Dimethyl sulfoxide as a corrector of disorders of cerebral hemodynamics, oxygen and carbohydrate metabolism of the brain and lipid peroxidation in the early period after intracerebral hemorrhage

Intraperitoneal injection of DMSO (0.7 g/kg) in rats 1h after intracerebral hemorrhage prevented brain conjugated diens and fluorosciene products increase and limited cerebral ++anti-radical activity. In experiments on cats 1h after intracerebral hemorrhage intravenous infusion of DMSO (0.3 g/kg) for 30 min improved cerebral hemodynamic, oxygen and glucose metabolism; cats mortality was decreased.     

Spin labels in the analysis of Ca-ATPase in the sarcoplasmic reticulum of rabbit skeletal muscles in hypercholesteremia

The method of electron paramagnetic resonance with spin-labeled maleimide was used to study variation of the structure of Ca-ATPase of the sarcoplasmic reticulum (SR) in rabbit skeletal muscles under long-term hypercholesterolemia (HC). The rate of the maleimide spin label binding with Ca-ATPase of the SR was decreased in HC, which correlated with a lesser access of spin-labeled thiol groups for potassium ferricyanide and sodium ascorbate. HC led to a considerable reduction in the lability and to enhancement of hydrophobia of the spin-labeled fragment of the enzyme. It is concluded that the disordered function of the SR Ca-pump is a consequence of structural changes in the Ca-ATPase molecule in HC.     

Peculiarities of carbohydrate energy and nitrogen metabolism in rat brain affected by magnetic fields of commercial frequency

Carbohydrate oxidation, the coupling of oxidation with phosphorylation and the level of nucleic acids in the brain tissue were studied as affected by variable magnetic fields of commercial frequency (50 Hz) depending on intensity and regime of generation. A repeated effect (15-seances) of the 32 kA/m continuous magnetic field decreases the content of glycogen, creatine phosphate, intensity of oxygen uptake and etherification of inorganic phosphate, glutamine and increases the DNA content. The 7.5 kA/m continuous magnetic field has no effect on the studied metabolic indices in the brain whereas the interrupted field (1s impulse duration, 2s interimpulse interval) of the same intensity and exposition lowers the amount of glycogen, glucose, creatine phosphate, the RNA and DNA exposition lowers the amount of glycogen, glucose, creatine phosphate, the RNA and DNA levels, activates the oxidation processes, intensifies their coupling with phosphorylation.     

Synthesis of nucleosides labeled with tritium at the 5'-carbon atom of the ribose residue

Chemical and enzymatic syntheses of [5'-3H]adenosine, [5'-3H]guanosine, and [5'-3H]uridine have been developed. The reduction of beta-D-ribo-pentadialdo-1,4-furanosyl derivatives of corresponding bases is used in the chemical synthesis. The maximum molar activity of the labelled products was 220 TBk/mol in reactions with [3H]NaBH4 and 370-740 TBk/mol in reactions with gaseous tritium. The enzymatic synthesis was performed by the rebosylation of heterocyclic bases with nucleoside phosphorylase and [5'-3H]uridine as a ribosyl donor. Nucleoside phosphorylase is proposed to be used in the immobilized form to avoid the decrease of molar activity. Nucleosides labelled with tritium both in ribosyl and heterocyclic moieties were synthesised enzymatically.     

Effect of radiation, thermal or combined radiation-thermal damage to a population of progenitor cells of the granulocyte-macrophage series in mouse bone marrow

The recovery of a population of granulopoietic-monocytic colony-forming units of mouse bone marrow was only slightly inhibited during the first two weeks after exposure to a mixture of radiation (4 Gy) and heat (burn of 10 per cent of the skin). In the interval between days 14 and 28 following the effect, their number reached the control level, then it decreased again.     

Comparative study of the immunochemical properties of the 5th lactate dehydrogenase isoenzyme from rat liver and skeletal muscle. II. Isolation and characteristics of LDH-5 preparations from rat liver. General analysis of the results

The method of isolation of chromatographically homogenic LDG-5 preparation from the rat liver was elaborated. The preparations obtained in the present study and LDG-5 preparations from skeletal muscles have a common component which is testified by results of the precipitation test in agar. In the rat liver there is an enzymatically non-active antigenic component, which, according to some of its physico-chemical parameters, is very similar with chemically modified forms of LDG-5 dimers.     

Effect of vasopressin on the ATP-ase activity of microsomal fractions of rabbit heart and liver]

It was shown that intravenous injection of vasopressin in a dose of 5 pressor units per 1 kg of body weight led to changes in the ATP-ase activity of the heart and liver microsomes in one hour. These changes coursed in a different direction, i.e. ATP-ase activity of the heart microsomes increased, and ATP-ase activity of the liver microsomes decreased.