Suki MY Lee  Renee WY Chan  Jennifer L Gardy  Cheuk-kin Lo  Alan DL Sihoe  Sara SR Kang  Timothy KW Cheung  Yi Guan  Michael CW Chan  Robert EW Hancock  Malik JS Peiris 《Respiratory research》2010,11(1):147

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Rahman Daiyan  Emma Catherine Lovell  Bosi Huang  Muhammad Zubair  Joshua Leverett  Qingran Zhang  Sean Lim  Jonathan Horlyck  Jianbo Tang  Xunyu Lu  Kourosh Kalantar‐Zadeh  Judy N. Hart  Nicholas M. Bedford  Rose Amal 《Liver Transplantation》2020,10(28)

In this study, scalable, flame spray synthesis is utilized to develop defective ZnO nanomaterials for the concurrent generation of H₂ and CO during electrochemical CO₂ reduction reactions (CO₂RR). The designed ZnO achieves an H₂/CO ratio of ≈1 with a large current density (j) of 40 mA cm^?2 during long‐term continuous reaction at a cell voltage of 2.6 V. Through in situ atomic pair distribution function analysis, the remarkable stability of these ZnO structures is explored, addressing the knowledge gap in understanding the dynamics of oxide catalysts during CO₂RR. Through optimization of synthesis conditions, ZnO facets are modulated which are shown to affect reaction selectivity, in agreement with theoretical calculations. These findings and insights on synthetic manipulation of active sites in defective metal‐oxides can be used as guidelines to develop active catalysts for syngas production for renewable power‐to‐X to generate a range of fuels and chemicals.  相似文献   

    

Hideaki Milton Arai  Alberto JS Duarte  Valéria Maria Natale 《Immunity & ageing : I & A》2006,3(1):9-7

Background  

a decline in immune and endocrine function occurs with aging. The main purpose of this study was to investigate the impact of long-term endurance training on the immune and endocrine system of elderly men. The possible interaction between these systems was also analysed.  相似文献   

    

JK MELICHAR  JS MYLES  CB EAP  DJ NUTT 《Addiction biology》2003,8(1):59-66

Substitute methadone prescribing is one of the main modes of treatment for opiate dependence. This study examined the relationship between methadone dose (measured by daily dose and methadone's active (R)‐enantiomer blood levels) and opiate receptor function. Nine subjects on substitute methadone (30‐90 mg daily) received three subcutaneous injections 1.5 hours apart (saline, 5 mg and 10 mg hydromorphone, a short‐acting opiate agonist) followed by measures of functional response in particular saccadic eye movements (SEMs), as well as self‐report measures. Ten mg of hydromorphone significantly slowed SEM parameters (peak velocity by 15%, p <0.005; peak acceleration by 20%, p <0.025; peak deceleration by 26%, p <0.025) and the SEM velocity changes correlated significantly with (R)‐methadone levels (r =0.844, p <0.005) and with the oral dose of methadone being taken (r =0.829, p <0.005). Although a similar trend was observed for 5 mg, this was not significant. These finding suggest that, at higher methadone doses (resulting in higher plasma concentrations), there is significant tolerance to the action of agonists. Such studies may help in refining our understanding of the actions of methadone and the SEM measure could help in defining the degree of tolerance in individuals using street heroin.  相似文献   

  总被引：8，自引：0，他引：8  

Delarbre  C; Kashi  Y; Boursot  P; Beckmann  JS; Kourilsky  P; Bonhomme  F; Gachelin  G 《Molecular biology and evolution》1988,5(2):120-133

We have examined the phylogenetic distribution of two t-specific markersamong representatives of various taxa belonging to the genus Mus. Thecentromeric TCP-1a marker (a testicular protein variant specific for allt-haplotypes so far studied) has also been apparently detected in severalnon-t representatives of the Mus IVA, Mus IVB, and probably M. cervicolorspecies. By contrast, a t-specific restriction- fragment-lengthpolymorphism allele (RFLP) of the telomeric alpha- globin pseudogene DNAmarker alpha-psi-4 was found only in animals belonging to the M.musculus-complex species either bearing genuine t- haplotypes or, like theM. m. bactrianus specimen studied here, likely to do so. This t-specificalpha-psi-4 RFLP allele was found to be as divergent from the RFLP allelesof the latter, non-t, taxonomical groups as it is from Mus 4A, Mus 4B, orM. spretus ones. These results suggest the presence of t-haplotypes and oft-specific markers in populations other than those belonging to the M. m.domesticus and M. m. musculus subspecies, implying a possible origin fort-haplotypes prior to the radiation of the most recent offshoot of the Musgenus (i.e., the spretus/domesticus divergence), some 1-3 Myr ago.  相似文献   

  总被引：3，自引：1，他引：3  

Rush  JS; van Leyen  K; Ouerfelli  O; Wolucka  B; Waechter  CJ 《Glycobiology》1998,8(12):1195-1205

The results described in the accompanying article support the model inwhich glucosylphosphoryldolichol (Glc-P-Dol) is synthesized on thecytoplasmic face of the ER, and functions as a glucosyl donor for threeGlc-P-Dol:Glc0-2Man9-GlcNAc2-P-P-Dol glucosyltransferases (GlcTases) in thelumenal compartment. In this study, the enzymatic synthesis and structuralcharacterization by NMR and electrospray-ionization tandem massspectrometry of a series of water-soluble beta-Glc-P-Dol analogs containing2-4 isoprene units with either the cis - or trans - stereoconfiguration inthe beta-position are described. The water- soluble analogs were (1) usedto examine the stereospecificity of the Glc-P-Dol:Glc0-2Man9GlcNAc2-P-P-Dolglucosyltransferases (GlcTases) and (2) tested as potential substrates fora membrane protein(s) mediating the transbilayer movement of Glc-P-Dol insealed ER vesicles from rat liver and pig brain. The Glc-P-Dol-mediatedGlcTases in pig brain microsomes utilized [3H]Glc-labeled Glc-P-Dol10,Glc-P-(omega, c )Dol15, Glc-P(omega, t,t )Dol20, and Glc-P-(omega, t,c)Dol20as glucosyl donors with [3H]Glc3Man9GlcNAc2-P-P-Dol the major productlabeled in vitro. A preference was exhibited for C15-20 substratescontaining an internal cis -isoprene unit in the beta-position. Inaddition, the water-soluble analog, Glc-P-Dol10, was shown to enter thelumenal compartment of sealed microsomal vesicles from rat liver and pigbrain via a protein-mediated transport system enriched in the ER. Theproperties of the ER transport system have been characterized. Glc-P-Dol10was not transported into or adsorbed by synthetic PC-liposomes orbovine erythrocytes. The results of these studies indicate that (1) theinternal cis -isoprene units are important for the utilization of Glc-P-Dolas a glucosyl donor and (2) the transport of the water- soluble analog mayprovide an experimental approach to assay the hypothetical \"flippase\"proposed to mediate the transbilayer movement of Glc-P-Dol from thecytoplasmic face of the ER to the lumenal monolayer.  相似文献   

  总被引：7，自引：22，他引：7  

JS Condeelis  DL Taylor 《The Journal of cell biology》1977,74(3):901-927

Motile extracts have been prepared from Dictyostelium discoideum by homogenization and differential centrifugation at 4 degrees C in a stabilization solution (60). These extracts gelled on warming to 25 degrees Celsius and contracted in response to micromolar Ca++ or a pH in excess of 7.0. Optimal gelation occurred in a solution containing 2.5 mM ethylene glycol-bis (β-aminoethyl ether)N,N,N',N'-tetraacetate (EGTA), 2.5 mM piperazine-N-N'-bis [2-ethane sulfonic acid] (PIPES), 1 mM MgC1(2), 1 mM ATP, and 20 mM KCI at ph 7.0 (relaxation solution), while micromolar levels of Ca++ inhibited gelation. Conditions that solated the gel elicited contraction of extracts containing myosin. This was true regardless of whether chemical (micromolar Ca++, pH >7.0, cytochalasin B, elevated concentrations of KCI, MgC1(2), and sucrose) or physical (pressure, mechanical stress, and cold) means were used to induce solation. Myosin was definitely required for contraction. During Ca++-or pH-elicited contraction: (a) actin, myosin, and a 95,000-dalton polypeptide were concentrated in the contracted extract; (b) the gelation activity was recovered in the material sqeezed out the contracting extract;(c) electron microscopy demonstrated that the number of free, recognizable F-actin filaments increased; (d) the actomyosin MgATPase activity was stimulated by 4- to 10-fold. In the absense of myosin the Dictyostelium extract did not contract, while gelation proceeded normally. During solation of the gel in the absense of myosin: (a) electron microscopy demonstrated that the number of free, recognizable F- actin filaments increased; (b) solation-dependent contraction of the extract and the Ca++-stimulated MgATPase activity were reconstituted by adding puried Dictyostelium myosin. Actin purified from the Dictyostelium extract did not gel (at 2 mg/ml), while low concentrations of actin (0.7-2 mg/ml) that contained several contaminating components underwent rapid Ca++ regulated gelation. These results indicated : (a) gelation in Dictyostelium extracts involves a specific Ca++-sensitive interaction between actin and several other components; (b) myosin is an absolute requirement for contraction of the extract; (c) actin-myosin interactions capable of producing force for movement are prevented in the gel, while solation of the gel by either physical or chemical means results in the release of F-actin capable of interaction with myosin and subsequent contraction. The effectiveness of physical agents in producting contraction suggests that the regulation of contraction by the gel is structural in nature.  相似文献   

    

Emelia Zukowski  Marco Sannella  Jack Donato Rockhold  Gabriella H. Kalantar  Jingting Yu  Sara SantaCruz-Calvo  Madison K. Kuhn  Nasun Hah  Ling Ouyang  Tzu-Wen Wang  Lyanne Murphy  Heather Marszalkowski  Kaleigh Gibney  Micah J. Drummond  Elizabeth A. Proctor  Hatice Hasturk  Barbara S. Nikolajczyk  Leena P. Bharath 《Aging cell》2023,22(11):e13996

Aging promotes numerous intracellular changes in T cells that impact their effector function. Our data show that aging promotes an increase in the localization of STAT3 to the mitochondria (mitoSTAT3), which promotes changes in mitochondrial dynamics and function and T-cell cytokine production. Mechanistically, mitoSTAT3 increased the activity of aging T-cell mitochondria by increasing complex II. Limiting mitoSTAT3 using a mitochondria-targeted STAT3 inhibitor, Mtcur-1 lowered complex II activity, prevented age-induced changes in mitochondrial dynamics and function, and reduced Th17 inflammation. Exogenous expression of a constitutively phosphorylated form of STAT3 in T cells from young adults mimicked changes in mitochondrial dynamics and function in T cells from older adults and partially recapitulated aging-related cytokine profiles. Our data show the mechanistic link among mitoSTAT3, mitochondrial dynamics, function, and T-cell cytokine production.  相似文献   

    

Ghasemi  Zahra  Kalantar  Kurosh  Amirghofran  Zahra 《Molecular biology reports》2021,48(2):1139-1150

FOXP3 X-linked gene has crucial roles in the development and function of regulatory T cells. We investigated the association of FOXP3 rs3761548, rs3761549 and rs2294021 single nucleotide polymorphisms (SNPs) with acute lymphoblastic leukemia (ALL) susceptibility and response to therapy. Genotyping was performed in 247 patients and 210 healthy subjects. We observed a higher frequency of rs3761548 A carriers and rs2294021 C carriers (p?<?0.04) in male patients, and lower frequencies of rs3761548 AC genotype (p?=?0.04) and rs2294021 CT genotype (p?=?0.01) in female patients compared to controls. ACC (p?=?0.04) and ATC haplotypes (p?=?0.002) were associated with susceptibility to ALL. There was a significant correlation between the genotypes of rs3761548 and rs2294021 SNPs with event-free survival (EFS) and overall survival (OS). The rs3761548 A genotype in male patients was associated with increased risk of relapse (p?<?0.0001), shorter EFS, increased death rate (p?=?0.002) and shorter OS compared to C genotype (p?=?0.001). Similar significant results were observed for the relation of rs2294021 C genotype with response to therapy in male patients. In females, patients with rs3761548 AC genotype had longer EFS (p?=?0.02) and those with rs2294021 CT had longer EFS and OS (p?<?0.005). According to haplotype analysis, patients carrying ACC or ATC haplotypes had the highest number of WBCs and shorter EFS or OS, and patients with CCT haplotype had the lowest number of WBCs and longer EFS or OS. These results provided evidence for the impact of these polymorphisms on susceptibility and response to therapy in children with ALL.

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Evidence for the biogenesis of more than 1,000 novel human microRNAs     

Marc R Friedl?nder  Esther Lizano  Anna JS Houben  Daniela Bezdan  Mónica Bá?ez-Coronel  Grzegorz Kudla  Elisabet Mateu-Huertas  Birgit Kagerbauer  Justo González  Kevin C Chen  Emily M LeProust  Eulàlia Martí  Xavier Estivill 《Genome biology》2014,15(4):R57

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