A STUDY ON VARIETAL DIFFERENCE IN FLOWERING HABITS OF SOYBEAN PLANTS AS FOLLOWED BY GRAFTING EXPERIMENTS

The material basis of varietal differences in flowering habitwas investigated from the standpoint that flowering is determinedby the balance in amounts of flowering promotor and inhibitor.The grafting method was used throughout the experiments. Late variety of Glycine max L. seems to produce flowering inhibitor(or inhibitors) under the conditions under which midseason varietyproduces flower. Early variety seems to produce flowering hormonewhich overcomes the flower-inhibiting action in the late variety. The amounts of flowering hormone produced under short day conditionbymidseason and late varieties were compared. The results showthat, under the short day condition, the midseason variety producesequal or smaller amounts of flowering hormone as compared withthe late variety. On the basis of these results, the mechanismby which the flowering habits are determined in various varietiesof soybean plants was discussed. (Received June 18, 1962; )     

Diurnal Rb+ Transport from Roots to the Laminar Pulvinus in Phaseolus vulgaris L.

The primary leaves of kidney bean (Phaseolus vulgaris L.) openunder light and close in the dark by the deformation of thepulvinus resulting from diurnal distribution changes of K⁺,Cl^–, organic acid (or H⁺) and NO₃^–. When Rb⁺ was added as a tracer of K⁺ to the seedlings throughtheir roots, it was transported to the pulvinus cells duringthe light period but not during the dark period. Transpirationoccurred vigorously in the light but almost stopped in the dark.We concluded that Rb⁺ absorbed by the roots was carried to thepulvinus by the transpiration stream. Phaseolus vulgaris L., pulvinus, Rb⁺, diurnal transport transpiration stream     

The role of calcium in turgor regulation in Chara longifolia

The salt-tolerant alga Chara longifolia (Robinson) is capable of regulating its turgor in response to hypotonic stress resulting from a decrease in the osmotic pressure of the medium. This regulatory process takes only 40 min in small cells (length ≤ 10 mm), but requires 3d in large cells (length ≥30mm). Turgor regulation in small cells is comprised of two phases, a fast phase reducing the increased turgor by about 25% in the First 5 min, and a second phase reducing the turgor to near the original value within 40 min. The second phase is inhibited by reducing the concentration of Ca²⁺ in the external medium from 4.6 to 0.01 mol m^?3; the first phase is less affected by the reduction of Ca²⁺. In the first 5 min of stress, the membrane depolarizes in a voltage-dependent fashion, electrical conductance of the membrane increases transiently and cytoplasmic streaming is inhibited. When the external Ca²⁺ concentration is lowered, conductance does not increase and streaming continues unaffected. In a low ionic strength medium, Ca²⁺ is not required in the medium for turgor regulation. To test the hypothesis that there is increased Ca²⁺ entry from the medium during turgor regulation, we measured the influx of ⁴⁵Ca²⁺ into the cell. We found an increased influx of Ca²⁺, from 18 to 36 nmol m^?2 s^?1 during the first 30 to 90 s following osmotic stress. This increase was evident only in cells below about 7 mm in length, and was more marked in smaller cells.     

Survival and death of Chara internodal cells in electrolyte solutions and calcium release from the cell wall

Abstract. Survival and death of Chara internodal cells were investigated in one of the alkali metal salts KCl, some of the alkali earth metal salts CaCl₂, Ca(NO₃)₂, MgCl₂, Mg(NO₃)₂, SrCl₂, Sr(NO₃)₂, BaCl₂ and Ba(NO₃)₂, potassium phosphate pH buffer solution (pH 7.0), Tris-maleate pH buffer solution (pH 7.0), HEPES (N-2-hydroxyethylpiperazine-N′-2-ethanesulphonic acid)-KOH (pH 7.0) pH buffer solution, calcium buffer solutions, and deionized water. Most of the internodal cells died within a day or a few days in KCl, MgCl₂, Mg(NO₃)₂, BaCl₂ and Ba(NO₃)₂ solutions of higher concentrations, calcium buffer solutions of pCa 6.0, 10.0 mol m^-3 potassium phosphate pH buffer solution and 10.0 mol m^-3 Trismaleate pH buffer solution. However, all of the internodal cells survived more than 10 d in deionized water, 80.0 mol m^-3 CaCl₂, 80.0 mol m^-3 Ca(NO₃)₂, 80.0 mol m^-3 SrCl₂, 80.0 mol m^-3 Sr(NO₃)₂ calcium buffer solutions of pCa 4.0 and pCa 5.0, and 10.0 mol m^-3 HEPES-KOH (pH 7.0) pH buffer solution. Addition of Ca²⁺ or Sr²⁺ to K⁺, Mg²⁺ and Ba²⁺ salt solutions increased the survival rates of the internodal cells. Calcium release from the internodal cell wall was measured in deionized water, KCl, NaCl, MgCl₂, CaCl₂, SrCl₂ and BaCl₂ solutions. Except in deionized water and CaCl₂ solution, most of the calcium binding to the cell wall was released within one or a few hours in respective electrolyte solutions. Thus, survival and death of the internodal cells in the electrolyte solutions tested were interpreted in terms of the calcium release from the cell wall and the cell membrane, and intrinsic ability of Sr²⁺ to maintain the cell membrane normal.