Spliceosome-targeted therapies trigger an antiviral immune response in triple-negative breast cancer

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The antigenic anatomy of SARS-CoV-2 receptor binding domain

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Negative regulation of AMPK signaling by high glucose via E3 ubiquitin ligase MG53

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SENP3 senses oxidative stress to facilitate STING-dependent dendritic cell antitumor function

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Analysis of single nucleotide polymorphisms in the promoter region of interleukin-10 by denaturing high-performance liquid chromatography.

Interleukin-10 (IL10), an anti-inflammatory cytokine, has been implicated in a variety of immune- and inflammatory-related diseases. We investigated the following SNPs: -1082, -819, -592 in the promoter region of IL10 in a normal (control) population and selected diseases: breast cancer (BrCa), systemic lupus erythematosus (SLE), and B-cell chronic lymphocytic leukemia (B-CLL) by denaturing high-performance liquid chromatography (DHPLC) and found distinct genotype and haplotype patterns. DHPLC was performed using the Transgenomic WAVE instrument, a mutational discovery tool that allows for high throughout analysis of SNPs. The principle of DHPLC is based on separation of homo- and heteroduplex formation of individual polymerase chain reaction products at specific melting temperatures and set gradients. The melting temperature selected for each SNP was based on size and sequence of the polymerase chain reaction product (for -1082, 57 degrees C; for -819, 58 degrees C; and for -592, 59.2 degrees C). Before fragment mutational analysis, all samples were denatured at 95 degrees C and slowly reannealed to allow for reassociation of different strands. Heteroduplex samples were easily distinguished from homoduplex samples. In order to identify wild type from homozygous mutant, two homoduplex polymerase chain reaction samples had to be mixed together, denatured at 95 degrees C and reannealed. The homozygous mutant, when combined with wild type, displayed a double peak on chromatogram. Once distinct chromatograms were established for each of the SNPs and the nucleotide changes confirmed by sequencing, genotype and haplotype frequencies were tabulated for the groups studied.     

苹果Co基因的SSR标记定位（简报）

Columnar apple is an important genetic resource for tree form breeding of apple. In this study, 106 individuals of the F1 population derived from 'Spur Fuji' (coco)x 'Telamon'(Coco) were used as plant materials for screening SSR markers linked to gene. By bulked segregating analysis (BSA), eight SSR markers from the tenth linkage group of apple genome were tested. Finally, three of them, COL, CH02a10 and CH03d11, were identified to be SSR markers of Co gene. Linkage analysis showed that the genetic distance of COL, CH02a10 and CH03d11 to Co locus was 15.3cM, 22.2cM and 3.9 cM, respectively. On the linkage map of these markers, Co gene was located between COL and CH03d11.     

Ectopic overexpression of tomato JERF3 in tobacco activates downstream gene expression and enhances salt tolerance

Plant Molecular Biology - The ethylene, jasmonic acid and osmotic signaling pathways respond to environmental stimuli and in order to understand how plants adapt to biotic and abiotic stresses it...     

Stability study on the nitrile hydratase of Nocardia sp. 108: From resting cells to crude enzyme preparation

In recent years nitrile hydratases (NHases) have drawn increasing attention due to their critical roles in organic synthesis. In the present paper an extensive investigation on the stability and activity of NHase from Nocardia sp. 108, which has succeeded in industrial application in China, was conducted by bioconversion of acrylonitrile to acrylamide in a batch manner. A study of cultivation demonstrated that biosynthesis of NHase changed significantly with the time of the culture, and the optimal NHase biosynthesis phase was 45 h after inoculation with NHase activity of a biomass of 1209.8 U/g. A stability study indicated that both crude enzyme preparations exhibited a good stability when exposed to a pH 7.2 tris-HCl buffer at 4°C for 4 h. The text was submitted by the authors in English.