Distribution of sulphur forms in soils from beech and spruce forests of Mont Lozère (France)

From a quantitative inventory of sulphur forms and sulphur budget, the relation between the distribution of the various sulphur forms and the sulphate fluxes in three soil profiles has been addressed. These profiles are located in two forested watersheds at Mont Lozère. One has been sampled in a beech forest and the other two in a spruce forest and in a harvested plot of this spruce forest, respectively. The mean annual input-output budgets showed a sulphur immobilization in the soil cover of the three plots. In the preserved spruce forest plot, because of larger dry depositions, the sulphur immobilization is much greater than in other plots and occurs essentially in the B horizons. In the other two profiles, the dominant immobilization occurs in the parent material.The total sulphur content is very high in the forest floor reaching 2065 g S g^-1 in the litter of one of the soils under spruce. In the organo-mineral horizons of soils under spruce, the total sulphur content decreases with depth and ranges from 310 to 520 g S g^-1 in the A horizons to 100–200 g S g^-1 in the parent material. In the profile under beech, the total sulphur content is lower except in the parent material. In all cases, the organic sulphur is the major part of sulphur often representing more than 90% of total sulphur. In organo-mineral soil horizons of the spruce forest, the part of the sulphateesters is more important than in the soil of the beech forest, probably related to the different nature of the microbial activity in the spruce forest. In contrast, the humification processes are more efficient in the soil under beech, which can be due to the greater input of organic sulphur by litterfall. It appears that the dominant organic sulphur form varies as a function of microbial ecology and sulphate flux. The maximum of the inorganic sulphate is located at the base of the B horizons in the soil of the spruce forest and in the parent material of the soil under beech. In these horizons, the high content of inorganic sulphate can be related to the higher amounts of amorphous Fe and Al phases.     

Early metabolic changes in regenerating microfragments of the liverwort Riella helicophylla (Bory et Mont.) Mont.: Protein and RNA synthesis,free α-amino concentration

Microfragments of constant size were isolated from the thallus of Riella by a rapid punching procedure. Thus it was possible to study various metabolic parameters especially during the first hours after fragment isolation. Protein synthesis increases rapidly after a slight decrease at 30 min. The earliest significant increase in RNA synthesis occurs at 8 h, indicating a different activation pattern. The concentration of -amino compounds drops at 30 min and then increases continuously, thus exhibiting a close relationship with the measured alterations in protein synthesis. Another indication of metabolic conversions during regeneration is provided by the changing level of free radioactive leucine, which shows a marked turning point at 24 h after fragmentation. Analysis of free -amino concentrations in small regions of larger fragments also indicates the establishment of new intercellular correlations only a few hours after isolation of the cells from the meristem. Up to the 8th h after fragmentation, the contents of both the adaxial and peripheral fragment regions increase. Thereafter only the adaxial (regenerating) cells continue accumulating; the peripheral (nonregenerating) ones remain at the same level.     

Ecology of phlebotomine sand flies in the rural community of Mont Rolland (Thiès region, Senegal): area of transmission of canine leishmaniasis

Background

Different epidemiological studies previously indicated that canine leishmaniasis is present in the region of Thiès (Senegal). However, the risks to human health, the transmission cycle and particularly the implicated vectors are unknown.

Methodology/Principal Findings

To improve our knowledge on the population of phlebotomine sand flies and the potential vectors of canine leishmaniasis, sand flies were collected using sticky traps, light traps and indoor spraying method using pyrethroid insecticides in 16 villages of the rural community of Mont Rolland (Thiès region) between March and July 2005. The 3788 phlebotomine sand flies we collected (2044 males, 1744 females) were distributed among 9 species of which 2 belonged to the genus Phlebotomus: P. duboscqi (vector of cutaneous leishmaniasis in Senegal) and P. rodhaini. The other species belonged to the genus Sergentomyia: S. adleri, S. clydei, S. antennata, S. buxtoni, S. dubia, S. schwetzi and S. magna. The number of individuals and the species composition differed according to the type of trap, suggesting variable, species-related degrees of endophily or exophily. The two species of the genus Phlebotomus were markedly under-represented in comparison to the species of the genus Sergentomyia. This study also shows a heterogeneous spatial distribution within the rural community that could be explained by the different ecosystems and particularly the soil characteristics of this community. Finally, the presence of the S. dubia species appeared to be significantly associated with canine leishmaniasis seroprevalence in dogs.

Conclusions/Significance

Our data allow us to hypothesize that the species of the genus Sergentomyia and particularly the species S. dubia and S. schwetzi might be capable of transmitting canine leishmaniasis. These results challenge the dogma that leishmaniasis is exclusively transmitted by species of the genus Phlebotomus in the Old World. This hypothesis should be more thoroughly evaluated.     

Structural analysis of carrageenans from the red alga, Callophyllis hombroniana Mont. Kütz (Kallymeniaceae, Rhodophyta)

The use of a range of modern analytical techniques has facilitated the structural characterisation of the polysaccharide from the New Zealand endemic red alga, Callophyllis hombroniana. The native polysaccharide contains a number of structural units with the largest proportion consisting of 3-linked beta-D-galactopyranosyl 2-sulfate units, alternating with 4-linked 3,6-anhydro-alpha-D-galactopyranosyl 2-sulfate units, that is, theta-carrageenan (36 mol%). C. hombroniana is the first red seaweed reported to naturally contain such a large proportion of theta-carrageenan.     

Der Einfluß des Antiauxins p-Chlorophenoxyisobuttersäure auf die Bildung meristematischer Zentren bei der Regeneration isolierter Gewebefragmente von Riella helicophylla (Bory et Mont.) Mont.

Summary In isolated tissue fragments of Riella cultured in continuous light, p-Chlorophenoxyisobutyric acid (PCIB) strongly accelerates the polar cell divisions without changing the time of onset of the first nuclear divisions and totally inhibits the formation of unicellular rhizoids. No lateral meristems develop in the cauloids as they do in the control; instead the cells have uniform minimal size. Only preincubating the whole plant in PCIB before the fragments are cut results in a depolarisation with a much higher cell division activity. Preincubation of the whole plant followed by further incubation of the tissue fragments in PCIB decreases the area in which cell embryonization takes place, but the number of cell divisions therein increases to a still larger extent.     

新西兰Ulota germana(Mont.) Mitt.(Orthotrichaceae)的再发现（英文）

Ulota germana(Mont.)Mitt.,之前被作为U.rufula(Mitt.)A.Jaeger,仅在新西兰南岛的马尔堡区有记录,而且该种已经六十余年未被发现过。本文增加了一个新地点:尼尔森区,丹尼斯顿,靠近丹尼斯顿陡坡顶端停车区域。新西兰的Ulota germana与该种的南美居群形态基本一致,除了叶片,雌苞叶和孢子的大小变异。这一新标本被很好地描述,特别是补充了之前研究所缺失的蒴盖和蒴帽特征。本文还提供了Ulota germana的地理分布图以及该种与相近种的形态区别。Ulota pygmaeothecia(Müll.Hal.)Kindb.已被处理为U.luteola(Hook.f.Wilson)WijkMargad.的异名且从新西兰苔藓志中排除。     

Taxonomic and nomenclatural notes on Syrrhopodon gaudichaudii Mont. (Calymperaceae) and S. hawaiicus Müll.Hal.

Syrrhopodon gaudichaudii Mont. is lectotypified and its key features are discussed. A neotype is designated for Syrrhopodon hawaiicus Müll.Hal. and the taxon is placed in synonymy with S. gaudichaudii. A key to the species of Syrrhopodon occurring in the Hawaiian Islands is provided.     

Die Widerstandsfähigkeit von Kartoffelsorten gegenüber der durchPhytophthora infestans (Mont.) De Bary hervorgerufenen Krautfäule

Zusammenfassung In den Jahren 1955 bis 1957 wurden 383 Kulturkartoffelsorten auf ihr Verhalten gegenüber 15 Rassen derPhytophthora infestans geprüft und dabei festgestellt, daß 61 Sorten das Gen R₁ und 7 Sorten andere R-Gene besitzen. Aus den Stammbäumen ist zu entnehmen, daß die R-Gene in allen Fällen ausSolanum demissum bzw. den W-Rassen vonK. O. Müller, die ihrerseits auch aufSolanum demissum zurückgehen, stammen.Von 1950 bis 1957 wurden an dem gleichen Sortiment Beobachtungen über die Feldresistenz gegenüber derPhytophthora infestans durchgeführt. Dabei zeigte sich, daß auch unter den Frühkartoffeln Sorten mit relativ hoher Feldresistenz vorkommen und daß der prozentuale Anteil von Sorten mit hoher Feldresistenz in der Gruppe, die das Gen R₁ enthält, höher ist als in der Gruppe mit r.Herrn Prof.von Sengbusch zum 60. Geburtstag gewidmet.     

Gauchergasilus,a new genus for Ergasilus euripedesi Montú, 1980, an abundant parasitic copepod from the Patos Lagoon in southern Brazil

Ergasilus euripedesi is redescribed from newly collected material of both sexes. A new genus, Gauchergasilus, is proposed, with Gauchergasilus euripedesi n. comb., formerly Ergasilus euripedsi Montú, 1980, as its type and only species. The new genus is characterised principally by the five-segmented antennule, the presence of a conspicuous barb on the concave margin of the claw of the antenna, the two-segmented endopod of leg 1 and the reduced fifth leg comprising two setae, one papillate. The male lacks the barb on the antennal claw but its other appendages are similar to those of the female, except for the maxilliped which is absent in females. The affinities of the new genus lie with the Acusicola group of genera.     

Les Bryozoaires de la formation d'âge paléocène (Danien probable)du Mont Aimé (Marne,Bassin parisien)

The Mont Aimé Formation, of doubtful age, likely paleocene and probably danian, yielded an association of 7 bryozoan species. Two of them are new: Plagioecia bibensis and Beisselina bigeyae. The stratigraphic conclusions are reduced because the other occuring species, among which are of great importance Planicellaria sp. aff. oculata d'Orbigny and Nellia sp. aff. stricta Labracherie, are probably new. The Bryozoans, which lived in an environment with strong terrigenous discharges, resisted to the mud invasion by means of the high number of their avicularia or respectively of the flexibility of their jointed zoaria or of their substratum.     

Syrrhopodon cuneifolius Thér. (Calymperaceae), a good species endemic to Madagascar,with notes on S. leprieurii Mont. from the American tropics

Syrrhopodon cuneifolius Thér. is lectotypified and confirmed as a good species, endemic to Madagascar. The features distinguishing it from the superficially similar Syrrhopodon leprieurii Mont., and morphological variation in the latter are discussed. In contrast to the leaves in S. leprieurii, those in S. cuneifolius generally lack large spines, possess a costa with a ventral cortex of small pluripapillose cells, and have a marginal stereome that fails short of the leaf apex. Consideration is given to removing Syrrhopodon recurvulus Mitt. from synonymy with S. leprieurii and placing it in synonymy with Syrrhopodon gaudichaudii Mont.     

A comparative analysis of karyotypes of three species of Macleaya—producers of a complex of isoquinoline alkaloids

Using a set of methods (C-banding, DAPI-staining, fluorescence hybridization in situ (FISH) with probes of 26S and 5S rDNA, and analysis of meiosis), the first comparative cytogenetic study of three species of Macleaya, producers of complex isoquinoline alkaloids, cordate Macleaya cordata (Willd.) R. Br. (2n = 20), small-fruited Macleaya microcarpa (Maxim.) Fedde (2n = 20) and Macleaya kewensis Turrill (2n = 20), was first carried out. On the basis of morphometric analysis, formulas of karyotypes were made for each species. Species ideograms for M. cordata, M. microcarpa, and M. kewensis were constructed taking into account the polymorphic variants of the C-banding patterns and indicating the location of 26S and 5S rDNA sites. A comparative study revealed that the karyotypes of M. microcarpa and M. kewensis have more in common with each other than with M. cordata. Analysis of meiotic chromosomes suggests of genetic stability of Macleaya genomes. The results of chromosome analysis were used to confirm the close relationship of Macleaya and to clarify their phylogenetic relationships.     

Effect of γ-Irradiation on Development of Lachrymator of Onion

A thermosensitive uracil requiring mutant of Bacillus subtilis Marburg 168 thy trp₂ ts42 was examined as to the colony forming ability at the permissive and nonpermissive temperatures. The viability of the mutant cells decreased rapidly at the restrictive temperature in the modified Woese’s (MW) medium. However, the cells retained viability when sodium succinate or potassium chloride was added to the medium at that temperature although uracil deficiency was unchanged. A little but significant incorporation of adenine-8-¹⁴C into RNA still continued even after the incorporation of N-acetyl-³H-d-glucosamine into acid insoluble fraction of the cells terminated in the MW medium at 48°C. Both incorporations as well as increase of absorbance were slowed down in the presence of sodium succinate at 48°C. This mutant, ts42, was more sensitive to deoxycholate (DOC) than the parent strain. The restoration of colony forming ability after the temperature shift back from 48 to 37°C was suppressed by the addition of DOC to the medium. However, the cell became resistant to DOC when uracil was added to the medium prior to the temperature shift.     

Mechanisms of action of the α-amylase of Micromonospora melanosporea

The -amylase of Micromonospora melanosporea was produced extracellularly during batch fermentation in a 5.0-1 fermentor. The absence of an organic nitrogen source in its growth medium facilitated subsequent purification of the enzyme by ammonium sulphate fractionation and two consecutive Superose-12 gel-filtration steps. The enzyme exhibited maxima for activity at pH 7.0 and 55° C and was 72% stable at pH 6.0–12.0 for 30 min at 40° C. It had a relative molecular mass of 45 000 and an isoelectric point at pH 7.6. The enzyme catalyses the conversion of starch to maltose (53%, w/w) as the predominant final end-product. Initial hydrolysis of this substrate, however, gave rise to the formation of maltooligosaccharides in the range maltotriose to maltohexaose. Maximum yields of these intermediate sugars accumulated to between 31 and 42% (w/w) as the reaction proceeded. The action of the M. melanosporea amylase on high concentrations of saccharides larger than maltotriose resulted in the formation of mainly maltose and maltotriose without concomitant glucose production. A combination of hydrolytic and transfer events is postulated to be responsible for this phenomenon and for the high maltose levels achieved. Correspondence to: C. T. Kelly     

Une nouvelle espèce de Pseudamaurops Jeannel, 1948 du Monténégro,et remarques sur les genres voisins (Coleoptera: Pselaphinae: Amauropini)

Une nouvelle espèce du genre Pseudamaurops Jeannel, 1948, récoltée dans une grotte à proximité de Virpazar, au Monténégro, et décrite et illustrée. Il s’agit là du troisième représentant du genre, deux autres espèces ayant été décrites d’Albanie. La position taxonomique de la nouvelle espèce est discutée, quelques remarques sur le genre et deux genres apparentés, Bergrothia Reitter, 1884 et Zoufalia Reitter, 1918, sont fournies.     

Selection of optimal variants of Gō-like models of proteins through studies of stretching

The Gō-like models of proteins are constructed based on the knowledge of the native conformation. However, there are many possible choices of a Hamiltonian for which the ground state coincides with the native state. Here, we propose to use experimental data on protein stretching to determine what choices are most adequate physically. This criterion is motivated by the fact that stretching processes usually start with the native structure, in the vicinity of which the Gō-like models should work the best. Our selection procedure is applied to 62 different versions of the Gō model and is based on 28 proteins. We consider different potentials, contact maps, local stiffness energies, and energy scales—uniform and nonuniform. In the latter case, the strength of the nonuniformity was governed either by specificity or by properties related to positioning of the side groups. Among them is the simplest variant: uniform couplings with no i, i + 2 contacts. This choice also leads to good folding properties in most cases. We elucidate relationship between the local stiffness described by a potential which involves local chirality and the one which involves dihedral and bond angles. The latter stiffness improves folding but there is little difference between them when it comes to stretching.     

Mechanism of action of α-galactosidase

The effect ofpH on K_m and V_max values of coconut α-galactosidase indicates the involvement of two ionizing groups with pK_a values of 3.5 and 6.5 in catalysis. Chemical modification has indicated the presence of two carboxyl groups, a tryptophan and a tyrosine, at or near the active site of α-galactosidase. Based on these facts a new mechanism of action for α-galactosidase is proposed in which the ionizing group with a pK_a of 3.5 is a carboxyl group involved in stabilizing a carbonium ion intermediate and the ionizing group with a pK_a of 6.5 is a carboxyl group perturbed due to the presence of a hydrophobic residues in its vicinity which donates a H⁺ ion in catalysis.     

Chemistry of the “molecular trap” of protease-catalyzed splicing reaction of complementary segments of α-subunit of hemoglobin A

The complementary fragments of human Hb α, α_1–30, and α_31–141 are spliced together by V8 protease in the presence of 30%n-propanol to generate the full-length molecule (Hb α-semisynthetic reaction). Unlike the other protease-catalyzed protein/peptide splicing reactions of fragment complementing systems, the enzymic condensation of nonassociating segments of Hb α is facilitated by the organic cosolvent induced α-helical conformation of product acting as the “molecular trap” of the splicing reaction. The segments α_24–30 and α_31–40 are the shortest complementary segments that can be spliced by V8 protease. In the present study, the chemistry of the contiguous segment (product) α_24–40 has been manipulated by engineering the amino acid replacements to the positions α²⁷ and α³¹ to delineate the structural basis of the molecular trap. The location of Glu²⁷ and Arg³¹ residues in the contiguous segment α_24–40 (as well as in other larger segments) is ideal to generate (i, i+4) side-chain carboxylate-guanidino interaction in its α-helical conformation. The amino acid residue replacement studies have confirmed that the side chains at α²⁷ and α³¹ facilitate the semisynthetic reaction. The relative influence of the substitute at these sites on the splicing reaction depends on the chemical nature of the side chain and the location. The γ-carboxylate guanidino side-chain interaction appears to contribute up to a maximum of 85% of the thermodynamic stability of the molecular trap. The studies also demonstrate that the thermodynamic stability of the molecular trap is determined by two interdependent conformational aspects of the peptide. One is an amino acid-sequence-specific event that facilitates the induction of an α-helical conformation to the contiguous segment in the presence of organic cosolvent that imparts some amount of protease resistance to Glu³⁰-Arg³¹ peptide bond. The second structural aspect is a site-specific event, ani, i+4 side-chain interaction in the α-helical conformation of the peptide which imparts an additional thermodynamic stability to the molecular trap. The results suggest that conformationally driven “molecular traps” of protease-mediated ligation reactions of peptides could be designed into products to facilitate the modular assembly of peptides/proteins.     

Association of the subunits of the calcium-independent receptor of α-latrotoxin

CIRL-1 also called latrophilin 1 or CL belongs to the family of adhesion G protein-coupled receptors (GPCRs). As all members of adhesion GPSR family CIRL-1 consists of two heterologous subunits, extracellular hydrophilic p120 and heptahelical membrane protein p85. Both CIRL-1 subunits are encoded by one gene but as a result of intracellular proteolysis of precursor, mature receptor has two-subunit structure. It was also shown that a minor portion of the CIRL-1 receptor complexes dissociates, producing the soluble receptor ectodomain, and this dissociation is due to the second cleavage at the site between the site of primary proteolysis and the first transmembrane domain. Recently model of independent localization p120 and p85 on the cell surface was proposed. In this article we evaluated the amount of p120-p85 complex still presented on the cellular membrane and confirmed that on cell surface major amount of mature CIRL-1 presented as a p120-p85 subunit complex.