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İncili Çınar Yiğit Arslan Büşra Çelik Esra Nurten Yer Ulu Ferhat Horuz Erdoğan Baloglu Mehmet Cengiz Çağlıyan Ebrar Burcu Gamze Bayarslan Aslı Ugurlu Altunoglu Yasemin Celik 《Protoplasma》2023,260(2):509-527
Protoplasma - Watermelon and melon are members of the Cucurbitaceae family including economically significant crops in the world. The expansin protein family, which is one of the members of the... 相似文献
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Pedro M. D. Moreno Sylvain Geny Y. Vladimir Pabon Helen Bergquist Eman M. Zaghloul Cristina S. J. Rocha Iulian I. Oprea Burcu Bestas Samir EL Andaloussi Per T. J?rgensen Erik B. Pedersen Karin E. Lundin Rula Zain Jesper Wengel C. I. Edvard Smith 《Nucleic acids research》2013,41(5):3257-3273
In spite of the many developments in synthetic oligonucleotide (ON) chemistry and design, invasion into double-stranded DNA (DSI) under physiological salt and pH conditions remains a challenge. In this work, we provide a new ON tool based on locked nucleic acids (LNAs), designed for strand invasion into duplex DNA (DSI). We thus report on the development of a clamp type of LNA ON—bisLNA—with capacity to bind and invade into supercoiled double-stranded DNA. The bisLNA links a triplex-forming, Hoogsteen-binding, targeting arm with a strand-invading Watson–Crick binding arm. Optimization was carried out by varying the number and location of LNA nucleotides and the length of the triplex-forming versus strand-invading arms. Single-strand regions in target duplex DNA were mapped using chemical probing. By combining design and increase in LNA content, it was possible to achieve a 100-fold increase in potency with 30% DSI at 450 nM using a bisLNA to plasmid ratio of only 21:1. Although this first conceptual report does not address the utility of bisLNA for the targeting of DNA in a chromosomal context, it shows bisLNA as a promising candidate for interfering also with cellular genes. 相似文献
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Alptekin Burcu Erfatpour Mohammad Mangel Dylan Pauli Duke Blake Tom Turner Hannah Lachowiec Jennifer Sherman Jamie Fischer Andreas 《Molecular breeding : new strategies in plant improvement》2022,42(10):1-15
Molecular Breeding - Maize amylose is a type of high value-added starch used for medical, food, and chemical applications. Mutations in the starch branching enzyme (SBEIIb), with recessive ae... 相似文献
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Reconstruction of the weight-bearing surface of the foot with nonneurosensory free flaps 总被引:3,自引:0,他引:3
Sönmez A Bayramiçli M Sönmez B Numanoğlu A 《Plastic and reconstructive surgery》2003,111(7):2230-2236
Neurotized fasciocutaneous flaps and split-skin grafted muscle flaps are the most frequently used free flap alternatives for the reconstruction of weight-bearing surfaces of the foot. An objective comparison of the innate characteristics of these two flap types, with respect to long-term stability, has not been possible because sensory reinnervation in the fasciocutaneous flaps has been a confounding factor. This study compares nonsensate fasciocutaneous flaps (n = 9) with nonsensate split-skin grafted muscle flaps (n = 11), with mean follow-up periods of 34.3 and 31.3 months, respectively. Patients completed a form that included questions regarding degree of pain at the operative site, presence of ulcers, ability to wear normal shoes, employment status, and time spent standing on foot. Touch and deep sensation were evaluated with Semmes-Weinstein and vibration tests, respectively. Significantly less pain and less ulceration (p < 0.05) were observed in the fasciocutaneous group. Semmes-Weinstein monofilament tests revealed poorer results with split-skin grafted muscle flaps, compared with fasciocutaneous flaps. These results indicate that even if the sensory protection of fasciocutaneous flaps is not considered, these flaps have superior properties, compared with split-skin grafted muscle flaps. 相似文献
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Burcu Kaplan-Türköz Thomas Koelblen Christine Felix Marie-Pierre Candusso David O’Callaghan Annette C. Vergunst Laurent Terradot 《FEBS letters》2013
BtpA/Btp1/TcpB is a virulence factor produced by Brucella species that possesses a Toll interleukin-1 receptor (TIR) domain. Once delivered into the host cell, BtpA interacts with MyD88 to interfere with TLR signalling and modulates microtubule dynamics. Here the crystal structure of the BtpA TIR domain at 3.15 Å is presented. The structure shows a dimeric arrangement of a canonical TIR domain, similar to the Paracoccus denitrificans Tir protein but secured by a unique long N-terminal α-tail that packs against the TIR:TIR dimer. Structure-based mutations and multi-angle light scattering experiments characterized the BtpA dimer conformation in solution. The structure of BtpA will help with studies to understand the mechanisms involved in its interactions with MyD88 and with microtubules. 相似文献
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Banoglu E Çalişkan B Luderer S Eren G Özkan Y Altenhofen W Weinigel C Barz D Gerstmeier J Pergola C Werz O 《Bioorganic & medicinal chemistry》2012,20(12):3728-3741
Pharmacological suppression of leukotriene biosynthesis by 5-lipoxygenase (5-LO)-activating protein (FLAP) inhibitors is a promising strategy to intervene with inflammatory, allergic and cardiovascular diseases. Virtual screening targeting FLAP based on a combined ligand- and structure-based pharmacophore model led to the identification of 1-(2-chlorobenzyl)-2-(1-(4-isobutylphenyl)ethyl)-1H-benzimidazole (7) as developable candidate. Compound 7 potently suppressed leukotriene formation in intact neutrophils (IC(50)=0.31 μM) but essentially failed to directly inhibit 5-LO suggesting that interaction with FLAP causes inhibition of leukotriene synthesis. For structural optimization, a series of 46 benzimidazole-based derivatives of 7 were synthesized leading to more potent analogues (70-72, 82) with IC(50)=0.12-0.19 μM in intact neutrophils. Together, our results disclose the benzimidazole scaffold bearing an ibuprofen fingerprint as a new chemotype for further development of anti-leukotriene agents. 相似文献
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Kurtoğlu S Sen B Melikoğlu G Becker H Zapp J Kiemer AK Meriçli F Meriçli AH 《Zeitschrift für Naturforschung. C, Journal of biosciences》2012,67(3-4):103-107
From the roots of Aconitum vulparia Rchb., collected in Prüm (Germany), a new norditerpenoid alkaloid, named alexhumboldtine, has been isolated along with the known norditerpenoid alkaloids lappaconitine, anthranoyllycoctonine, lycoctonine, puberaconitine, ajacine, and septentriodine. The structure of alexhumboldtine was established on the basis of 1H, 13C, DEPT, homonuclear 1H COSY, NOESY, HSQC, and HMBC NMR studies. From the aerial parts of the plant another norditerpenoid alkaloid, aconorine, has been isolated. 相似文献