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XUSHIXIONG 《Cell research》1993,3(1):93-101
The pattern of change of the microtubule cytoskeleton of the root-tip cells of rice during mitosis was studied using immunofluorescence technic and confocal laser scanning microscopy. All the major stages of ceil division including preprophase, prophase, metaphase, anaphase and telophase were observed. The most significant finding was that in the preprophase cells microtubules radiating from the nuclear surface to the cortex were frequently seen. During development these microtubules became closely associated with the preprophase band and prophase spindie indicating that the microtubules radiating from the nuclear surface, the preprophase band and the prophazc spindle were structurally and functionally closely related to each other. Granule-like anchorage sites for the radiating microtubules at the muclear surface were often seen and the possibility that these gramle-like anchorage sites might represent the microtubule organizing centres was discussed. 相似文献
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用月桂酸对人红细胞超氧化物歧化酶进行化学修饰处到酰化h-SOD,并对Ac-hSOD和h-SOD的稳定性进行了比较。结果表明:Ac-hSOD活力为h-SOD的72%,比活力为4000U/mg,Ac-hSOD的热稳定性,酸碱稳定性及抗蛋白酶水解能力均比天然酶提高。 相似文献
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Zhang Jintun 《生物数学学报》1996,(4)
IIntroductionSuccessionstudyIsaspeclalfleldInvegetationecology(Greig—Smith1983).ItreferstocummulatlvechangeInvegetationovertime.Florl幻IcgradientsIndicativeofsuccessionaltrendsmayemergefromconventionalordinationIfextrinsic!nformatlonallowsthemtobel-dentlfledassuch.Inthespedalcaseofforestvegetation,forInstance,Informationonthesize—classesoftreespedespresentmaypermitsuccessionalgradientslobeldentlfleddirectly(Goff1968,Carleton&Maycock1978).Fewauthors,however,haveattempted… 相似文献
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ZHANG Jinzhu 《中国科学:生命科学英文版》1997,40(5):488-495
Changes of sodium ionic concentration of human erythrocytes applied to pulsed electrical field (PEF) were studied by using shift reagent and NMR spectroscopy. The results show that the concentration of intracellular Na+ increases with the increasing intensity of PEF when the erythrocytes are applied to PEF with higher intensities. The relationship between intracellular Na concentrations and the intensities of PEF does not follow linear or exponen-tial behavior. As the intensities increase, the intracellular Na+concentrations increase even faster by an exponential curve. However under effects of PEF at lower intensities, intracellular Na+ concentration decreases. Ouabain can in-hibit the decrease of intracellular Na concentration, and the inhibition increases with the increasing concentration of ouabain, suggesting that Na+ , K+ -ATPase on cell membrane can be activated by PEF at lower intensities. Direct measurement of activities of the enzyme by using Malachite green method has confirmed this observation. Cell perme-abilities to ions, activation of enzymes by electrical fields and transmission of physical signals like PEF across cell mem-branes are discussed. 相似文献
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槲皮素具有诱导细胞自噬、抑制肿瘤细胞增殖等抗癌功能,但其诱导细胞自噬的分子机制还不太清楚. 本文通过激光共聚焦显微镜观察槲皮素对Hep G2细胞自噬的影响; Fluo-3 AM和Cyto-IDTM Green Detection Reagent染色标记, 流式细胞术测定了槲皮素对Hep G2细胞内游离钙离子浓度[Ca2+]i 及Ca2+螯合剂BAPTA-AM对自噬水平的影响. 探讨了槲皮素诱导人肝癌细胞 Hep G2自噬过程中[Ca2+]i的变化. 结果表明, 在槲皮素较低浓度范围内(0 ~ 50 μg/mL), 可明显抑制Hep G2细胞增殖, 并以剂量依赖方式诱导细胞自噬. 同时发现,槲皮素刺激Hep G2细胞可使[Ca2+]i明显增加, 进而促进自噬. 而当胞内Ca2+螯合剂 BAPTA-AM存在时, 细胞的自噬水平受到一定的抑制. 这些结果表明,细胞内[Ca2+]i的升高可促进自噬, [Ca2+]i 的降低可能会抑制自噬. Hep G2细胞自噬与细胞内游离钙离子浓度的变化有关系. 相似文献
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化学方法合成是新药研发的一种重要途径。结合抗肿瘤药物的作用机制以及蒽醌类衍生物的构效关系,设计合成了一类新的蒽醌类衍生物1-硝基-2-酰基蒽醌-缬氨酸(简称C3),发现其具有很好的抗肿瘤活性。为了确定蒽醌类衍生物C3对结肠癌HCT116和HT29细胞的作用及其分子机制,首先通过MTT比色法检测C3对结肠癌HCT116和HT29细胞活性的影响。结果显示,C3对这两种结肠癌细胞具有明显的抑制作用,呈时间和剂量依赖性。60μg/mL的C3处理HCT116和HT29细胞48 h,细胞活性分别是50.67%和59.77%,达到了半抑制浓度;同时,其细胞形态和细胞核发生明显变化。进一步采用Western印迹和qRT-PCR技术,检测C3对DNA切除修复交叉互补1(excision repair cross-complementation group 1,ERCC1)转录水平和蛋白质水平表达及其稳定性的影响。结果表明,C3降低了ERCC1转录水平和蛋白质水平的表达,并且减弱了ERCC1转录水平和蛋白质水平的稳定性。最后,用U0126(MEK1/2抑制剂)和C3联合作用结肠癌HCT116和HT29细胞,通过Western印迹检测ERCC1蛋白质水平的表达。结果表明,C3通过降低p-ERK1/2的蛋白质水平的表达,从而抑制ERCC1的表达。上述结果证明,C3通过细胞外调节蛋白激酶(extracellular regulated protein kinases, ERK1/2)信号通路,降低了ERCC1转录水平和蛋白质水平的稳定性,使ERCC1转录水平和蛋白质水平表达发生下调,进而抑制结肠癌HCT116和HT29细胞的活性。 相似文献
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