排序方式: 共有125条查询结果,搜索用时 358 毫秒
1.
Aude Guénolé Rohith Srivas Kees Vreeken Ze Zhong Wang Shuyi Wang Nevan J. Krogan Trey Ideker Haico van Attikum 《Molecular cell》2013,49(2):346-358
- Download : Download high-res image (256KB)
- Download : Download full-size image
2.
3.
INO80 and gamma-H2AX interaction links ATP-dependent chromatin remodeling to DNA damage repair 总被引:31,自引:0,他引:31
Morrison AJ Highland J Krogan NJ Arbel-Eden A Greenblatt JF Haber JE Shen X 《Cell》2004,119(6):767-775
4.
5.
AUXIN RESPONSE FACTOR (ARF)-mediated signaling conveys positional information during embryonic and postembryonic organogenesis and mutations in MONOPTEROS (MP/ARF5) result in severe patterning defects during embryonic and postembryonic development. Here we show that MP patterning activity is largely dispensable when the presumptive carboxypeptidase ALTERED MERISTEM PROGRAM 1 (AMP1) is not functional, indicating that MP is primarily necessary to counteract AMP1 activity. Closer inspection of the single and double mutant phenotypes reveals antagonistic influences of both genes on meristematic activities throughout the Arabidopsis life cycle. In the absence of MP activity, cells in apical meristems and along the paths of procambium formation acquire differentiated identities and this is largely dependent on differentiation-promoting AMP1 activity. Positions of antagonistic interaction between MP and AMP1 coincide with MP expression domains within the larger AMP1 expression domain. These observations suggest a model in which auxin-derived positional information through MP carves out meristematic niches by locally overcoming a general differentiation-promoting activity involving AMP1. 相似文献
6.
7.
Exploration of the function and organization of the yeast early secretory pathway through an epistatic miniarray profile 总被引:23,自引:0,他引:23
Schuldiner M Collins SR Thompson NJ Denic V Bhamidipati A Punna T Ihmels J Andrews B Boone C Greenblatt JF Weissman JS Krogan NJ 《Cell》2005,123(3):507-519
We present a strategy for generating and analyzing comprehensive genetic-interaction maps, termed E-MAPs (epistatic miniarray profiles), comprising quantitative measures of aggravating or alleviating interactions between gene pairs. Crucial to the interpretation of E-MAPs is their high-density nature made possible by focusing on logically connected gene subsets and including essential genes. Described here is the analysis of an E-MAP of genes acting in the yeast early secretory pathway. Hierarchical clustering, together with novel analytical strategies and experimental verification, revealed or clarified the role of many proteins involved in extensively studied processes such as sphingolipid metabolism and retention of HDEL proteins. At a broader level, analysis of the E-MAP delineated pathway organization and components of physical complexes and illustrated the interconnection between the various secretory processes. Extension of this strategy to other logically connected gene subsets in yeast and higher eukaryotes should provide critical insights into the functional/organizational principles of biological systems. 相似文献
8.
9.
10.
Jeffrey Fillingham Michael-Christopher Keogh Nevan J Krogan 《Biochimie et biologie cellulaire》2006,84(4):568-577
One of the earliest responses to a DNA double-strand break (DSB) is the carboxy-terminal phosphorylation of budding yeast H2A (metazoan histone H2AX) to create gammaH2A (or gammaH2AX). This chromatin modification stretches more than tens of kilobases around the DSB and has been proposed to play numerous roles in break recognition and repair, although it may not be the primary signal for many of these events. Studies suggest that gammaH2A(X) has 2 more direct roles: (i) to recruit cohesin around the DSB, and (ii) to maintain a checkpoint arrest. Recent work has identified other factors, including chromatin remodelers and protein phosphatases, which target gammaH2A(X) and regulate DSB repair/recovery. 相似文献