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Abstract. The effects of age, sex, mating status, pre-exposure to pheromone, resource (diet) quality, and starvation on the response of Prostephanus truncatus (Horn) (Coleoptera: Bostrichidae) to its male-produced aggregation pheromone were investigated.Age was an important factor determining the level of response to pheromone: young adults were more responsive than older adults.The suitability of the resource in which P.truncatus is present affected its dispersal tendency, but not its sensitivity to pheromone per se. Sex, mating status, brief prior exposure to pheromone, and starvation did not influence the response of the larger grain borer to pheromone.The orientation behaviour of the beetle is described, and it is proposed that the pheromone primarily functions as a 'suitable resource location and colonization signal'. 相似文献
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D. Scholz C. Borgemeister R. H. Markham H.-M. Poehling 《Entomologia Experimentalis et Applicata》1997,85(3):237-245
The influence of population density and aggregation pheromone were investigated as possible flight initiating factors for Prostephanus truncatus (Horn) (Col.: Bostrichidae). Maize grains (175 g in glass jars) were infested with 20, 50, 150 or 300 beetles, all of which were removed after 2 weeks. Flight initiation of the progeny was observed over a period of 4 weeks, with and without the synthetic aggregation pheromone of P. truncatus. Addition of the synthetic pheromone had no influence on the number of beetles taking off. The number of beetles dispersing increased with growing initial and progeny densities. Dispersal rates (% of total progeny dispersing) rose with declining rate till they reached a maximum at ca. 33%. Of the beetles dispersing, 74% left the cultures between 18.00 and 20.00 hours. Surviving progeny per female decreased with increasing initial density. Sex ratios in the initial cultures and in the F1 were ca. 1:1, whereas a higher proportion of females (67%) was found among the dispersing beetles. 相似文献
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Omondi AB Orantes LC VAN DEN Berg J Masiga D Schulthess F 《Molecular ecology resources》2009,9(4):1236-1239
Teretrius nigrescens is a predator of the larger grain borer (LGB) Prostephanus truncatus, an invasive post-harvest pest in Africa. We describe the isolation and characterization of 24 novel polymorphic microsatellite markers and their testing on a population from Honduras. Alleles per locus ranged between 2 and 12, and observed heterozygosity between 0.037 and 0.646. Six loci deviated significantly from Hardy-Weinberg equilibrium and showed evidence of null alleles. These markers will be useful for studies of the predator's population structure and characterizing populations for control of LGB. 相似文献
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M. Camara C. Borgemeister R. H. Markham H.-M. Poehling 《Journal of Applied Entomology》2003,127(6):360-368
Abstract: Extensive collections of Teretrius nigrescens in Mexico, Honduras, and Benin, were electrophoretically analysed to elucidate the prey spectrum of the predator. Both polyacrylamide gel electrophoresis and cellulose acetate electrophoresis were used. Beetles were sampled with pheromone traps, using the synthetic aggregation pheromone of Prostephanus truncatus , and directly from farmers' maize stores. The proportion of electrophoretically detected prey protein from adult T. nigrescens in pheromone traps was low: of the 1108 specimens analysed, only in 34 cases, prey protein could be clearly identified. More frequently, prey protein was detected in adult T. nigrescens sampled in maize stores in Mexico, Honduras, and Benin, with 87 samples showing distinct prey bands of the total 1214 predators analysed. Of the 241 T. nigrescens larvae sampled in maize stores in Benin, 136 showed distinct bands of prey protein. In all samples, P. truncatus was the most frequently detected prey species. The second most often identified prey species was Sitophilus zeamais . The results are discussed with regard to various methods for prey spectrum analysis and specifically the biology of T. nigrescens . 相似文献
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Rhodes H. Makundi Ntuli N. Swila Robert N. Misangu Shazia W.M. Reuben Maulid Mwatawala Anna Sikira 《Archives Of Phytopathology And Plant Protection》2013,46(14):1346-1355
The dynamics of infestation and losses of stored maize due to the larger grain borer (Prostephanus truncatus Horn) and maize weevils (Sitophilus zeamais Motschulsky) were investigated in Tanzania. In single species populations, significantly higher maize damage (P ≤ 0.05) was caused by P. truncatus (23.3%) compared to S. zeamais (6.4%). Weight losses caused by P. truncatus were higher (12.7%) than for S. zeamais (6.7%) under similar conditions. A higher proportion of P. truncatus in the initial infestation population led to significantly higher (≤0.05) maize damage compared to equal or higher proportions of S. zeamais. Irrespective of the initial number of S. zeamais, population size increased to levels higher than for P. truncatus. Maize losses in unimproved storage structures were >80% compared to <2.5% in improved structures under natural infestation. Inter-specific competition appeared to influence the dynamics of infestation, damage levels and weight losses. The first two months of storage were critical for damaging insect populations to build up. 相似文献
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PETER R. WHITE 《Physiological Entomology》1989,14(3):349-359
ABSTRACT. The effects of insect age, sex, mated state and culture density on the attraction of adult saw-toothed grain beetles, Oryzaephilus surinamensis (L.) (Coleoptera: Silvanidae), to sources of food odour and synthetic aggregation pheromone were investigated. In single-insect behavioural assays, insect age affected the attraction to the food odour, but not to the synthetic aggregation pheromone. Conversely, there were sexual differences in the attraction to the aggregation pheromone, but not to the food odour. The effects of the same factors on the antennal response to food odour and synthetic pheromone were also investigated, using the electroantennogram (EAG) technique. Insect age affected the EAG response to food odour, but not to the synthetic pheromone. There were no sexual differences in the EAG responses to either food odour or synthetic pheromone.
The correlation of factors affecting both behavioural and antennal responses, and the possible role of changes in peripheral olfactory receptor sensitivity in the modulation of behaviour are discussed. 相似文献
The correlation of factors affecting both behavioural and antennal responses, and the possible role of changes in peripheral olfactory receptor sensitivity in the modulation of behaviour are discussed. 相似文献
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Electroantennogram (EAG) studies demonstrated that antennae of both male and female Trichoplusia ni have: (1) receptor-neurones sensitive to female pheromone, (2) a low response threshold, (3) an identical mean-percentage EAG curve over a broad concentration range of pheromone, and (4) a similar absolute recovery interval from adaptation to pheromonal stimulation. These factors suggest that antennae of male and female T. ni have homologous and homogeneous acceptor sites for the female pheromone. Pheromonal stimulation of female antennae elicited EAGs with only 25% of the amplitude of those elicited in males. 相似文献
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HENRy y. FADAMIRO 《Physiological Entomology》1997,22(1):29-36
Abstract. . Adult Prostephanus truncatus (Horn) (Coleoptera: Bostrichidae) steering anemotactically upwind to a pheromone source in a wind tunnel, respond normally to, and alter their ground speed according to the direction and speed of, the movement of ground patterns beneath them. By manipulating this optomotor reaction component of their behaviour, studies were conducted on their flight duration. The results show a great deal of individual variation in flight duration, and the general data on single flights were skewed towards short flights. Pre-reproductive and inter-reproductive male and female beetles flew significantly longer than older beetles that had passed their peak of reproduction. There was no significant difference in the flight duration of male and female beetles at any age. The overall results suggest mat younger P. truncatus are capable of long-distance flights, and support the proposition that flight could be important in the spread of the beetle. 相似文献
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T. Bashir L.A. Birkinshaw D.R. Hall R.J. Hodges 《Entomologia Experimentalis et Applicata》2001,101(3):273-280
The behavioural responses of adult male and female Rhyzopertha dominica (Coleoptera, Bostrichidae) to blends of host volatiles and male-produced aggregation pheromone were observed in a four-arm airflow olfactometer. The odour sources used were five pheromone-releasing males each on a single maize grain (lower maize-volatiles blend), five pheromone-releasing males on 500 g of maize (higher maize-volatiles blend) or the host volatiles emanating from 500g of maize (maize volatiles alone). Multiple-choice tests, in which volatiles from all three odour sources were presented in the exposure chamber at the same time, were used to study odour preferences of the males and females. Both sexes showed strongest attraction to the higher maize volatile blend but there were significant sex differences in response to the odour sources. Males spent significantly more time than females in the zone with only maize volatiles, and females spent significantly more time in the higher maize-volatiles zone. However, when odour sources were offered singly, females gave numerically greater responses than males to all sources although this difference was statistically significant for only the lower maize-volatiles blend. As males are more attracted than females to host odours alone it is suggested that they may be more highly adapted to seek out new hosts while females are more inclined to locate a food source by following the pheromone signals produced by males. However, both sexes responded most strongly to the odour source comprising aggregation pheromone with the higher proportion of maize volatiles. 相似文献
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【目的】测定红火蚁Solenopsis invicta Buren工蚁对其跟踪信息素的触角电位(EAG)及行为反应。【方法】解剖红火蚁工蚁的杜氏腺, 用正己烷溶剂提取其分泌的跟踪信息素进行气相色谱-质谱(GC-MS)分析, 并测定了红火蚁工蚁对杜氏腺提取物、 工蚁提取物和合成的法尼烯混合物的EAG和招募行为反应。【结果】通过与合成的法尼烯混合物的气相色谱(GC)保留时间和质谱图比对, 发现杜氏腺提取物的主要成分并不是Z, E-α-法尼烯。EAG测定结果表明, 红火蚁工蚁对杜氏腺提取物、 工蚁提取物及100 μg的法尼烯混合物均有较强的EAG反应, 其次为10 μg和1 μg的法尼烯混合物。在招募行为测定中, 杜氏腺提取物和工蚁提取物招募作用明显, 而10, 1, 0.1和0.01 μg法尼烯混合物的作用均不显著。【结论】Z, E-α-法尼烯不是红火蚁跟踪信息素的主要成分; 红火蚁工蚁对杜氏腺提取物、 工蚁提取物有较强的EAG反应和明显的招募行为反应。 相似文献
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暗黑鳃金龟对性信息素的触角电生理及行为反应 总被引:2,自引:0,他引:2
为了分离鉴定暗黑鳃金龟的性信息素成分并对其功能进行验证,本研究对比摸索了3种触角的处理方法,并进行同一浓度(30 ng·μL-1)二元混合物、不同配比的触角EAG测试。结果包括: 建立了一种鳃叶状触角的触角电位(EAG)测定方法,即将暗黑鳃金龟Holotrichia parallela触角的各鳃叶用针分离开后进行测定,这种方法测得的触角电位反应值较高。雄虫触角对L-异亮氨酸甲酯和(R)-(-)-芳樟醇为7∶1的二元混合物的反应值最高,和暗黑鳃金龟雌虫性信息素25个腺体提取液的EAG反应相当;对同一配比(7∶1)不同剂量刺激液的EAG测试表明,雄虫对浓度为30 ng·μL-1的二元混合物刺激液的反应值最高。嗅觉反应结果进一步证实,试虫对L-异亮氨酸甲酯和(R)-(-)-芳樟醇7∶1的选择最高,选择系数达93.3%。研究结果为利用性信息素防治暗黑鳃金龟技术的开发提供了基础。 相似文献
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A. BLACKWELL C. DYER A. J. MORDUE LUNTZ L. J. WADHAMS† W. MORDUE 《Physiological Entomology》1994,19(4):251-257
Abstract. Electrophysiological, behavioural and field evidence is presented for a volatile attractant produced by host-seeking, parous female Culicoides impunctatus Goetghebuer. Attraction of host-seeking females to other live parous females or to extracts of parous females was demonstrated in Y-tube bioassays, as well as in field trials using baited sticky traps. Threshold numbers were 100–200 females, both in the laboratory and field. Antennal receptors to this pheromone were revealed by recording electroantennograms (EAGs) in response to odour from live parous females and to the extracts of female midges. The female-produced attractant did not alter field catches of male C. impunctatus. 相似文献
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William Tinzaara Marcel Dicke Arnold Van Huis Joop J. A. Van Loon & Clifford S. Gold 《Entomologia Experimentalis et Applicata》2003,106(3):169-175
Abstract Three different bioassay methods to investigate the orientation behaviour of the banana weevil, Cosmopolites sordidus (Germar) (Coleoptera: Curculionidae), to host plant volatiles and a synthetic pheromone (cosmolure+) were compared. A locomotion compensator was used to separately record walking tracks in response to three odour sources. The data show that C. sordidus uses odour‐conditioned anemotaxis in its orientation to the odour sources tested. Of the two olfactometers tested, a dual port olfactometer using a continuous airflow showed stronger discrimination by C. sordidus to the different odours compared with a double pitfall olfactometer. The results of all three bioassays indicate that C. sordidus responds in an additive way to the combination of fermentation plant volatiles and the synthetic pheromone. 相似文献
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D. Obeng-Ofori P. G. N. Njagi B. Torto A. Hassanali H. Amiani 《Entomologia Experimentalis et Applicata》1994,73(1):85-91
Behavioural responses of nymphs and adults in the gregarious phase of the desert locust,Schistocerca gregaria (Forskal) (Orthoptera: Acrididae) were investigated in a single-chamber bioassay system to a choice of two columns of air,
one permeated with airborne volatiles emanating from either sex of nymphs or adults and the other untreated. There was no
sexual differentiation in the production of or response to nymphal volatiles. Young adults of either sex did not produce a
stimulus with significant activity. Of the older adults, only the males produced the aggregation stimulus to which both sexes
were equally responsive. Charcoal-trapped volatiles from the two sexes of nymphs and adults evoked similar aggregation responses.
Antennae of the older adults showed significantly higher EAG responses than those of fifth instar nymphs to all four volatile
collections, of which volatiles from older adult males were the most stimulatory and evoked the highest EAG amplitudes. 相似文献
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The response of adult flower thrips Frankliniella intonsa (Trybom) (Thysanoptera: Thripidae) to conspecifics is investigated in Y‐tube olfactometer bioassays. The results show that both males and females are attracted to the odours of adult males, which indicates a male‐produced aggregation pheromone in this species. Gas chromatography‐mass spectroscopy analyses of headspace volatiles collected on solid‐phase microextraction fibres show that two major components and six minor components are present in volatiles from males but not in females. Further gas chromatography‐mass spectroscopy analyses reveal that the two major components in head‐space volatiles produced by F. intonsa males are the same compounds that are reported in volatiles of Frankliniella occidentalis males: (R)‐lavandulyl acetate and neryl (S)‐2‐methylbutanoate. However, the quantity of these two compounds in the volatiles differs between the species. 相似文献
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The European corn borer Ostrinia nubilalis (Hübner) (Lepidoptera: Crambidae) is a worldwide pest of maize (Zea mays L.) and other crops. The semiochemicals released by maize plants and structurally‐related compounds can be used by adult female O. nubilalis for host‐plant location and oviposition. Headspace volatile compounds emitted by watered and water‐deprived maize plants are collected and identified by their retention indices and mass spectra. The most abundant compounds from watered plants are limonene, linalool, benzoic acid, indole, β‐caryophyllene and acetophenone, whereas, in water‐deprived plants, limonene, acetophenone, hexanoic acid, benzoic acid and indole are dominant. In addition, (E)‐4,8‐dimethyl‐1,3,7‐nonatriene, 6‐methyl‐5‐hepten‐2‐one, anisole and 1‐carvone are undetected in the water‐deprived plants. Some of the identified compounds show electrophysiological activity (electroantennogram) in the antennae of both sexes, with the responses elicited by tridecane, tetradecane, dodecane, nonanal, decanal and 2‐ethylhexanol on males being particularly noteworthy. In a dual‐choice olfactometer, adult females show a preference for 2‐hexanol, heptanal, methyl salicylate, hexyl acetate, nonanal, methyl dodecanoate, β‐pinene and (E)‐2‐hexenyl acetate over hexane controls. Tetradecane, linalool, methyl hexanoate, methyl nonanoate, (Z)‐3‐hexenyl benzoate, tridecane, 2‐cyclopentylcyclopentanone, 3‐methylbutyl acetate, β‐myrcene and (Z)‐3‐hexenyl butanoate result in fewer females in the test arm compared with the control arm. No single compound displays an activity similar to watered maize plants, supporting the hypothesis that blends of volatiles in specific ratios are more effective than single volatile chemicals. The results of the present study suggest that methyl salicylate, which elicits also one of the highest electrophysiological responses in female antennae, plays a role in host preference by O. nubilalis females. 相似文献
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Abstract The effects of age, time of day and pheromone concentration on the responsiveness of the male brown-banded cockroach, Supella longipalpa (F.), to female volatile sex pheromone were examined. Male response increased with age and peaked 10 days after the imaginal moult. A diel periodicity of responsiveness was observed, with greater responses in the scotophase. The behavioural response was dose-dependent and its diel periodicity was most obvious when intermediate pheromone concentrations (e.g. 0.01 female equivalents) were used. Higher concentrations obscured the periodicity by eliciting greater responses in the photophase, while the lower concentrations did so by decreasing the response in the scotophase. Following entrainment to a LD 12:12 h cycle, the periodicity of response to 0.01 female equivalents of pheromone persisted for at least 54 h under continuous darkness, further demonstrating a true circadian rhythm. 相似文献
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The electrophysiological and behavioural responses of male Sesamia nonagrioides Lefèbvre (Lepidoptera: Noctuidae) to several synthetic blends of its sex pheromone components were investigated. Electroantennogram (EAG) recordings showed the greatest response to a 77:8:10:5 blend of the four compounds found in the female glands: (Z)-11-hexadecen-1-yl acetate, (Z)-11-hexadecen-1-ol, (Z)-11-hexadecenal and dodecyl acetate. A logistic equation was fitted to the dose-response data and differences in relative EAG responses were analysed by deriving five parameters of this equation. Flight responses of males were also evaluated in a wind tunnel where the proposed blend (77:8:10:5) also elicited the best response. Field tests confirmed the laboratory findings as the 77:8:10:5 blend captured more S. nonagrioides males than other pheromone mixtures and showed more selectivity for male S. nonagrioides over other noctuid species, Discestra trifolii Rottenberg and Lacanobia oleracea L., but not for Mythimna unipuncta Haworth, a species with a sex pheromone composition almost identical to that of S. nonagrioides. 相似文献