current patterns and rates of phenological change are indicative of future trends, future climate warming may exacerbate trophic mismatching, further disrupting the functioning, persistence and resilience of many ecosystems and having a major impact on ecosystem services.     

Phosphoenolpyruvate Carboxylase from the Crassulacean PlantBryophyllum fedtschenkoiHametet Perrier: ACTIVITY CHANGES AND KINETIC BEHAVIOUR IN CRUDE EXTRACTS

The total activity (capacity) of phosphoenolpyruvate carboxylasein crude extracts of leaves of Bryophyllum fedtschenkoi, measuredon a fresh weight basis, varies considerably with leaf positionand with photoperiodic treatment. No diurnal variation in capacitywas detected under long – day or short – day conditions.The enzyme in freshly prepared crude extracts is about 50 timesmore sensitive to inhibition by malate at pH 7 than that inaged extracts, or in the fully purified state. Desensitizationin extracts proceeds rapidly unless protective measures aretaken, and appears to be irreversible. A pH-dependence studyshows that the effect of desensitization on the kinetic parametersof malate inhibition is identical to that of increasing thepH by 2.0 units over a wide range, but the maximum velocityat pH 7.8 is virtually unaffected. The significance of the resultsfor both the experimental determination of cyclic changes inenzyme capacity, and the theories concerning the mechanism ofa circadian rhythm of phosphoenolpyruvate carboxylase activityin vivo are discussed.     

Ultrastructure of the Eyespot and its Possible Significance in Phototaxis of Tetracystis excentrica*†

SYNOPSIS. The eyespot of the zoospore of Tetracystis excentrica (a green alga) has been studied by light and electron microscopy. In Tetracystis the eyespot consists of about 110 osmiophilic granules which form a plate in the anterior third of the cell. The granules are about 80 Å in diameter and are found in the outermost portion of the chloroplast; they commonly show hexagonal close packing and a hexagonal shape. The granules are confined positionally by the chloroplast envelope and an inner thylakoid. The plasmalemma over the eyespot is thickened and is separated from the chloroplast envelope by a 50 mμ space. The eyespot of Tetracystis is compared with others reported in the literature and the possible functional significance of these studies is discussed. The possibility that the eyespot plate in Tetracystis serves as a shading device rather than the primary photoreceptor is considered.