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1.
2.
G Carloni A M Venuat L Daya-Grosjean P Nardeux J S Rhim B Azzarone 《FEBS letters》1988,229(2):333-339
The human osteosarcoma cell line Te85 clone F-5 is not tumorigenic in vivo. Its transformation with Kirsten murine sarcoma virus (KiMSV) (KHOS) confers full malignant properties and stable non-tumorigenic revertants of this KHOS cell line have been obtained. Here we show that integration and expression of a single copy of the KiMSV proviral DNA, which is totally lost in the HOS 240S revertant, is responsible for the acquisition of tumorigenicity. Cytogenetic analysis and the absence of a residual LTR copy in the revertant cellular genome suggest that the loss of KiMSV provirus is caused either by chromosomal segregation or by recombination not involving the LTR. In addition analysis of the expression of ras proteins revealed no changes in the pattern of c-ras products and the expression of v-ras only in the KHOS cells. All these data suggest that Te85 and HOS 240S cell lines could represent a human alternative recipient system to rodent cells in studies with oncogenes. 相似文献
3.
C Saint-Ruf P Nardeux S Estrade D Brouty-Boye C Lavialle J S Rhim R Cassingena 《Experimental cell research》1988,176(1):60-67
The human epithelial HBL-100 cell line harbors SV40 genetic information and has an unlimited growth potential. Despite displaying properties characteristic of transformation since its early in vitro passages, it is capable of producing progressively growing tumors in nude mice only after long-term culture. This is a reproducible phenomenon and apparently not the consequence of a selection of preexisting malignant cells. Superinfection of early passage nontumorigenic HBL-100 cells with Kirsten murine sarcoma virus, which contains a Ki-ras oncogene having undergone multiple activating events, induces morphologic alterations and rapidly converts the cells to neoplastic cells, further supporting the hypothesis of multistep carcinogenesis. The HBL-100 cell line might be useful in defining the oncogenes representative of different families, which are able to complement SV40 in this system. 相似文献
4.
Summary The N-metabolism ofArthrocnemum fruticosum (L.) Moq., growing in a saline area north-east of the Dead Sea in Jordan, was studied over its vegetative growth period from
March to September 1981. Plant and soil samples were taken at monthly intervals. Water content, Na+, K+, Cl−, NH
4
+
, NO
2
−
and NO
3
−
concentrations were determined in the soil extracts, and the same determinations plus ash weight, soluble carbohydrates,
proline, proteins andin vivo nitrate reductase in the plant roots and shoots.
Soil humidity decreased and salinity increased from March to August, with re-wetting occurring in late July. K+ and Cl− were much lower in the soils than Na+. Plant relative dry weight increased during summer due to the absorption of Na+ in addition to increased organic dry weight. The uptake of Na+ was not balanced by a similar uptake of Cl−.
Ammonium and nitrate decreased in soil and plants in parallel with increasing salinity. Nitrite was only found in the roots
and always in very low quantities. Proline was found only in March. The total soluble carbohydrates in the roots showed a
short increase in June when the sodium in the plants also increased. It was concluded that carbohydrates may be used to balance
osmotic shocks, but that another compatible compounds is necessary to maintatin long-term osmotic equilibrium.
The nitrate reductase activity, measuredin vivo, and the soluble protein changed roughly in parallel with the internal nitrate from May to August, suggesting that nitrogen
uptake and reduction in the plant is inhibited during summer when the soil is dry and very saline. This could be a direct
effect of drought and/or salinity on the plants, or an indirect onevia an inhibition of nitrifying bacteria. 相似文献
5.
Seong-Lyul Rhim Hwa-Jin Cho Byung-Dong Kim Wolfgang Schnetter Klaus Geider 《Molecular breeding : new strategies in plant improvement》1995,1(3):229-236
A crystal -endotoxin gene ofBacillus thuringiensis subsp.tenebrionis (B.t.t.) encoding a coleopteran insect-specific toxin was used to construct a chimeric gene which expressed the toxin in plant cells. Via anAgrobacterium tumefaciens binary vector system, the toxin gene was transferred into tomato cells. From leaf disks recombinant plants were regenerated. Hybridization experiments demonstrated that these plants synthesized toxin-specific mRNA of the expected size. Transgenic tomato plants with the chimericB.t.t. toxin gene contained a 74 kDa protein which cross-reacted with toxin antibodies. The expression caused a significant insecticidal activity of the transgenic tomato plants against Colorado potato beetle larvae. 相似文献
6.
D. P. Chora L. Reddy S. K. Gupta L. Wan P. A. Mathieu R. L. Shoemaker J. S. Rhim 《In vitro cellular & developmental biology. Animal》1994,30(8):539-546
Summary Cystic fibrosis (CF) involves abnormalities in mucus production and secretion of the airway. Studies of the regulation of
airway mucin production and secretion has been difficult due to the lack of in vitro models of the airway epithelial cells
which express functional differentiation. Because the majority of the mucin in the airway is apparently produced by the submucosal
glands, we have focused our attention on the development of cell culture models of human airway submucosal glands. This report
describes the propagation of CF airway submucosal gland epithelial cells which continue to express mucin production. The CF
bronchus was obtained from a 31-yr-old patient who received a double lung transplant. The glands were dissected out and primary
cultures prepared by the explant/outgrowth procedure. The cells were immortalized by infection with Adl2-SV40 hybrid virus.
The cultures are maintained in serum-free keratinocyte basal medium supplemented with insulin (5μg/ml), hydrocortisone (0.5μg/ml), epidermal growth factor (10 ng/ml), bovine pituitary extract (25μg/ml), and antibiotics. Cultures were passaged using 0.125% trypsin in Ca+2 and Mg+2-free Hanks’, balanced salt solution. Polymerase chain reaction (PCR) analysis demonstrated that the cells were homozygous
for the ΔF508 mutation. Morphologic observations showed that the cells were epithelial and were interconnected by sparsely
distributed desmosomes. Their cytoplasm contained secretory-type structures including abundant Golgi, rough endoplasmic reticulum,
and secretory vesicles. Immunofluorescent studies determined that all cells were positive for cytokeratins, mucin glycoconjugates,
and cystic fibrosis transmembrane conductance regulator. The cultures secreted substantial amounts of mucin glycoproteins
and expressed the MUC-2 mucin gene. Patch clamp experiments revealed that the cells expressed defective Cl− channels which were not activated by Forskolin. 相似文献
7.
Citrus Tristeza Virus (CTV), usually occurs in nature as a mixture of genotypes. Six naturally infected citrus
(Citrus sinensis) trees grafted on sour orange rootstock were collected from three citrus growing governorates
in Egypt (Sharqia, Qalyubia and Garbia). In this study, RT-PCR, Single-Strand Conformation Polymorphism
(SSCP) and nucleotide sequence analysis were used for four independent CTV genomic regions (p65, p18,
p20, and p23) to detect and assess the sequence and genetic variabilities among CTV Egyptian isolates. RTPCR products (650 bp) for the CTV p23 gene obtained from the selected isolates were used for the SSCP analysis
and DNA sequencing. SSCP patterns of p23 gene for individual isolates yielded different complex haplotype patterns. Nucleotide sequence analysis of p23 region amplified from six isolates under study revealed that p23 shared
high nucleotide identity 98.7% with T36 isolate from USA, Florida. Phylogenetic analysis of p23 gene indicated a
close evolutionary relationship between all examined isolates and Qaha isolate (T36 isolate group), suggesting that
they may have originated from closely related ancestors. Nucleotide sequence analysis of the three genes located
on CTV 3′-coterminal overhang, p18, p20 and p65, amplified from isolate A3, Sharqia governorate, revealed that
the p18, p65, and p20 genes were related to the T3-KB isolate from South Africa with 99%–100% sequence
homology. Phylogenetic relationship analysis for p65, p18 and p20 ORFs clustered the current A3 isolate with
T3 genotype group. The recombination analysis identified three of six isolates from Sharqia, and Garbia as potential recombinant for p23 gene. The isolates T36 and T3 were identified as major donors for recombination events
in isolate A3. Our results concluded that p23 ORF likely to be as a hotspot region for recombination and originated through recombination event. The current study indicated that recombination is an important factor for the
origin of CTV strains in Egypt. 相似文献
8.
We have examined the effect of 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) on mitogen-stimulated growth and on c-myc proto-oncogene expression in a keratinocyte model of tumor progression. A dose-dependent inhibition of cell growth by 1,25-(OH)2D3 was demonstrated in both established (HPK1A) and malignant (HPK1A-ras) cells. However, this inhibition was observed with the addition of 1,25-(OH)2D3 at a higher concentration in HPK1A-ras cells than in HPK1A cells. Cell cycle analysis revealed a blockage of the normal progression of the cell cycle from G0 to S phase in the presence of 1,25-(OH)2D3. A higher concentration of 1,25-(OH)2D3 was required in HPK1A-ras cells to overcome the mitogen-stimulated progression into S phase, when compared with HPK1A cells. Analysis of c-myc messenger RNA revealed a strong inhibition of its expression at early time points with higher concentrations of 1,25-(OH)2D3 being required to obtain an inhibition in HPK1A-ras cells similar to that obtained in HPK1A cells. 1,25-(OH)2D3 receptor characterization by sucrose gradient analysis and equilibrium binding demonstrated the presence of a single 3.7 S protein with similar receptor numbers and affinity in both cell lines. These observations therefore demonstrate that an alteration of the growth inhibitory response to 1,25-(OH)2D3 occurs when keratinocytes acquire the malignant phenotype and suggest that the alteration lies beyond the interaction of the ligand with its receptor. In addition, relative resistance to 1,25-(OH)2D3 was also observed in the expression of the cell-cycle associated oncogene c-myc. These studies may therefore have important implications in vivo in the development and growth of epithelial cell cancers. 相似文献
9.
Ming-Chi Wu Grace K. Arimura John S. Holcenberg Adel A. Yunis 《In vitro cellular & developmental biology. Plant》1982,18(9):750-754
Summary Cultured human pancreatic carcinoma cells (MIA PaCa-2) have been shown previously to be very sensitive toE. coli
l-asparaginase (EC II). The present studies have demonstrated that another enzyme,Acinetobacter glutaminase-asparaginase (AGA) is much more effective in inhibiting cell growth. At the concentration of 0.0025 U/ml of AGA
activity the enzyme totally inhibited cell growth, whereas the EC II with the same concentration did not show any effect.
The inhibition of cell growth correlated well with inhibition of protein and glycoprotein synthesis. The addition ofl-glutamine at the concentration of 1 mM completely reversed the inhibition of protein synthesis. Similarly, the addition ofl-glutamine at the concentration of 3 mM daily on 3 successive days after adding AGA resulted in significant reversal of growth inhibition. The results of this study
indicate that the action of AGA on MIA PaCa-2 is, to a great extent, exerted through itsl-glutaminase activity.
This work was supported in part by USPHS Grant CA 19182. Dr. Wu is recipient of Research Career Development Award Grant CA00686
and Dr. Yunis is a Howard Hughes Investigator. 相似文献
10.