全文获取类型
收费全文 | 876篇 |
免费 | 84篇 |
专业分类
960篇 |
出版年
2022年 | 5篇 |
2021年 | 12篇 |
2019年 | 7篇 |
2018年 | 12篇 |
2017年 | 14篇 |
2016年 | 12篇 |
2015年 | 34篇 |
2014年 | 31篇 |
2013年 | 41篇 |
2012年 | 46篇 |
2011年 | 51篇 |
2010年 | 41篇 |
2009年 | 41篇 |
2008年 | 43篇 |
2007年 | 47篇 |
2006年 | 29篇 |
2005年 | 25篇 |
2004年 | 38篇 |
2003年 | 30篇 |
2002年 | 35篇 |
2001年 | 30篇 |
2000年 | 30篇 |
1999年 | 33篇 |
1998年 | 13篇 |
1996年 | 9篇 |
1995年 | 13篇 |
1994年 | 11篇 |
1993年 | 9篇 |
1992年 | 9篇 |
1991年 | 7篇 |
1990年 | 8篇 |
1989年 | 7篇 |
1988年 | 7篇 |
1987年 | 7篇 |
1986年 | 12篇 |
1984年 | 8篇 |
1983年 | 5篇 |
1982年 | 8篇 |
1980年 | 5篇 |
1978年 | 11篇 |
1977年 | 6篇 |
1975年 | 6篇 |
1974年 | 6篇 |
1973年 | 8篇 |
1972年 | 11篇 |
1971年 | 4篇 |
1970年 | 8篇 |
1969年 | 10篇 |
1968年 | 8篇 |
1966年 | 6篇 |
排序方式: 共有960条查询结果,搜索用时 0 毫秒
1.
To determine the distribution of glucose carbons to the major metabolic pathways as a function of glucose and insulin concentrations in fed rat muscle, rat hindlimbs were perfused with and without 100 nM insulin in the presence of 1-20 mM [U-14C]-D-glucose. The incorporation of radioactivity into CO2, anionic metabolites released into the medium and muscle cell fractions were determined. The results indicate that the major metabolic fate of glucose taken up by muscle in the fed rat is conversion to anions released into the medium and that this process is saturable with increasing glucose concentrations. The data suggest that saturation of the glycolytic pathway is the major cause of the previously observed saturation of the glucose disposal rate associated with increasing glucose concentration. 相似文献
2.
John F. Foley Byron Th. Aftonomos 《In vitro cellular & developmental biology. Plant》1988,24(9):900-904
Summary Colonies of HeLa cells cultured in media supplemented with human or bovine serum or both can be morphologically described
as three types: diffuse, intermediate, and compact, with their modal distribution depending on the serum or sera added to
the growth medium. We have observed that for a particular medium or serum system, the percentage of compact colonies remains
fairly constant under normal culture conditions, 0.2%, whereas the diffuse and intermediate colonies vary over a much wider
range. The presence of certain substances as trypsin, heparin and Darvan in the medium favor the increase of compact colonies
at the expense of other types. Furthermore, we have discovered that colonial morphology is influenced by cocultivation of
the HeLa cells with human fibroblastlike cells, the compact colonies increasing as the density of the fibroblast element introduced
into the mixed cultures is increased. Subsequent investigation revealed that conditioned medium from confluent fibroblast
and HeLa cell cultures contained a factor(s), that significantly increased the percentage of compact colonies. The factor
is nondialyzable, heat-stable and can be neutralized by serum. Recorded in this presentation are preliminary observations
on the kinetics of colony formation and the interaction among the three HeLa cell colony types, the diffuse, the intermediate,
and the compact. The factor's effect on HeLa cell colonial morphology is time dependent and rapidly reversed if the factor(s)
is removed and fresh medium added. 相似文献
3.
4.
D H Foley 《Canadian journal of physiology and pharmacology》1986,64(1):55-61
The relationship between length and the responses of helical strips of rabbit femoral and coronary arteries to vasoactive agents was investigated by conducting concentration--response determinations at the length for maximum active force (Lmax) and a shorter length. The mean effective dose (ED50) values for norepinephrine (NE) were smaller when femoral artery strips were set at Lmax, in comparison to the values at the shorter length. Maximal relaxation of femoral artery strips by adenosine was greater when the strips were set at Lmax. However, adenosine ED50 values were smaller at Lmax only in groups of strips in which responses at Lmax were obtained prior to those at the shorter length. Experiments with coronary artery strips did not demonstrate consistent relationships between strip length and ED50 values for acetylcholine (ACh) or adenosine. The results of experiments with artery strips from normotensive and those from one-kidney, one-clip hypertensive rabbits were similar. Thus, the femoral artery data indicate that helical artery strip preparations may exhibit length-dependent sensitivity to a vasodilator agent as well as vasoconstrictor agents under certain experimental conditions. However, the coronary artery data suggest that length may not affect sensitivity of isolated artery preparations from all vascular beds in the same manner. 相似文献
5.
Biochemical characterization of complex formation by human erythrocyte spectrin, protein 4.1, and actin 总被引:9,自引:0,他引:9
Ternary complex formation between the major human erythrocyte membrane skeletal proteins spectrin, protein 4.1, and actin was quantified by measuring cosedimentation of spectrin and band 4.1 with F-actin. Complex formation was dependent upon the concentration of spectrin and band 4.1, each of which promoted the binding of the other to F-actin. Simultaneous measurement of the concentrations of spectrin and band 4.1 in the sedimentable complex showed that a single molecule of band 4.1 was sufficient to promote the binding of a spectrin dimer to F-actin. However, the molar ratio of band 4.1/spectrin in the complex was not fixed, ranging from approximately 0.6 to 2.2 as the relative concentration of added spectrin to band 4.1 was decreased. A mole ratio of 0.6 band 4.1/spectrin suggests that a single molecule of band 4.1 can promote the binding of more than one spectrin dimer to an actin filament. Saturation binding studies showed that in the presence of band 4.1 every actin monomer in a filament could bind at least one molecule of spectrin, yielding ternary complexes with spectrin/actin mole ratios as high as 1.4. Electron microscopy of such complexes showed them to consist of actin filaments heavily decorated with spectrin dimers. Ternary complex formation was not affected by alteration in Mg2+ or Ca2+ concentration but was markedly inhibited by KCl above 100 mM and nearly abolished by 10 mM 2,3-diphosphoglycerate or 10 mM adenosine 5'-triphosphate. Our data are used to refine the molecular model of the red cell membrane skeleton. 相似文献
6.
Theodore M. Liszczak Ph.D. Lorraine Foley Peter McL. Black 《Cell and tissue research》1986,246(2):379-385
Summary The experiments described herein use an in vitro preparation of choroid plexus to demonstrate that it is a vasopressin-responsive organ by morphologic criteria. Choroid plexus from rats was incubated for one hour in graded concentrations of arginine vasopressin (AVP). Within physiologic range of molar concentration, incubation in vasopressin induced a decrease in basal and lateral spaces in choroid plexus epithelial cells as well as an increase in number of dark cells. The number of cells with basal spaces decreased significantly from 82.7±9.2 in control tissue to 19±18 in tissue incubated in 10-12 M AVP; similarly, the number with lateral cellular spaces decreased from 20±8.8 to 7.6±2.2 cells in 10-10 M AVP. Dark cells increased in number from 3.8±2.6 in control conditions to 49±4 with 10-9 M vasopressin. These data suggest important effects of arginine vasopressin in cerebrospinal fluid (CSF) on choroid plexus, compatible with enhanced fluid transport across choroid epithelial cells. 相似文献
7.
8.
9.
Borrelia burgdorferi is a spirochete pathogen transmitted among warm-
blooded hosts by ixodid ticks. Frequency-dependent selection for variant
outer-surface proteins might be expected to arise in this species, since
rare variants are more likely to avoid immune surveillance in previously
infected hosts. We sequenced the OspA and OspB genes of nine North American
strains and compared them with nine strains previously described. For each
gene, the mean number of synonymous substitutions per synonymous site and
the mean number of nonsynonymous substitutions per nonsynonymous site show
only a twofold excess of silent mutations. Synonymous rates vary widely
along the OspB protein. Some regions show a significant excess of silent
substitutions, while divergence in other regions is constrained by biased
base composition or selection. The presence, in antigenically important
regions of the protein, of significant variation among strains, as well as
evidence for recombination among strains, should be considered in attempts
to develop vaccines against this disease.
相似文献
10.