耳叶牛皮消的全长转录组测序及其重要活性物质代谢通路的分析

耳叶牛皮消(Cynanchum auriculatum)为萝藦科(Asclepiadaceae)植物,具有较高的营养价值和药用潜力,是中国传统的药食同源兼用植物。当前耳叶牛皮消的相关研究以活性物质的提取和分类为主,但分子水平的研究较少。本研究借助PacBio三代测序平台,对‘滨乌1号’耳叶牛皮消的转录组进行测序,获得高质量Unigenes共42 710条,总长度为113 782 167 bp。将上述Unigenes与Nr、KEGG、GO、COG、Swissprot、Pfam等数据库比对,分别获得41 642、19 502、21 997、31 150、37 710、37 433条注释,其中在“氨基酸代谢”(1 967条)、“脂质代谢”(1 503条)、“萜类化合物和聚酮化合物代谢”(350条)等通路的注释有助于揭示耳叶牛皮消的药理物质代谢机制。进一步分析表明,类黄酮合成通路上可注释到29条Unigenes,黄酮和黄酮醇合成通路上可注释到2条Unigenes;类固醇合成通路上可注释到68条Unigenes,油菜素类固醇合成通路上可注释到12条Unigenes。通过这些潜力基因的鉴定,可以为...     

Laser-induced tobacco protoplast fusion

Laser tweezers can manipulate small particles, such as cells and organdies. When coupling them with laser microbeam selective fusion of two tobacco protoplasts containing some chloroplast was achieved. Physical and biological variables that affect laser trapping and laser-induced fusion were also discussed. The results show that the effect of chloroplast content and distribution on the yield of cell fusion is remarkable.