4'-C-modified nucleosides: synthesis and antiviral properties

The synthetic methods for 4'-C-modified nucleosides as well as structure activity relationship of obtained compounds towards hepatitis C virus are reviewed.     

PEGylated recombinant L-asparaginase from <Emphasis Type="Italic">Erwinia carotovora</Emphasis>: Production,properties, and potential applications

N-hydroxysuccinimide ester of monomethoxy polyethylene glycol hemisuccinate was synthesized. It acylated amino groups in a molecule of recombinant L-asparaginase from Erwinia carotovora. A method of L-asparaginase modification by the obtained activated polyethylene glycol derivative was developed. The best results were produced by modification of the enzyme with a 25-fold excess of reagent relative to the enzyme tetramer. The modified L-asparaginase was isolated from the reaction mixture by gel filtration on Sepharose CL-6B. The purified bioconjugate did not contain PEG unbound to the protein, demonstrated high catalytic activity, and exhibited antiproliferative action on cell cultures.     

The experiment “initial stages of biological damage and deterioration in space”

A study of the microflora of structural surfaces on board the russian segment of the international space station (RS ISS) has demonstrated that the species composition that is formed during the mission is similar to that on board the mir station. the microflora of the sampled sites was similar to that of residential and industrial buildings on earth. it was represented by 17 species of fungi, seven genera of bacteria, and three genera of actinomycetes. the degree of colonization of the surfaces of structural materials of the rs iss by destructive microorganisms was low and showed undulating dynamics. the mycelial fungi included “technophile” species, which caused biological corrosion of an aluminum-magnesium alloy in simulation experiments.     

Development of a latex agglutination method for the diagnosis of Pseudomonas aeruginosa infection

The present investigation has revealed the possibility of using different kinds of monodispersed polystyrene latex, produced in the USSR, as carriers in the process of the preparation of antibody diagnosticums intended for the detection of water-soluble slime antigens of Pseudomonas aeruginosa strains belonging to the most widespread serological types. The optimum conditions for the preparation of latex reagents and for making the latex-agglutination test have been experimentally established. The new diagnosticums+ have been shown to be highly species- and type-specific, which permits making judgment on the presence or absence of slime antigens of P. aeruginosa strains belonging to definite serovars in the clinical material under study. The preparations thus obtained have been found to retain their sensitivity for 16 months (the term of observation).     

Contact interactions of spreading thrombocytes

Contact interactions of rabbit platelets was studied in the course of their spreading over the siliconized glass surface. When the density of cells adhered to the substratum surface is high enough, the platelets are spreading in such a way that their edges are in a close proximity, while the overlapping of cells is very rare and scarcely pronounced. A morphometric analysis of the probability of such an organization of platelets at their random and independent disposition on the substratum proves the existence of a contact inhibition of platelets spreading on the substratum. Thus, a phenomenon of the contact inhibition seems to be a common feature not only of large nucleated cells but also of small anuclear platelets.     

Silver in the meat and organs of broiler chickens in case of using colloidal silver as an alternative to antibiotics

The search for new antibacterial products, the mechanisms of action of which differ from conventional antibiotics is a current a topical issue. The objective of our research is to identify the presence of silver in meat and organs of broiler chicks that had been given colloidal silver. The results show that the broiler chick meat contains silver in quantities safe for humans regardless of the use of colloidal silver. Comparison of meat analysis results in experimental and control groups indicate that the ratio of parameters distribution variance for all birds to the mean variance by group for each measured no statistical differences in the chemical composition of bird’s meat of experimental and control groups. The analysis also confirmed the existing difference in chemical composition of leg muscle meat and chest muscle meat (P?<?0.05), whereas leg muscle contains more fat (6.81% vs. 2.85%) and less protein (20.25% vs. 22.81%).     

A neurophysiologic analysis of the effect of amidopyrine at the thalamo-cortical level of the somatosensory system]

Acute experiments were conducted on nonanesthetized cats; it was shown that amidopyrin (10, 20 mg/kg, intravenously) decreased the level of the background activity of the thalamic neurons, and, at the same time, increased the amplitude of the focal primary bioelectrical reactions. It is supposed that amidopyrin synchronized evoked reactions of the cells in the thalamus, as well as in the cortex. On the other hand, amidopyrin diminished the excitability of the cortical neurons participating in the interzonal connections.