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1.
Shaginian IA Khmel' IA Romanova IuM Veselova MA Chernukha MIu Chernin LS Sidorenko SV Lipasova VA Kovtun VP Alekseeva GV Alekseeva NV Stepanova TV Gintsburg AL 《Molekuliarnaia genetika, mikrobiologiia i virusologiia》2003,(4):15-20
Described in the paper are characteristics of B. cepacia clinical strains isolated from patients at Moscow hospitals. The strains were investigated for the presence of proteolytic, chitinolytic, hemolytic and lipase activities as well as for presence of components of the "Quorum sensing" gene activity regulatory system by using biological test-systems and in the polymerase chain reaction with primers to genes cepI and cepR. 相似文献
2.
V. A. Toptikov V. M. Totsky T. G. Alieksieieva O. O. Kovtun 《Cytology and Genetics》2017,51(4):253-262
Spectra of isozymes encoded by alleles of eight different genetic loci have been analyzed in order to characterize the genetic structure of different rapa whelk communities in the northwestern part of the Black Sea. The distribution of genotypes and the corresponding alleles deviated significantly from that predicted by the Hardy-Weinberg equation in the case of four of the eight loci investigated. Rapa whelk communities investigated were characterized by slight predominance of heterozygotes over homozygotes, this being indicative of the absence of close inbreeding among the members of specific communities. F- and G-statistics and the calculation of genetic distances according to the method developed by Nei showed that genetic differentiation between rapa whelk communities from different aquatories was insignificant. 相似文献
3.
Two compounds that deplete glutathione (buthionine sulfoximine and diethyl maleate) with different mechanisms of action decrease body temperature and increase tolerance to complete global cerebral ischemia, both correlating closely with the glutathione concentration decrease. Glutathione apparently participates in the regulations of these functional parameters. GSH diethyl ester does not influence the latter, though it increases moderately the GSH concentration. Injection of GSH ester into the cerebral ventricles or subcutaneously selectively increases the GSH level in the brain and liver. An influence of the brain on the glutathione system in the liver was revealed. Diethyl maleate and GSH ester increase the activity of glutathione metabolizing enzymes under certain conditions. 相似文献
4.
Viktoriya Sokolova Anna Kovtun Rolf Heumann Matthias Epple 《Journal of biological inorganic chemistry》2007,12(2):174-179
Calcium phosphate nanoparticles were prepared by precipitation from water and were then functionalized by DNA. These particles
are taken up by living cells and function as gene transfer agents, i.e., the DNA is brought into a cell’s nucleus and is incorporated
there into the cell’s genome (transfection). DNA which encodes for enhanced green fluorescent protein leads to green fluorescence
of successfully transfected cells. By adding the red-fluorescing marker tetramethylrhodamine isothiocyanate–bovine serum albumin
(TRITC-BSA) to the nanoparticles, their pathway into the cell and within the cell could be followed by fluorescence microscopy.
A clear correlation between the uptake of nanoparticles and the efficiency of transfection was found. Aggregates of DNA/TRITC-BSA
alone were not able to enter the cells, i.e., the inorganic nanoparticles are necessary as a carrier through the cell membrane. 相似文献
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Vasin MV Ushakov IB Kovtun VIu Komarova SN Semenova LA Galkin AA 《Radiatsionnaia biologiia, radioecologiia / Rossi?skaia akademiia nauk》2004,44(1):68-71
In experiments on mice radioprotective properties of indraline, phenyleprine and melatonin were compared at topical application as an ointment at a place of local exposure of animal hind to a dose of 38.3 Gy of 60Co gamma-quanta. Factor of dose reduction was 1.27-1.32 for indraline (1-10% ointment) and 1.29 for phenyleprine (0.25% ointment). Antioxidants were low efficient at radiation skin burn. At later local radiation injuries, such as hind contracture, the efficiency of indraline was 1.33-1.5, that of phenyleprine was 1.28, and that of melatonine (2 and 5% ointment) was 1.23-1.47. 相似文献
9.
Fumonisin B1-induced cell death in arabidopsis protoplasts requires jasmonate-, ethylene-, and salicylate-dependent signaling pathways 总被引:3,自引:0,他引:3 下载免费PDF全文
We have established an Arabidopsis protoplast model system to study plant cell death signaling. The fungal toxin fumonisin B1 (FB1) induces apoptosis-like programmed cell death (PCD) in wild-type protoplasts. FB1, however, only marginally affects the viability of protoplasts isolated from transgenic NahG plants, in which salicylic acid (SA) is metabolically degraded; from pad4-1 mutant plants, in which an SA amplification mechanism is thought to be impaired; or from jar1-1 or etr1-1 mutant plants, which are insensitive to jasmonate (JA) or ethylene (ET), respectively. FB1 susceptibility of wild-type protoplasts decreases in the dark, as does the cellular content of phenylalanine ammonia-lyase, a light-inducible enzyme involved in SA biosynthesis. Interestingly, however, FB1-induced PCD does not require the SA signal transmitter NPR1, given that npr1-1 protoplasts display wild-type FB1 susceptibility. Arabidopsis cpr1-1, cpr6-1, and acd2-2 protoplasts, in which the SA signaling pathway is constitutively activated, exhibit increased susceptibility to FB1. The cpr6-1 and acd2-2 mutants also constitutively express the JA and ET signaling pathways, but only the acd2-2 protoplasts undergo PCD in the absence of FB1. These results demonstrate that FB1 killing of Arabidopsis is light dependent and requires SA-, JA-, and ET-mediated signaling pathways as well as one or more unidentified factors activated by FB1 and the acd2-2 mutation. 相似文献