磁处理党参药液对小鼠肠推进运动影响的药效研究

目的 :观察磁处理党参药液对小鼠肠推进运动的影响。方法 :实验分为非磁处理党参药液对照组 ,生理盐水对照组 ,0 .1T磁处理试验组和 0 .2 5T磁处理药液试验组 ,采用灌胃给药的途径进行实验观察。结果 :0 .1T和 0 .2 5T磁处理药液组和生理盐水对照组相比较 ,试验组具有显著促进小鼠肠的炭末推进作用 (p <0 .0 1 ) ;非磁处理与磁处理党参药液组相比较 ,0 .1T和 0 .2 5T磁处理药液组对小肠的炭末推进率有着显著的抑制作用 (p <0 .0 1 )。结论 :党参磁处理药液对小鼠肠的推进有明显作用。     

磁场作用对小鼠抗应激能力的影响

目的 :探讨磁场作用对小鼠抗应激能力的影响。方法 :对磁场处理 30分钟和 1 5分钟的两组实验组与非磁场处理的正常对照组进行游泳耐疲劳运动时间的比较。结果 :游泳耐疲劳运动实验表明 30分钟磁场处理组与正常对照组比较 ,动物游泳耐疲劳运动时间延长 ,且具有显著性差异 (p <0 .0 5 ) ;1 5分钟磁场处理组与正常对照组比较 ,动物游泳耐疲劳运动时间无明显差异 (p >0 .0 5 ) ;30分钟磁场处理组与 1 5分钟磁场处理组比较 ,动物游泳耐疲劳运动时间延长 ,且具有显著性差异 (p <0 .0 5 )。结论 :在一段时间( 30天 )内 ,每天给予小鼠 30分钟的磁场处理明显提高了小鼠的抗应激能力 ,而磁场处理 1 5分钟对小鼠的抗应激能力不产生影响。     

牛磺酸对蟾蜍离体心脏活动及蝌蚪抗缺氧能力的影响

研究牛磺酸对蟾蜍离体心脏活动及蝌蚪抗缺氧能力的影响。结果表明 :在气温为 (2 0± 1)℃时 ,浓度为 5g·L- 1 ,10g·L- 1 的牛磺酸任氏液使离体心脏跳动时间明显延长 (p <0 .0 5 ) ,浓度为 1g·L- 1 、5g·L- 1 的牛磺酸水溶液对蝌蚪抗缺氧能力有极显著 (p <0 .0 1)、显著 (p <0 .0 5 )改善     

磁处理党参药液对小白鼠碳粒廓清功能影响的研究

目的 :观察小白鼠饮用磁处理党参药液后对碳粒廓清功能的影响。方法 :用碳粒廓清法比较正常对照组、非磁处理党参药液组与磁处理党参药液组对小白鼠碳粒廓清功能的影响。结果 :与正常对照组比较 ,磁处理党参药液组光密度 (OD)降低 ,差异极显著 (p <0 .0 1 ) ,廓清指数 (K)都有提高 ,差异显著 (p <0 .0 5 ) ,吞噬指数 (α)也有提高 ,其中 0 .2 5T药液组达差异显著 (p <0 .0 5 ) ;与非磁处理药液组比较 ,光密度(OD)逐渐下降 ,当取血时间为 1 0min时 ,0 .2 5T药液组达差异显著 (p <0 .0 5 ) ,廓清指数 (K) ,0 .2 5T药液组有提高 ,但差异不显著 ,吞噬指数 (α)都有提高 ,仅 0 .2 5T药液组达差异显著 (p <0 .0 5 )。结论 :磁处理党参药液具有促进单核巨噬细胞系统吞噬功能的作用 ,对免疫有良好作用。     

磁场作用对小鼠学习记忆的影响

目的 :探讨磁场作用对小鼠学习记忆能力的影响。方法 :应用水迷宫学习模型测定并对比 30分钟磁场处理组、1 5分钟磁场处理组和非磁场处理的正常对照组动物的空间学习记忆能力。结果 :水迷宫学习训练的实验表明 30分钟磁场处理组与正常对照组比较 ,动物到达水下平台的时间延长 ;游程增加 ;平均游速减慢 ,且均具有显著性差异 (p <0 .0 5)。 1 5分钟磁场处理组与正常对照组比较 ,动物到达水下平台的时间延长 ,且具有显著性差异 (p <0 .0 5) ;游程和平均速度与正常对照组相比无显著性差异 (p >0 .0 5)。结论 :磁场处理 30分钟或 1 5分钟损伤小鼠的空间学习记忆能力 ,且以 30分钟的磁场处理作用较强     

二氧化硫吸入对小鼠脑组织的氧化损伤

对昆明小鼠进行不同浓度SO2 吸入试验 ,然后测定脑组织中还原型谷胱甘肽 (GSH)含量、谷胱甘肽过氧化物酶 (GSH Px)活性、超氧化物歧化酶 (SOD)活性及脂质过氧化产物丙二醛 (MDA)含量 ,研究SO2 对小鼠中枢神经系统的氧化损伤作用 .雄鼠脑组织匀浆上清液GSH含量在SO2 浓度为 14mg m3 时明显上升 (P <0 0 5 ) ,浓度为 2 8、5 6和 84mg m3 时极显著降低 (P <0 0 1) ,而雌鼠在14和 2 8mg m3 时无明显变化 (P >0 0 5 ) ,在 5 6和 84mg m3 时极显著降低 (P <0 0 1) .雌雄小鼠的GSH Px活性在 14和 2 8mg m3 时无明显变化 (P >0 0 5 ) ,在 5 6、84mg m3 时均极显著降低 (P <0 0 1,P <0 0 0 1) .在SO2 上述 4种吸入浓度下 ,雌雄小鼠的SOD活性均显著降低 (P <0 0 5 )或极显著降低 (P <0 0 1,P <0 0 0 1) ;雄鼠和雌鼠的脂质过氧化产物丙二醛 (MDA)含量均极显著增高 (P <0 0 1,P <0 0 0 1) .结果表明 ,小鼠脑组织对SO2 的氧化损伤作用非常敏感 ,是SO2 的靶器官之一 ,SO2 的污染可能与某些脑疾患有关     

磁处理党参药液对小鼠血液生理功能影响的研究

目的:观察小白鼠饮食磁处理党参药液后对血液功能的影响。方法:用血球计数仪测定白细胞数(WBC)、红细胞数(RBC)、血红蛋白浓度(HGB)及血小板数(PLT) ,采用比较法比较正常对照组、非磁处理党参药液组与磁处理党参药液组对小白鼠血液功能的影响。结果:与正常对照组比较,磁处理党参药液组白细胞数差异不显著(p >0 .0 5 ) ,红细胞数、血红蛋白浓度显著提高,差异极显著(p <0 .0 1) ,血小板数0 .2 5T药液组达差异显著(p <0 .0 5 ) ,0 .1T药液组差异不显著;与非磁处理药液组比较,磁处理党参药液组白细胞数差异不显著(p >0 .0 5 ) ,而红细胞数、血红蛋白浓度及0 .2 5T血小板数均有提高,差异极显著(p <0 .0 1) ,0 .1T药液组血小板数差异不显著(p >0 .0 5 )。结论:磁处理党参药液对血细胞数变化有明显作用。     

穴位埋磁对家兔实验性动脉粥样硬化的影响

目的 :探讨穴位埋磁对家免实验性动脉硬化的影响 ,为磁场防治动脉粥样硬化提供依据。方法 :随机将实验家免分为三组。喂高脂饲料的家兔 ,在其足三里 (双侧 )、内关 (双侧 )及关元穴位埋磁 90天 ,检测其血清胆固醇 (TC)、低密度脂蛋白 (LDL -c)、甘油三酯 (TG)水平。结果 :检测结果均明显低于对照组 (p <0 .0 1 ) ,高密度脂蛋白 (HDL -c)水平显著高于对照组 (p <0 .0 1 )。主动脉内膜脂纹及粥样灶面积亦均显著小于对照组 (p <0 .0 1 )。结论 :穴位埋磁具有减轻和防治动脉硬化的功效。     

三类抗性种子萌发对酸雨胁迫响应

实验采用 p H2 .0、2 .5、3.0、3.5、4 .0、5 .0模拟酸雨处理培养皿中的水稻 (Oryza sativa)、小麦 (Triticum aestivum)、油菜(Brassica chinensis var. oleifera) 3类抗性种子 ,每皿 5 0粒 ,置恒温培养箱内萌发 (2 5℃ ) ,每天更换 1次酸雨 ,处理与对照均 3次重复。定时测定酸雨胁迫强度、胁迫时间对种子发芽率、发芽势、发芽指数、活力指数、异状发芽率 ,吸水值、呼吸速率、贮藏物质运转效率、贮藏物质消耗率、根长抑制指数与芽长抑制指数的影响。结果表明 ,当酸雨胁迫强度 p H=2 .0～ 2 .5时 ,因胁迫强度过高 ,3类抗性种子皆不萌发 ;胁迫强度 p H≥ 3.0时 ,3类抗性种子 5项萌发指标的变幅是水稻 <小麦 <油菜 ;酸雨伤害阈值是水稻 (p H3.0～ 3.5 ) <小麦 (p H3.5～ 4 .0 ) <油菜 (p H4 .0～ 5 .0 ) ;当胁迫强度 (p H)≥ 2 .5时 ,3类抗性种子 6项生理指标的变幅是水稻 <小麦 <油菜 ,其生理反应阈值 (p H≥ 2 .0 ) <萌发反应阈值 (p H≥ 3.0 ) ;3类抗性种子贮藏物质消耗率、贮藏物质运转率 ,根长抑制指数及芽长抑制指数对酸雨胁迫时间的响应 (组间达到差异显著水平 )时间是 :水稻 (7d、7d,3d、3d)≥小麦 (6 d、6 d,3d、3d)≥油菜 (3d、4 d,3d、3d) ;3类抗性种子对酸雨胁迫强度与胁迫时间的耐受性差异 (     

磁处理白术药液对消化功能影响的药效研究

目的 :研究磁处理白术药液对消化功能影响的药效作用。方法 :用碳末法比较生理盐水、磁处理白术药液与非磁处理白术药液对小鼠肠推进运动的影响 ;用对比法 ,比较磁处理白术药液与正常台氏液及与非磁处理白术药液对离体兔小肠平滑肌收缩运动的影响。结果 :小鼠肠碳末推进率 :与生理盐水组比较 ,磁处理白术药液有促进作用 (p <0 .0 1 ) ,磁处理白术药液与非磁处理白术药液相比差异不显著 ;离体兔小肠平滑肌收缩运动 :与正常台氏液相比 ,磁处理白术药液有促进作用 (p <0 .0 1 ) ,磁处理白术药液与非磁处理白术药液相比 ,有抑制作用。结论 :磁处理白术药液对小鼠肠推进运动功能及对离体兔小肠平滑肌收缩运动有明显作用。     

牛磺酸对麦穗鱼抗缺氧能力的影响

在水中溶氧量一定的条件下 ,把麦穗鱼放在不同浓度的牛磺酸水溶液中进行密闭缺氧实验 ,探讨牛磺酸对麦穗鱼抗缺氧能力的影响 ,结果显示 :在 1 4 .5℃～ 1 7.5℃水温下 ,5‰和 1 0‰牛磺酸溶液对麦穗鱼耐缺氧能力有显著增强作用 (p <0 .0 5 )。     

Specific timing of taurine supplementation affects learning ability in mice

The effects of taurine supplementation on visual discrimination in mice were examined. Taurine, 2-aminoethane-sulphonic acid, found in high concentrations in the central nervous system of mammals and in human milk, has been shown to be essential for development. Male mice were divided into four groups according to taurine supplementation periods. 1) Lifelong: taurine (400 mg/kg/day) was dissolved in distilled water and provided as drinking water. In the prenatal period, taurine was given via the mother. After weaning mice were administered taurine in drinking water. 2) Pre-weaning: mice were exposed to taurine prior to weaning, 3) Post-weaning: mice were exposed to taurine after weaning. 4) Control: no supplementation of taurine. It was shown that the Lifelong group required a longer period of time to acquire visual discrimination than the Control group. Conversely, in the Post-weaning group, mice learned the task faster than Controls. Visual discrimination learning time in the Pre-weaning group showed no significant difference compared with that in the Control group. From these results, we suggest that the perinatal to early postnatal period is a “sensitive period” where taurine supplementation can result in retardation of learning in later life. At the same time, taurine supplementation after weaning improved visual discrimination learning. Thus, timing of taurine supplementation affected learning.     

Adenosine receptor agonists affect taurine release from mouse brain stem slices in ischemia

The release of the inhibitory amino acid taurine is markedly enhanced under ischemic conditions in both adult and developing brain stem, together with a pronounced increase in the release of the neuromodulator adenosine. We now studied the effects of adenosine receptor agonists and antagonists on [³H]taurine release in the brain stem in normoxia and ischemia, using a superfusion system. Under standard conditions, the adenosine A₁ receptor agonist N⁶-cyclohexyladenosine (CHA) potentiated basal taurine release in adult mice, which response was blocked by the antagonist 8-cyclopentyl-1,3-dipropylxanthine (DPCPX). CHA and the A_2a receptor agonist 2-p-(2-carboxyethyl)phenylamino-5′-N-ethylcarboxaminoadenosinehydrochloride (CGS 21680) had no effect on the release in developing mice. In ischemia, CHA depressed both basal and K⁺-stimulated taurine release in developing mice in a receptor-mediated manner, blocked by DPCPX. The A_2a receptor agonist CGS 21680 was also inhibitory. Taurine and adenosine may thus not cooperate in developing mice to prevent ischemic neuronal damage. On the other hand, CGS 21680 enhanced taurine release in the adult brain stem in ischemia, both basal and K⁺-stimulated release being affected. These effects were abolished by the antagonist 3,7-dimethyl-1-propargylxanthine (DMPX), indicating a receptor-mediated process. In this case elevated levels of taurine could be beneficial, protecting against hyperexcitation and excitotoxicity.     

Protective effects of taurine against oxidative stress in the heart of MsrA knockout mice

Taurine has been shown to have potent anti‐oxidant properties under various pathophysiological conditions. We reported previously a cellular dysfunction and mitochondrial damage in cardiac myocytes of methionine sulfoxide reductase A (MsrA) gene knockout mice (MsrA^?/?). In the present study, we have explored the protective effects of taurine against oxidative stress in the heart of MsrA^?/? mice with or without taurine treatment. Cardiac cell contractility and Ca²⁺ dynamics were measured using cell‐based assays and in vivo cardiac function was monitored using high‐resolution echocardiography in the tested animals. Our data have shown that MsrA^?/? mice exhibited a progressive cardiac dysfunction with a significant decrease of ejection fraction (EF) and fraction shortening (FS) at age of 8 months compared to the wild type controls at the same age. However, the dysfunction was corrected in MsrA^?/? mice treated with taurine supplement in the diet for 5 months. We further investigated the cellular mechanism underlying the protective effect of taurine in the heart. Our data indicated that cardiac myocytes from MsrA^?/? mice treated with taurine exhibited an improved cell contraction and could tolerate oxidative stress better. Furthermore, taurine treatment reduced significantly the protein oxidation levels in mitochondria of MsrA^?/? hearts, suggesting an anti‐oxidant effect of taurine in cardiac mitochondria. Our study demonstrates that long‐term treatment of taurine as a diet supplement is beneficial to a heart that is vulnerable to environmental oxidative stresses. J. Cell. Biochem. 113: 3559–3566, 2012. © 2012 Wiley Periodicals, Inc.     

Comparison of Anti-Hyperglycemic Effect of Inorganic Constituents and Organic in Traditional Chinese Medicine,Jinqi Compound Recipe

An attempt has been made to compare the hypoglycemic effect of the organic constituents and the inorganic constituents in traditional Chinese medicine, Jinqi compound recipe. Alloxan-induced hyperglycemic mice were used in the study. The body weights, blood glucose, HbA1c, insulin secretion, and glycogen synthesis of the mice were analyzed respectively. After the mice were administered (oral) with organic constituents, the blood glucose and the HbA1c of alloxan-induced hyperglycemic mice decreased (p < 0.05, p < 0.01) and the glycogen synthesis of alloxan-induced hyperglycemic mice elevated (p < 0.05). Also, the body weight of the alloxan-induced hyperglycemic mice was increased gradually. However, the same result did not occur in the inorganic constituent-treated groups. It is noted that neither organic constituents nor inorganic constituents could elevate the level of serum insulin in the alloxan-induced hyperglycemic mice significantly. It is implied that the hypoglycemic effect for type 2 diabetes was caused by the organic constituents of Jinqi recipe. The results can be used to set new standards to control the quality of the Jinqi recipe.     

Role of taurine supplementation to prevent exercise-induced oxidative stress in healthy young men

Summary. To evaluate the protective effects of taurine supplementation on exercise-induced oxidative stress and exercise performance, eleven men aged 18–20 years were selected to participate in two identical bicycle ergometer exercises until exhaustion. Single cell gel assay (SCG assay) was used to study DNA damage in white blood cells (WBC). Pre-supplementation of taurine, a significant negative correlation was found between plasma taurine concentration before exercise and plasma thiobaribituric-acid reactive substance (TBARS) 6hr after exercise (r=–0.642, p<0.05). WBC showed a significant increase in DNA strand breakage 6hr and 24hr after exercise. Seven-day taurine supplementation reduced serum TBARS before exercise (p<0.05) and resulted in a significantly reduced DNA migration 24hr after exercise (p<0.01). Significant increases were also found in VO₂max, exercise time to exhaustion and maximal workload in test with taurine supplementation (p<0.05). After supplementation, the change in taurine concentration showed positive correlations with the changes in exercise time to exhaustion and maximal workload. The results suggest that taurine may attenuate exercise-induced DNA damage and enhance the capacity of exercise due to its cellular protective properties.     

The Co-effect of Vanadium and Fermented Mushroom of Coprinus comatus on Glycaemic Metabolism

The effect of fermented mushroom of Coprinus comatus rich in vanadium (CCRV) on glycaemic metabolism was studied in this paper. Alloxan-induced hyperglycemic mice were used in this study. The insulin secretion and glycogen synthesis of the mice were analyzed. At the same time, the gluconeogenesis of the normal mice was also determined. The alloxan-damaged pancreatic beta-cells of the mice were also studied in this paper. After the mice were administered (i.g.) with CCRV, the level of insulin secretion and glycogen synthesis of alloxan-induced hyperglycemic mice elevated (p<0.05, p<0.01) and the gluconeogenesis of the normal mice was inhibited (p<0.01). Also, the alloxan-damaged pancreatic beta-cells of the mice were partly recovered gradually after the mice were administered (i.g.) with CCRV 15 days later. These may account for the causes of CCRV-induced significant decreases of the blood glucose in hyperglycemic mice.     

The Protective and Antidotal Effects of Taurine on Hexavalent Chromium-Induced Oxidative Stress in Mice Liver Tissue

Acute exposure to hexavalent chromium [Cr(VI)] compounds can cause hepatotoxicity. Reactive intermediates and free radicals generated during reduction process may be responsible for Cr(VI) toxicity. In this study, the effects of pretreatment or posttreatment of taurine on Cr(VI)-induced oxidative stress and chromium accumulation in liver tissue of Swiss Albino mice were investigated. Single intraperitoneal (ip) potassium dichromate treatment (20 mgCr/kg), as Cr(VI) compound, significantly elevated the level of lipid peroxidation as compared with control group (p < 0.05). This was accompanied by significant decreases in nonprotein sulfhydryls (NPSHs) level, superoxide dismutase (SOD), and catalase (CAT) enzyme activities as well as a significant chromium accumulation in the tissue (p < 0.05). Taurine administration (1 g/kg, ip) before or after Cr(VI) exposure resulted in reduction of lipid peroxidation (p < 0.05) showed rebalancing effect on tissue NPSH levels either in pretreatment or in posttreatment (p < 0.05). Enzyme activities of SOD and CAT were restored by taurine pretreatment (p < 0.05), whereas posttreatment had less pronounced effects on these parameters. On the other hand, taurine treatment, before or after exposure, could exert only slight decreases in tissue Cr levels (p > 0.05). In view of the results, taurine seems to exert some beneficial effects against Cr(VI)-induced oxidative stress in liver tissue.     

Comparison of Hypoglycemic Activity of Trace Elements Absorbed in Fermented Mushroom of <Emphasis Type="Italic">Coprinus comatus</Emphasis>

The effect of fermented mushroom of Coprinus comatus rich in trace elements, including vanadium, chromium, zinc, magnesium, copper, iron, and nickel, on glycemic metabolism was studied in this paper. Alloxan-induced hyperglycemic mice were used in the study. The blood glucose, glycohemoglobin, and glycogen synthesis of the mice were analyzed, respectively. At the same time, the gluconeogenesis of the normal mice was also determined. After the mice were administered (ig) with C. comatus rich in vanadium (CCRV), the blood glucose and the glycohemoglobin of alloxan-induced hyperglycemic mice decreased (p < 0.05, p < 0.01), glycogen synthesis of alloxan-induced hyperglycemic mice elevated (p < 0.01), the gluconeogenesis of the normal mice was inhibited (p < 0.01), and the sugar tolerance of the normal mice was improved. However, the same result did not occur in other groups. Vanadium at lower doses in combination with C. comatus induced significant effect on glycemic metabolism in mice.