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1.
Based on its proven anabolic effects on bone in osteoporosis patients, recombinant parathyroid hormone (PTH1-34) has been evaluated as a potential therapy for skeletal repair. In animals, the effect of PTH1-34 has been investigated in various skeletal repair models such as fractures, allografting, spinal arthrodesis and distraction
osteogenesis. These studies have demonstrated that intermittent PTH1-34 treatment enhances and accelerates the skeletal repair process via a number of mechanisms, which include effects on mesenchymal
stem cells, angiogenesis, chondrogenesis, bone formation and resorption. Furthermore, PTH1-34 has been shown to enhance bone repair in challenged animal models of aging, inflammatory arthritis and glucocorticoid-induced
bone loss. This pre-clinical success has led to off-label clinical use and a number of case reports documenting PTH1-34 treatment of delayed-unions and non-unions have been published. Although a recently completed phase 2 clinical trial of PTH1-34 treatment of patients with radius fracture has failed to achieve its primary outcome, largely because of effective healing
in the placebo group, several secondary outcomes are statistically significant, highlighting important issues concerning the
appropriate patient population for PTH1-34 therapy in skeletal repair. Here, we review our current knowledge of the effects of PTH1-34 therapy for bone healing, enumerate several critical unresolved issues (e.g., appropriate dosing regimen and indications)
and discuss the long-term potential of this drug as an adjuvant for endogenous tissue engineering. 相似文献
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Existence of Sites for Anions and Divalent Cations in the Solubilized γ-Aminobutyric Acid/Benzodiazepine Receptor Complex 总被引:2,自引:2,他引:0
This study evaluated the ability of gamma-aminobutyric acid (GABA), baclofen, monovalent anions, divalent cations, and various combinations thereof to protect solubilized benzodiazepine (BZ) receptors of types 1 and 2, when contained together on the complex, against heat inactivation. Neither anions, cations, nor GABA alone provided significant protection of solubilized BZ receptors against heat, but inclusion of monovalent anions or divalent cations together with 500 microM GABA did afford protection. Monovalent anions combined with GABA (500 microM) provided 50% to full protection. Divalent cations, such as CaCl2 (2.5 mM) or MgCl2 (2.5 mM) in the presence of GABA (500 microM) yielded 45% and 24% protection, respectively. Other divalent cations tested (Zn2+, Hg2+, Co2+, and Ni2+) were poor protectors, even when combined with GABA. Monovalent anions (200 mM NaCl) and divalent cations (5 mM CaCl2) when tested together provided no protection. Similarly, baclofen (the GABA-B agonist) provided no protection, either alone or together with anions or divalent cations. These results indicate that the independent but interacting recognition sites of GABA, BZ, anions, and divalent cations, previously detected in the membrane-bound state, are retained in the solubilized state. 相似文献
4.
The fungicide triarimol was tested for its effect on abscisic acid (ABA) accumulation in growing culturesof Cercospora rosicola. ABA accumulation was reduced by approximately 50% with 10–8 M triarimol. Growth ofC. rosicola, as measured by dry weight accumulation, was inhibited by triarimol concentrations at or greater than 10–7 M. These results are compared with those obtained with clomazone, ancymidol, and paclobutrazol, which inhibit ABA accumulation by 50% at concentrations of 5 × 10–5, 5 × 10–6, and 5 × 10–7 M, respectively. Triarimol, therefore, is among the most potent inhibitors of ABA biosynthesis reported to date. Feeding studies with [14C]mevalonic acid confirmed the inhibition of ABA biosynthesis by 5 × 10–8 M triarimol. These results support previous suggestions that one or more of the steps in the ABA biosynthetic pathway from mevalonic acid is catalyzed by cytochrome P-450. Feeding studies with 1-deoxy-[2H]-ABA in resuspended cultures ofC. rosicola show that the conversion of this substrate is not inhibited by triarimol. 相似文献
5.
This review of the Jesup North Pacific Expedition, still the most important expedition in American anthropology, gives an idea of the goals and hazards of fieldwork around 1900, the pitfalls of international research, the tensions between anthropologists and host populations, the careers of early anthropologists, the role of private philanthropy, and the character of anthropology at the turn of the century. Franz Boas was the Expedition's linchpin. His organization of the Expedition, the way he handled problems, and his personal concerns reveal aspects of his view of anthropology and some of his basic attitudes. 相似文献
6.
MORPHOLOGICAL CHANGES AND THE REQUIREMENTS FOR MACROMOLECULE SYNTHESIS DURING EXCYSTMENT OF ACANTHAMOEBA CASTELLANII
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Light and phase-contrast microscopic observations of excystment in Acanthamoeba castellanii have been used to classify cells in excysting populations as free trophozoites, or mature, activated, or preemergent cysts. These categories have been used to describe the kinetics of excystment. A pH of 7 and a temperature of 30°C have been found to be optimal for the activation of mature cysts. Both activation and emergence are inhibited by cycloheximide and actinomycin D, but neither process is much affected by hydroxyurea. Cell-free extracts of high molecular weight components of cyst cytoplasm can support protein synthesis in vitro, although less efficiently than similar extracts from trophozoites. Evidence indicates that some of the functional RNA in the cyst extracts is synthesized before excystment. 相似文献
7.
A case of emphysematous pyelonephritis with perirenal gas is presented. This patient underwent vigorous medical treatment followed by nephrectomy and survived. This condition has a high mortality and should be distinguished from less severe infections where gas is confined to the collecting system. This case and others previously reported suggest that treatment should initially be medical, followed by early surgical intervention consisting of either drainage or nephrectomy depending upon the degree of renal involvement. 相似文献
8.
Youssef Dalia El-Bakatoushi Ranya Elframawy Asmaa El-Sadek Laila Badan Ghada El 《Journal of plant research》2023,136(3):305-322
Journal of Plant Research - The current study examined the phylogenetic pattern of medicinal species of the family Apiaceae based on flavonoid groups production, as well as the overall mechanism of... 相似文献
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Summary Adverse effects resulting from fertilization with high rates of ammonium sulphate were determined on a kikuyu grass (Pennisetum clandestinum) pasture grown on a krasnozem in a sub-tropical environment. Corrective fertilizer practices using lime and phosphorus were evaluated.Ammonium sulphate application (336 kg N/ha/annum for 4 years followed by 672 kg N/ha/annum for 2 years) decreased soil pH from 5.0 to 4.0. Under these conditions, soluble Al in the soil increased, while exchangeable Ca, Mg, and K decreased. Concentrations of Ca, Mo, and P in the kikuyu tops were lowered, while concentrations of Mn were raised. Liming to pH 5.5 promoted growth more at 672 kg N/ha/annum than at 134 kg N/ha/annum, while generally little further yield response occurred as soil pH was raised to about 6.0. Liming increased the concentrations of P, Ca, N, and Mo but decreased Mn in kikuyu tops.Phosphorus application decreased soluble aluminium in the soil in all nitrogen treatments, but only increased kikuyu yield where 672 kg N/ha/annum was applied. It did not alter plant chemical composition, except for an increase in P concentration.Yield increases to liming and P were attributed to the alleviation of Al toxicity in the high N treatments. Lime responses in low N treatments were due to improved N nutrition resulting from mineralization of organic N.Lime application reduced the amount of N fertilizer required for maximum growth of kikuyu from 672 kg N/ha/annum on the unlimed soil to 134 kg N/ha/annum, while maintaining an adequate level of nutrients in the herbage and avoiding the problems of excess soil acidity. re]19760622 相似文献