Evaluation on monkeys of reactogenicity and effectiveness of the complex immunoglobulin preparation formulation

Experiments on 75 monkeys, 20--healthy and 75--with diarrhea, showed that ready medicinal forms of the complex immunoglobulin preparation in tablets, capsules or in a dried form (vials) were safe, well tolerated, clinically and bacteriologically effective. When used simultaneously with antiparasitic treatment, this preparation, irrespective of the kind of its ready medicinal form, showed high curative effect (80-90%). The diarrhea ceasing was accompanied by the gradual normalization of intestinal microbiocenosis (an increased concentrations of Escherichia coli and Bifidobacterium, a decreased amount of opportunistic bacteria). The complex method of the treatment of diarrhea in monkeys (antibiotics, trichopol, phage and probiotics) made it possible to achieve curative effect only in 60% of cases, which required the additional course of treatment, namely the use of rehydration therapy.     

Method of production of the dried highly dispersed vaccine of Newcastle disease virus

Experimental batches of dried dust-like vaccine on the basis of Newcastle disease virus, strain La Sota, have been prepared. The technology of the preparation of the vaccine includes the lyophilization of the technical fluid of the culture under study, milling dried biomass and mixing the pulverized biomass with excipient. As revealed in the process of this work, lyophilization ensures high concentration of the virus in dried biomass and its pulverization, a high content of the target fraction of biomass particles in the vaccine preparation with the moderate inactivation of the bioagent. By mixing the pulverized biomass with excipient the required concentration of the viable virus in the finished product may be achieved. The viability of the bioagent during the storage of the dried vaccine under conditions, determined by optimum storage temperature and time, has been found satisfactory.     

Production of stable Bacillus thuringiensis suspensions in an electromagnetic apparatus

The experiment demonstrated the podibility of obtaining stable suspensions of B. subtilis, a microorganism used as an experimental model, in an electromagnetic apparatus with a bilateral inductor. The optimum conditions of obtaining such suspensions were chosen, these conditions excluding the inactivation of the bacteria used as a model in the course of the process. Suspensions containing 75% of culture fluid were shown to have the highest stability during prolonged storage. Suspensions on the basis of the crystal-forming bacteria B. thuringiensis sp. kurstaki, strain Z-52, used in the production of entomopathogenic preparations and characterized by high stability and good viability in the process of storage were prepared under the selected conditions. The apparatus used in these experiments was recommended for use in the technology of the production of biopreparations of the basis of the above-mentioned bacteria.     

Cultivation of microorganisms in micro-volumes of culture media

Studies on the cultivation of aerobic microorganisms in microdrops of growth medium, immobilized in a layer of highly dispersed disconnector (Aerosil), were carried out. The dispersion of the growth medium and its immobilization in a layer of Aerosil was found to produce no influence on the morphological, cultural and biochemical properties of Serratia marcescens cells, determined both immediately after treatment with the field and some time after storage of the treated suspensions. On the contrary, the duration of the action of ferromagnetic bodies produced essential influence on the viability of the microorganisms in dispersed growth medium and on the accumulation of cells in the course of subsequent cultivation. The development of cells grown in the microdrops of the nutriuent medium was similar to that of cells grown in submerged cultivation, following the S-shaped curve. With the increase of the seed dose a drop in the initial growth rate occurred and the accumulation of microbial cells increased by the end of the process. The greatest increase of biomass was observed with minimal seed doses. With a rise in the concentration of amino nitrogen in the growth medium the yield of cells increased only till this concentration reached 320 mg%. Under these conditions the specific growth rate also increased. The accumulation of cells was greater and occurred earlier when microorganisms were cultivated in immobilized state in powder.     

A hyperefficient process for enzymatic cellulose hydrolysis in the intensive mass transfer reactor

Summary A new type of bioreactor, the intensive mass transfer reactor (IMTR), has been developed for enzymatic hydrolysis of cellulose. Using T. reesei cellulases (2 FPU/ml), 3.5–6% of sugars were obtained in the IMTR after 0.5–2 h of cellulose hydrolysis with a productivity of 30–73 g/l.h.