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Mohammed-Said Bouaouda Filippo Barattolo Abdelmalek El Kamar 《Revue de Micropaléontologie》2009,52(2):107
The recent biostratigraphic and micropaleontologic works permitted to specify the distribution of Megaporellaboulangeri Deloffre and Beun in the Jurassic of the Moroccan Atlantic basin. The lower limit of the extension of this dasycladale alga, earlier attribued to the Early Kimmeridgian by Deloffre and Beun (1986), is currently placed in the Early Callovian, on the basis of the age provided by Megaporellaboulangeri biozone. The Otternstellaarabica biozone dates the upper limit of the extension of the species of the Early Oxfordian. Megaporellaboulangeri proliferates in the borders of the basin where the internal lagoons facies dominate, however it is absent in the western parts with open sea facies. 相似文献
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Toxin-based in-vitro selection and its potential application to date palm for resistance to the bayoud Fusarium wilt 总被引:1,自引:0,他引:1
El Hadrami A El Idrissi-Tourane A El Hassni M Daayf F El Hadrami I 《Comptes rendus biologies》2005,328(8):732-744
Date palm (Phoenix dactylifera L.) is qualified as a 'tree' of great ecological and socio-economical importance in desert oases. Unfortunately, it is being decimated, especially in Morocco and Algeria, by a fusariosis wilt called bayoud and caused by Fusarium oxysporum f. sp. albedinis (Fao). Controlling this disease requires the implementation of an integrated management program. Breeding for resistance is one of the most promising component strategies of this program. Few naturally resistant cultivars with a mediocre fruit quality (dates) are known. Conventional and non-conventional methods are under development and have to use the simplest and easiest methods to screen for resistant individuals. The use of pathogen toxins as selective agents at the tissue culture step might be a source of variability that can lead to the selection of individuals with suitable levels of resistance to the toxin and/or to the pathogen among the genetic material available. Foa produces toxins such as fusaric, succinic, 3-phenyl lactic acids and their derivatives, marasmins and peptidic toxins. These toxins can be used bulked or separately as selective agents. The aim of this contribution was to give a brief overview on toxins and their use as a mean to select resistant lines and to initiate a discussion about the potential use of this approach for the date palm-Foa pathosystem. This review does not pretend to be comprehensive or exhaustive and was prepared mainly to highlight the potential use of Foa toxins for selecting date palm individuals with a suitable resistance level to bayoud using toxin-based selective media. 相似文献
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Denis O Van Cauwenberge A Treutens G Es Saadi B Symoens F Popovic N Huygen K 《Mycopathologia》2012,173(2-3):151-162
In this study, three different rat hybridoma cell lines secreting monoclonal antibodies (mAbs) recognizing the spores from Alternaria alternata, a plant pathogenic fungus, contaminant of food products and important cause of both allergic rhinitis and asthma, have been characterized. These three mAbs are all of IgM isotype. Two antibodies, A1 and F10, were cross-reactive antibodies recognizing spores from Alternaria, Cladosporium, Penicillium, Aspergillus and Stachybotrys genera, but not the yeasts Saccharomyces cerevisiae or Candida albicans. Competitive and sandwich assays demonstrated that these two mAbs were directed against the same or very close repetitive(s) epitope(s). A1-based sandwich ELISA efficiently detected this epitope in various mould (but not yeast)-soluble extracts prepared from strains grown in the laboratory. Moreover, this A1-based sandwich ELISA detected its cognate epitope in air and dust samples obtained from dwellings. The third antibody, E5, recognized only the spores of Alternaria and the phylogenetically very close Ulocladium botrytis. This E5 antibody is directed against a repetitive epitope found in Alternaria and Ulocladium laboratory extracts and can be used in a sandwich assay for the quantification of these moulds. Therefore, E5 antibody is a promising tool for the development of Alternaria-Ulocladium-specific immunoassays, while A1 and F10 could be interesting tools for the quantification of the total mould biomass. 相似文献
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Alexandre Berr Emily J. McCallum Abdelmalek Alioua Dimitri Heintz Thierry Heitz Wen-Hui Shen 《Plant physiology》2010,154(3):1403-1414
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Chabchoub N Abdelmalek R Breton J Kanoun F Thellier M Bouratbine A Aoun K 《Parasite (Paris, France)》2012,19(2):147-151
The microsporidian species Enterocytozoon bieneusi is a major cause of chronic diarrhea and malabsorption in patients with AIDS. Genotyping was performed on seven E. bieneusi strains for the first time in Tunisia. All the strains were isolated from stool samples of humans with immunodeficiency virus (HIV) infection. Analysis of the ribosomal RNA gene internal transcribed spacer (rDNA ITS) allowed the identification of three distinct genotypes previously described in other studies. Genotypes D and B were characterized in four and two respectively. The Peruvian genotype (Peru 8) was detected in the last isolate. These results indicate a genetic diversity in E. bieneusi strains from HIV Tunisian patients and suggest the coexistence of both zoonotic and anthroponotic route of transmission. 相似文献
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Amel Adel Emmanuel Abatih Niko Speybroeck Abdelkrim Soukehal Rachid Bouguedour Karim Boughalem Abdelmalek Bouhbal Mouloud Djerbal Claude Saegerman Dirk Berkvens 《PloS one》2015,10(3)
A large-scale study on canine Leishmania infection (CanL) was conducted in six localities along a west-east transect in the Algerian littoral zone (Tlemcen, Mostaganem, Tipaza, Boumerdes, Bejaia, Jijel) and covering two sampling periods. In total 2,184 dogs were tested with an indirect fluorescent antibody test (IFAT) and a direct agglutination test (DAT). Combined multiple-testing and several statistical methods were compared to estimate the CanL true prevalence and tests characteristics (sensitivity and specificity). The Bayesian full model showed the best fit and yielded prevalence estimates between 11% (Mostaganem, first period) and 38% (Bejaia, second period). Sensitivity of IFAT varied (in function of locality) between 86% and 88% while its specificity varied between 65% and 87%. DAT was less sensitive than IFAT but showed a higher specificity (between 80% and 95% in function of locality or/and season). A general increasing trend of the CanL prevalence was noted from west to east. A concordance between the present results and the incidence of human cases of visceral leishmaniasis was observed, where also a maximum was recorded for Bejaia. The results of the present study highlight the dangers when using IFAT as a gold standard. 相似文献
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Role of dexamethasone and insulin on the development of the five urea-cycle enzymes in cultured rat foetal hepatocytes. 总被引:2,自引:1,他引:1 下载免费PDF全文
The activity changes of the urea-cycle enzymes were monitored in cultured foetal hepatocytes after dexamethasone and insulin treatments. Addition of dexamethasone induced the development of carbamoyl-phosphate synthetase, argininosuccinate synthetase, argininosuccinase and arginase activities as soon as day 16.5 of gestation. When insulin was added together with dexamethasone, it markedly inhibited the steroid-induced increase in carbamoyl-phosphate synthetase, argininosuccinate synthetase and argininosuccinase activities. 相似文献
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