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Identification and expression of water stress- and abscisic acid-regulated genes in a drought-tolerant sunflower genotype 总被引:17,自引:0,他引:17
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Karine Cahier Damien Piel Rubén Barcia-Cruz David Goudenège K. Mathias Wegner Marc Monot Jesús L. Romalde Frédérique Le Roux 《Environmental microbiology》2023,25(8):1424-1438
Phages depend on their bacterial hosts to replicate. The habitat, density and genetic diversity of host populations are therefore key factors in phage ecology, but our ability to explore their biology depends on the isolation of a diverse and representative collection of phages from different sources. Here, we compared two populations of marine bacterial hosts and their phages collected during a time series sampling program in an oyster farm. The population of Vibrio crassostreae, a species associated specifically to oysters, was genetically structured into clades of near clonal strains, leading to the isolation of closely related phages forming large modules in phage–bacterial infection networks. For Vibrio chagasii, which blooms in the water column, a lower number of closely related hosts and a higher diversity of isolated phages resulted in small modules in the phage–bacterial infection network. Over time, phage load was correlated with V. chagasii abundance, indicating a role of host blooms in driving phage abundance. Genetic experiments further demonstrated that these phage blooms can generate epigenetic and genetic variability that can counteract host defence systems. These results highlight the importance of considering both the environmental dynamics and the genetic structure of the host when interpreting phage–bacteria networks. 相似文献
5.
Escherichia coli Heat Shock Protein DnaK: Production and Consequences in Terms of Monitoring Cooking
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Karine Seyer Martin Lessard Gabriel Piette Monique Lacroix Linda Saucier 《Applied microbiology》2003,69(6):3231-3237
Through use of commercially available DnaK proteins and anti-DnaK monoclonal antibodies, a competitive enzyme-linked immunosorbent assay was developed to quantify this heat shock protein in Escherichia coli ATCC 25922 subjected to various heating regimens. For a given process lethality (F7010 of 1, 3, and 5 min), the intracellular concentration of DnaK in E. coli varied with the heating temperature (50 or 55°C). In fact, the highest DnaK concentrations were found after treatments at the lower temperature (50°C) applied for a longer time. Residual DnaK after heating was found to be necessary for cell recovery, and additional DnaK was produced during the recovery process. Overall, higher intracellular concentrations of DnaK tended to enhance cell resistance to a subsequent lethal stress. Indeed, E. coli cells that had undergone a sublethal heat shock (105 min at 55°C, F7010 = 3 min) accompanied by a 12-h recovery (containing 76,786 ± 25,230 molecules/cell) resisted better than exponentially growing cells (38,500 ± 6,056 molecules/cell) when later heated to 60°C for 50 min (F7010 = 5 min). Results reported here suggest that using stress protein to determine cell adaptation and survival, rather than cell counts alone, may lead to more efficient heat treatment. 相似文献
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Estimating estuarine residence times in the Westerschelde (The Netherlands) using a box model with fixed dispersion coefficients 总被引:13,自引:6,他引:7
The residence time of the water masses in the Westerschelde estuary was determined using a simple compartment-model that simulates the advective-diffusive transport of a conservative dissolved substance (chlorinity). The residence time of a water parcel in the upstream part of the estuary (i.e. the time needed for this water parcel to leave the estuary) varied from about 50 days in winter to about 70 days in summer. The most seaward compartment had residence times of about 10-15 days.Dispersive coefficients that are fixed in time were able to reproduce the observed salinity distributions very well in the Westerschelde. They were obtained by calibration on observed chlorinities. It is argued that the apparent relationship of dispersive coefficients with freshwater flow, which is observed in certain studies, could (partly) reflect the deviation from steady state conditions which are required assumptions to calculate these dispersive coefficients directly from salinity profiles. 相似文献
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Meiofauna from the intertidal zone of five European estuaries (Ems, Westerschelde, Somme, Gironde, Tagus) was investigated. Samples represented a cross section of various benthic habitats from near-freshwater to marine, from pure silts to fine-sandy bottoms. The meiobenthic community comprised everywhere a fauna strongly dominated by nematodes, with meiobenthic density increasing with increasing salinity. The Ems differed from the other estuaries due to the presence of a well developed community of Copepods, Gastrotrichs, large Ciliates and/or soft-shelled Foraminiferans in some sites. The Westerschelde stood out due to the near-absence of harpacticoid copepods and, as in the Tagus, the lower meiobenthic densities in the marine part of the estuary. For nematode community analysis, we also included data from the Tamar which were obtained from the literature (Warwick &; Gee, 1984). This resulted in the enumeration of 220 species, belonging to 102 genera, each with a characteristic distribution along the salinity, sedimentary and latitudinal gradients. Using the multivariate technique CANOCO, a zonation along these different physicochemical determinants was observed as well although salinity and sediment characteristic (scale of hundreds of meters to kilometers) proved to be more important in explaining community structure than latitudinal differences (scale of hundreds of kilometers). Nematode diversity was nearly entirely determined on the genus level and was positively related to salinity. Deviations from this general trend in the Gironde and the Tamar were attributed to sedimentary characteristics or to low macrobenthic predation. The presence of a typical opportunistic colonizing nematode species Pareurodiplogaster pararmatus in the low-salinity region of the Gironde could indicate (organic?) pollution or disturbance of the intertidal mud-flats. 相似文献
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Carbon flows in the Westerschelde estuary (The Netherlands) evaluated by means of an ecosystem model (MOSES) 总被引:11,自引:11,他引:0
The autotrophic production and heterotrophic consumption of organic matter in the Westerschelde, a highly turbid and eutrophic estuary in the Southwest Netherlands is examined by means of a dynamic simulation model. The model describes the ecologically relevant processes in thirteen spatial compartments and adequately fits most observed data.Three autotrophic processes are included in the model. Net pelagic photosynthetic production is relatively low (average 41 gC m–2 yr–1) and three spatial compartments near the turbidity maximum zone are respiratory sinks of phytoplankton biomass. According to the model, net phytobenthic primary production is more important than pelagic primary production in the upstream half of the Westerschelde. On the scale of the entire estuary, benthic primary production amounts to about 60% of pelagic primary production. Water-column nitrification, which is very important in the nitrogen cycle, is most pronounced near the turbidity zone where it accounts for the major autotrophic fixation of carbon (up to 27 g C m–2 yr–1). Viewed on the scale of the total estuary, however, the process is not very important.Less than 20% of total organic carbon input to the estuary is primary produced, the remainder is imported from waste discharges and from the river.The degree of heterotrophy of the Westerschelde estuary proved to be one of the highest yet reported. On average 380 g carbon per square metre is net lost per year (range 200–1200 gC m–2 yr–1). The yearly community respiration (bacterial mineralization, respiration of higher trophic levels and sedimentation) is 4 to 35 times (estuarine mean of 6) higher than the net production. This degree of heterotrophy is highest near the turbidity maximum and generally decreases from the freshwater to the seaward boundary. About 75% of all carbon losses can be ascribed to pelagic heterotrophic processes; the sediment is only locally important.Mineralisation rates are highest in the turbidity region, but as only a fraction of total carbon resides here, less than 20% of all organic carbon is lost in this part of the estuary. This result is in contradiction with a previous budget of the estuary, based on data of the early seventies, where more than 80% of all carbon was estimated to be lost in the turbidity zone. Part of this discrepancy is probably caused by changes that have occurred in the estuary since that time.Due to the high heterotrophic activity, nearly all imported and in situ produced carbon is lost in the estuary itself and the Westerschelde is an insignificant source of organic matter to the coastal zone.The model estuary acts as a trap for reactive organic matter, both from the land, from the sea or in situ produced. Internal cycling, mainly in the water column, results in the removal of most of the carbon while the more refractory part is exported to the sea. 相似文献
9.
Arques Didier G.; Michel Christian J.; Orieux Karine 《Bioinformatics (Oxford, England)》1992,8(1):5-14
The software AGE (Analysis of Gene Evolution) has been developedboth to study a genetic reality, i. e. the identification ofstatistical properties in genes (e.g. periodicities), and tosimulate this observed genetic reality, by models of molecularevolution. AGE has two types of models: (i) models of sequencecreation from oligonucleotides: concatenation model in seriesof an oligonucleotide, independent (or Markov) mixing modelof oligonucleotides according to given probabilities (or a Markovmatrix); (ii) models of sequence evolution from created sequences:insertion/deletion process of (mono, di, tri)nucleot-ides, basemutation process. The study of a reality and the developmentof simulation models are based on several new algorithms: approximatedsimulation and exact calculus to compute various autocorrelationfunctions, Fourier transformation of autocorrelation curves,recognition of a curve form, etc. AGE is implemented on IBMor compatible microcomputers and can be used by biologists withoutany computer knowledge to identify statistical properties intheir newly determined DNA sequence and to explain them by modelsof molecular evolution. 相似文献
10.
Robo4 maintains vessel integrity and inhibits angiogenesis by interacting with UNC5B 总被引:1,自引:0,他引:1
Koch AW Mathivet T Larrivée B Tong RK Kowalski J Pibouin-Fragner L Bouvrée K Stawicki S Nicholes K Rathore N Scales SJ Luis E del Toro R Freitas C Bréant C Michaud A Corvol P Thomas JL Wu Y Peale F Watts RJ Tessier-Lavigne M Bagri A Eichmann A 《Developmental cell》2011,20(1):33-46
Robo4 is an endothelial cell-specific member of the Roundabout axon guidance receptor family. To identify Robo4 binding partners, we performed a protein-protein interaction screen with the Robo4 extracellular domain. We find that Robo4 specifically binds to UNC5B, a vascular Netrin receptor, revealing unexpected interactions between two endothelial guidance receptors. We show that Robo4 maintains vessel integrity by activating UNC5B, which inhibits signaling downstream of vascular endothelial growth factor (VEGF). Function-blocking monoclonal antibodies against Robo4 and UNC5B increase angiogenesis and disrupt vessel integrity. Soluble Robo4 protein inhibits VEGF-induced vessel permeability and rescues barrier defects in Robo4(-/-) mice, but not in mice treated with anti-UNC5B. Thus, Robo4-UNC5B signaling maintains vascular integrity by counteracting VEGF signaling in endothelial cells, identifying a novel function of guidance receptor interactions in the vasculature. 相似文献