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目的:用2株新分离鉴定的细菌克隆与最近源种(Bacillus halodurans C-125)木聚糖酶基因的同源序列,并对其进行序列分析。方法:根据已发表的近源种(C-125)的保守区序列设计引物,扩增出与其同源的木聚糖酶基因,进而用pQE31载体对该基因进行表达。同时对表达蛋白的三维空间结构进行网络模拟分析,对蛋白同源性进行比对分析。结果:克隆的木聚糖酶基因片段分别为1284bp和1236bp,而且该基因也成功表达;该蛋白是(α/α)6折叠构象,与C-125三者同属于一个分支上。结论:通过分析、表达蛋白编码外切-1,4-β-木聚糖酶,属于M家族。这该新分离菌株木聚糖酶的研究和应用提供了重要线索。 相似文献
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Murat alayan Cigdem Sonmez Mehmet Senes Ataman Gonel Ozlem Gulbahar Nurbanu Bursa Derun Taner Osman Celik Fidanci Ali Aykut Ulgu Mustafa Mahir Abdulvahit Sozuer Naim Ata Suayip Birinci 《Journal of Medical Biochemistry》2022,41(4):450
BackgroundAcross the world, 25-hydroxyvitamin D (25-OHD) deficiency is a major health problem associated with many chronic diseases in the geriatric population. Prior to this study, there were no data regarding 25-OHD levels among individuals over the age of 65 in Turkey. The aim of this study was to assess 25-OHD levels and seasonal variations in these values among people over the age of 65 in Turkey.MethodsThis study included vitamin D measurements taken in 2016, 2017, and 2018 from the Turkish population over the age of 65. The age, gender, and seasonal average data of the study population were defined. The study data were obtained from the database of the Ministry of Health, and a Kolmogorov-Smirnov test was used to assess the distribution of the data. Medians and interquartile ranges (IQRs) were calculated for all categories, as the data were nonparametric.ResultsThe number of vitamin D measurements taken from the geriatric individuals included in this study was 305,329 for 2016, 576,452 for 2017, and 752,837 for 2018. The medians and IQRs of the 25-OHD levels in this population were 16 μg/L (IQR 7.45-24.55 μg/L) for 2016, 16.1 μg/L (IQR 7.8-24.4 μg/L) for 2017, and 16.4 μg/L (IQR 8.95-23.85 μg/L) for 2018.ConclusionsWhile the 25-OHD levels of older men tended to increase during the period of seasonal sunlight in Turkey, this variability was observed in elderly women. This suggests that older women tend to live more sedentary lives and have insufficient sun exposure. Overall, the median 25-OHD levels of individuals over the age of 65 tended to decrease each year. 相似文献
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Gulbahar Boyuk A. Arzu Yigit Ilkay Aydogan 《In vitro cellular & developmental biology. Animal》2018,54(9):640-647
Islet cell transplantation is a major treatment strategy for type I diabetes, and has proven to be effective for maintaining glucose homeostasis. However, this treatment requires an extended period of immunosuppression to prevent rejection and recurrent transplantation to maintain function. Thus, to enhance the properties of transplanted islet cells, we examined the effect of the co-culture of luteal cells, which secrete progesterone, on islet cell viability, functionality, and revascularization. It was found that islet viability and functionality were higher in the co-cultured group than in single cultures of islets at 48 and 96 h, in parallel with increased progesterone and vascular endothelial growth factor (VEGF) secretion from luteal cells. In the co-culture groups, VEGF levels at 48 and 96 h and CD31 levels at 48 h were significantly higher than those in the islet groups (p?<?0.001 and p?<?0.05, respectively), and basic fibroblast growth factor (bFGF) levels were increased at 96 h (p?<?0.001). Thus, co-culture with luteal cells may increase islet vascularity by enhancing VEGF and bFGF levels for up to 96 h, which could help to markedly increase the pre-transplantation time to allow for effective immunosuppression therapy. This method may also promote islet cell viability and functionality. Progesterone and angiogenic factors secreted from luteal cells may be responsible for these positive effects. 相似文献
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Sleep and Biological Rhythms - 相似文献
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Jan Willem Erisman Nelleke Domburg Wim de Vries Hans Kros Bronno de Haan Kaj Sanders 《中国科学:生命科学英文版》2005,48(Z2)
The Netherlands is "well known" for its nitrogen problems; it has one of the highest reactive nitrogen (Nr) emission densities in the world. It is a small country at the delta of several large European rivers. Ever since the industrial revolution, there has been a growing excess of nutrients and related emissions into the atmosphere (ammonia, nitrogen oxides and nitrous oxide) and into groundwater and surface water (nitrate), leading to a large range of cascading environmental impacts. Vehicular traffic, sewage and animal husbandry are the main sources of oxidized and reduced forms of Nr. This paper provides an overview of the origin and fate of nitrogen in the Netherlands, the various reported impacts of nitrogen, the Dutch and European policies to reduce nitrogen emissions and related impacts. In addition, ways are presented to go forward to potentially solve the problems in a European perspective. Solutions include the improvement of nitrogen efficiencies in different systems, technological options and education. 相似文献
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The intermediate filament (IF) synemin gene encodes three IF proteins (H 180, M 150, L 41 kDa) with overlapping distributions.
Synemin M was present early with vimentin and nestin. Synemin H was found later in the nervous system and mesodermic derivatives
concomitantly with angiogenesis and the migration of neural crest cells. Synemin L appeared later in neurons. A series of
in vitro cell cultures were done to identify the linkage between synemin isoforms and specific cell types of the central nervous
system (CNS). The neurons and glia from the brains of humans and rats were cultured and double immunostaining done with antibodies
against the H/M or L synemin isoforms and neural cell types (βIII-tubulin or NeuN) or astrocyte intermediate filaments (GFAP
or vimentin). In neurons of the CNS, synemin H/M were co-expressed with GFAP, vimentin or nestin in glial cells, whereas synemin
L was found in neurons. 相似文献
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Mitochondrial DNA (mtDNA) restriction-site maps for six species (10
strains) of the Drosophila montium subgroup were established. A total of 50
restriction sites were mapped, corresponding to 1.67% of the mtDNA genome.
On the basis of differences in the restriction sites, nucleotide divergence
(delta) was calculated for each pair of species (strains), and phylogenetic
trees were constructed by using distance- matrix and parsimony methods.
Comparison of the resultant phylogenetic trees shows that the sibling
species D. auraria and D. quadraria are closely related. At the other
extreme, considerable divergence was observed between the two strains of D.
serrata and between D. serrata and D. birchii, a finding that contrasts
with their grouping within the same species complex. Nevertheless, our data
indicate that these six oriental montium species are rather closely
related.
相似文献
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Baba Burcu Caliskan Mursel Boyuk Gulbahar Hacisevki Aysun 《Biochemistry. Biokhimii?a》2021,86(4):480-488
Biochemistry (Moscow) - Endoplasmic reticulum (ER) is very sensitive to the nutritional and energy states of the cells. Disruption of ER homeostasis leads to the accumulation of unfolded/misfolded... 相似文献
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