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Heterogeneous inbred family (HIF) analysis: a method for developing near-isogenic lines that differ at quantitative trait loci 总被引:10,自引:0,他引:10
M. R. Tuinstra G. Ejeta P. B. Goldsbrough 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(5-6):1005-1011
Abtract Analysis of near-isogenic lines (NILs) that differ at quantitative trait loci (QTL) can be an effective approach for the
detailed mapping and characterization of individual loci. Although NILs are useful for genetic and physiological studies,
the time and effort required to develop these lines have limited their use. Here we describe a procedure to identify NILs
for any region of the genome that can be analyzed with molecular or other genetic markers. The procedure utilizes molecular
markers to identify heterogeneous inbred families (HIFs) that segregate for a genomic region of interest. Each HIF is isogenic
at the majority of loci in the genome, but NILs differing for markers linked to QTL of interest can be extracted from segregating
families. The application of this procedure is described for two QTL associated with seed weight in sorghum. A population
of 98 HIFs was screened with two RAPD markers from different linkage groups that were associated with seed weight. Three segregating
families were identified for each marker. The progeny of these HIFs were characterized for the segregation of seed weight
and other yield components and for markers flanking each QTL. NILs derived from each HIF had significantly different seed
weights confirming the presence of at least two loci that influence seed weight in sorghum.
Received: 16 September 1996 / Accepted: 25 April 1997 相似文献
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Expression and stability of amplified genes encoding 5-enolpyruvylshikimate-3-phosphate synthase in glyphosate-tolerant tobacco cells 总被引:10,自引:0,他引:10
Yunxia Wang James D. Jones Stephen C. Weller Peter B. Goldsbrough 《Plant molecular biology》1991,17(6):1127-1138
Two distinct cDNAs for 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) were obtained from a glyphosate-tolerant tobacco cell line. The cDNAs were 89% identical and the predicted sequences of the mature proteins were greater than 83% identical with EPSPS proteins from other plants. Tobacco EPSPS proteins were more similar to those from tomato and petunia than Arabidopsis. One cDNA clone, EPSPS-1, represented a gene that was amplified in glyphosate-tolerant cells, while the gene for EPSPS-2 was unaltered in these cells. Consequently, EPSPS-1 mRNA was more abundant in tolerant than unselected cells, whereas EPSPS-2 mRNA was at relatively constant levels in these cell lines. Exposure of unselected cells and tobacco leaves to glyphosate produced a transient increase in EPSPS mRNA. However, glyphosate-tolerant cells containing amplified copies of EPSPS genes did not show a similar response following exposure to glyphosate. A significant proportion of the EPSPS gene amplification was maintained when tolerant cells were grown in the absence of glyphosate for eight months. Plants regenerated from these cells also contained amplified EPSPS genes. 相似文献
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The structure and transcription start site of a major potato tuber protein gene 总被引:12,自引:4,他引:12 下载免费PDF全文
M Bevan R Barker A Goldsbrough M Jarvis T Kavanagh G Iturriaga 《Nucleic acids research》1986,14(11):4625-4638
We have isolated recombinant lambda clones containing intact major tuber protein (patatin) genes and flanking sequences from the commercial tetraploid variety Maris Piper. The gene is composed of seven exons and six introns, spread over 4 kb of DNA. Nuclease mapping defined the 5' end of the mRNA approximately 45 bp upstream of the initiation codon. The 5' end of the gene is preceeded by a canonical TATA box sequence. The three known patatin genes encode proteins of nearly identical Mr but very different isoelectric points. The sequence of the gene does not indicate a role for patatin as one of the globulin class of plant storage proteins. 相似文献
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Conformational differences between two wheat (Triticum aestivum) ''high-molecular-weight'' glutenin subunits are due to a short region containing six amino acid differences. 总被引:1,自引:0,他引:1 下载免费PDF全文
'High-molecular-weight' (HMW, high-Mr) glutenin subunits are protein constituents of wheat (Triticum aestivum) seeds and are responsible in part for the viscoelasticity of the dough used to make bread. Two subunits, numbered 10 and 12, are the products of allelic genes. Their amino acid sequences have been derived from the nucleic acid sequences of the respective genes. Subunit 10 has fewer amino acids than subunit 12, but migrates more slowly on SDS/PAGE (polyacrylamide-gel electrophoresis). This anomaly is due to between one and six of the amino acid differences between the subunits, localized towards the C-terminal end of the proteins. This has been established by making chimaeric genes between the genes for subunits 10 and 12, transcribing and translating them in vitro and analysing the products by SDS/PAGE. The postulated conformational differences between subunits 10 and 12 are discussed in relation to current hypotheses for the structure of HMW glutenin subunits. 相似文献
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The complete sequence of the flax 5S DNA repeat is presented. Length heterogeneity is the consequence of the presence or absence of a single direct repeat and the majority of single base changes are transition mutations. No sequence variation has been found in the coding sequence. The extent of methylation of cytosines has been measured at one location in the gene and one in the spacer. The relationship between the observed sequence heterogeneity and the level of methylation is discussed in the context of the operation of a correction mechanism. 相似文献