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1.
Summary Treatment of E. coli B bacteria by the base analogue 2-aminopurine, before infection with T4 phages, induces mutations of the transition type into the virus. Treatment of the phage-bacterium complex, only during the first four minutes of the latent period i.e. at a moment where no phage DNA is synthetized, is also mutagenic. The kinetics of acquisition and loss by treated bacteria of mutagenicity and the action of various metabolic inhibitors show that the base analogue is stored into the bacterial or the phage messenger RNA from which it is reused to be incorporated into the phage DNA.This investigation was supported by the Centre National de la Recherche Scientifique (L. A. No 86 et 136), l'Institut Pasteur, la Délégation Générale à la Recherche Scientifique and la Fondation pour la Recherche Medicale. 相似文献
2.
Numerous organizations participate and cooperate on parasitological research in France including the Institut national de la Santé et de la Recherche Médicale (INSERM), the Centre national de la Recherche Scientifique (CNRS), the Institut Pasteur, the Institut Fran?ais de Recherche Scientifique pour le Développement en Coopération (ORSTOMM), the Institut national de la Recherche Agronomique (INRA), the Muséum national d'Histoire naturelle (MNHN), the Universities, the Collège de France, the Ecole Pratique des Hautes Etudes (EPHE) as well as various commercial firms. Exchanges and collaborations with foreign workers are continuous and essential to the success of research on tropical diseases. Here, in their own words, Odile Bain, Daniel Camus and Jacques Prod'hon highlight some aspects of current parasitological research in France. 相似文献
3.
B. Delhotal-Landes F. Lemonnier M. Couturier J. P. Carreau M. Gautier A. Lemonnier 《In vitro cellular & developmental biology. Plant》1987,23(5):355-360
Summary The comparative metabolic effects of fructose and glucose were determined in human fibroblast cultures. Cells were grown in
four different media containing 5.5 and 27.5 mM of glucose and fructose, respectively. For these two hexoses, we compared their uptake, consumption, and conversion into14CO2 and14C-lipids.
D-Fructose was taken up in fibroblasts by an unsaturable process and its consumption was much smaller than that ofD-glucose. Whatever the experimental procedure, the glycogen content of cells grown in fructose media was significantly lower
than of those grown in glucose media. Labeling of fructose and glucose with14C showed that more carbon from fructose than from glucose was incorporated into CO2 and glycerolipids. The relative distribution of14C in the different lipid fractions was similar for both hexoses.
These results indicated that the pathways of intermediary metabolism in fibroblast cultures were influenced by the nature
of the carbohydrate present in the culture medium and that fructose was a better lipogenic substrate than glucose in human
fibroblast cultures.
This work was supported by grants for the Institut National de la Santé et de la Recherche Medicale (ATP 82-79-114). 相似文献
4.
Danchin A 《Bioinformatics (Oxford, England)》2000,16(1):65-75
The development of in silico genomics has progressed slowly in France for a number of political reasons. Two administrative organizations, the Groupement de Recherche sur les Génomes (GREG) and the Groupement de Recherche 1029 (GDR 1029) of the Centre National de la Recherche Scientifique (CNRS) have been established. These organizations have created the dynamics that hopefully will place France (which coordinated consortia that completed several of the first large microbial genomes) among the developed nations that support Large-Scale Biology. 相似文献
5.
Laminin provides a better substrate than fibronectin for attachment,growth, and differentiation of 1003 embryonal carcinoma cells 总被引:3,自引:0,他引:3
Michel Y. Darmon 《In vitro cellular & developmental biology. Plant》1982,18(12):997-1003
Summary Culture of cells in hormonally defined media has allowed (a) the demonstration of physiological responses from cells usually
unable to express them in vitro and (b) the study of the effects on growth and differentiation of diffusible factors and attachment
factors. The embryonal carcinoma line 1003 forms multidifferentiated tumors in vivo but is unable to differentiate in vitro
when grown in serum-containing medium. In a defined medium containing insulin, transferrin, selenium, and fibronectin as attachment
factors, 1003 cells grow for several generations and differentiate into neurons and embryonic mesenchyme (Darmon et al., 1981,
Dev. Biol. 85: 463–473). In the present work the effects of fibronectin and laminin were compared. In the presence of laminin
the cells attached and spread better, grew faster, and could be plated at lower densities. Neurite extension was also better
under these conditions and most importantly, it was found that laminin induced an important formation of muscular tissue when
the cells had been seeded at low densities. Multinucleated myotubes could be stained with antibodies directed against embryonic
muscular myosin. Coating the dishes with polylysine or adding FGF or serum-spreading factor to the medium allowed growth of
low-density cultures with fibronectin instead of laminin but muscular differentiation was not detected under these conditions.
Addition of fibronectin to laminin-containing medium did not inhibit muscular differentiation.
Presented in the symposium on Plant and Animal Physiology in Vitro at the 33rd Annual Meeting of the Tissue Culture Association,
San Diego, California, June 6–10, 1982.
This research was supported in part by grants from the “Centre National de la Recherche Scientifique” (LA 269), the “Délégation
Générale à la Recherche Scientifique et Technique,” the Fondation pour la Recherche Médicale Fran?aise,” the “Institut National
de la Santé et de la Recherche Medicale,” the “Ligue Nationale Fran?aise centre le Cancer,” and the “Fondation André Meyer.”
This symposium was supported in part by the following organizations: Bellco Glass, Inc., California Branch of the Tissue Culture
Association, Collaborative Research, Hana Media, Hybridtech, K C Biological, Inc., and Millipore Corporation. 相似文献
6.
Chantal M. Godard Yvette L. Augery Michèle Ginsbourg Claude Jasmin 《In vitro cellular & developmental biology. Plant》1983,19(12):897-902
Summary Two continuous cell lines derived from long-term cultures of AKR mouse bone marrow adherent cells were isolated. These cell
lines release colony stimulating activity (CSA), a factor that induces in vitro differentiation of granulocyte-macrophage
progenitor cells. The colony forming cells and cluster forming cells in mouse marrow responsive to CSA from cell line conditioned
medium were compared with those responsive to CSA from mouse lung conditioned medium (MLCM). Colony forming cells were characterized
by analysis of their density distribution after equilibrium centrifugation in density gradient. Cluster forming cells were
characterized by analyzing the progeny of individual clusters after transfer to fresh semisolid culture medium containing
MLCM. The results obtained indicate that the CSA from cell line conditioned medium closely compares with the CSA from MLCM
in terms of the populations of colony and cluster forming cells stimulated.
This work was supported by a research grant from the Institut National de la Santé et de la Recherche Médicale (CRL 802620),
Paris, France. 相似文献
7.
Regulation of vascular development by fibroblast growth factors 总被引:5,自引:0,他引:5
Fibroblast growth factors (FGFs) are potent stimulators of angiogenesis in vitro and in vivo. However, the precise role of FGFs and vascular development in normal and pathological tissue has long remained ill defined. Recently, substantial progress has been made toward a better understanding of their role. Genetic studies in mice or in culture systems indicate a role for FGFs in vessel assembly and sprouting. FGFs also stimulate blood vessel branching and lymphangiogenesis. The molecular mechanisms by which FGFs mediate angiogenesis are also better understood. Finally, the FGF/FGF-receptor system has become a focus for the development of novel therapeutic strategies for the treatment of angiogenesis-related diseases such as tissue ischemia.Work described herein from our laboratory was supported by grants from the Ligue Nationale contre le Cancer, the Association de la Recherche sur le Cancer, Rétina France, the Institut National de la Santé et de la Recherche Médicale (INSERM), and the Ministère de la Recherche 相似文献
8.
Christiane Guguen-Guillouzo Marie-France Szanjnert Denise Glaiser Claudine Gregori Fanny Schapira 《In vitro cellular & developmental biology. Plant》1981,17(5):369-377
Summary Aldolase and pyruvate kinase isozymes were investigated in cultured hepatocytes from fetal, regenerating, and 2-acetyl-aminofluorene-fed
rat liver as well as in some epithelial liver cell lines. Our results show that: (a) cell proliferation and prolonged expression
of specific isozymes were found only in cultured hepatocytes from 17-day old fetuses; (b) the fetal type of pyruvate kinase
expressed in regenerating and carcinogen-treated liver was temporarily lost only in cultured hepatocytes from regenerating
liver; (c) the adult type of aldolase and pyruvate kinase was absent in one epithelial cell line derived from a carcinogen-treated
liver and in the hepatoma tissue cell (HTC) line but was found in the Faza clone of the Reuber H35 cell line during the 50 first passages in vitro; and (d) the isozyme pattern of pyruvate kinase was always more strongly
shifted than that of aldolase.
The observations suggest that: (a) hepatocytes from carcinogen-treated liver exhibit the same lack of ability to proliferate
in primary culture as normal adult hepatocytes; (b) adult hepatocytes can produce fetal isozymes without prior cell division;
(c) pyruvate kinase is a stronger marker of dedifferentiation (retrodifferentiation) than aldolase; and (d) regulatory processes
of isozyme expression are different during ontogenesis, regeneration, and hepatocarcinogenesis.
This work was supported by the “Institut National de la Santé et de la Recherche Médicale” and the “Fondation pour la Recherche
Medicale Fran?aise” 相似文献
9.
P. M. Simon M. Kedinger F. Raul J. F. Grenier K. Haffen 《In vitro cellular & developmental biology. Plant》1982,18(4):339-346
Summary Jejunal mucosa of 6 d-old rats were cultured for 24 and 48 h in the presence of thyroxine, insulin, pentagastrin, glucagon,
epidermal growth factor (EGF) or dibutyryl-A-3:5-MP cyclic with or without dexamethasone (DX). The enzymes were assayed on
the purified brush borders. The various agents added alone to the basic culture medium had no effect with the exception of
DX on the levels of enzyme activities. Dexamethasone alone induced sucrase, stimulated maltase, and protected other brush
border enzyme activities (aminopeptidase, lactase, and alkaline phosphatase). When added to DX-supplemented medium, only the
following factors modified the levels of enzymatic activities observed with DX alone. Insulin (10−6
M) increased maltase, alkaline phosphatase, and lactase activity to a greater extent than DX at 24 h culture, the effect being
maintained at 48 h on alkaline phosphatase only. At 48 h culture, both EGF (10−8
M) and dbcAMP (10−3
M) decreased DX-induced sucrase activity. The latter agent also depressed DX-stimulated aminopeptidase activity.
This work was supported by the Institut National de la Santé et de la Recherche Médicale, Centre National de la Recherche
Scientifique, and a grant 79.7.1243 from the Délégation Générale a la Recherche Scientifique et Technique.
P. M. S. is a recipient of a grant from Fondation de la Recherche Médicale (France). 相似文献
10.
F. Pelloquin J. P. Lamelin G. M. Lenoir 《In vitro cellular & developmental biology. Plant》1986,22(12):689-694
Summary A simple method was devised for the establishment of continuous lymphoblastoid, cell lines (LCL). Unfractionated mononuclear
cells collected from healthy donors were infected in vitro by Epstein-Barr Virus (EBV) (strain B95-8) under specific, conditions:
an immunosuppressive drug, Cyclosporin-A (CS-A, Sandoz), associated with the use of a feeder layer (MRC-5) led to 100% efficiency
of LCL establishment. A bank of 400 LCL was set up for completion of genetic studies. Regression and kinetics of virus-induced
transformation were monitored and related to donors' EBV immune status. Mean time of LCL establishment and probability of
regression among seropositive donors were not linked to any given value titer of antibodies against Viral Capsid Antigen (VCA)
or against Epstein-Barr Nuclear Antigen (EBNA). However, when the anti-VCA:anti-EBNA ratio was considered, this parameter,
seemed to be linked to the kinetics of transformation but not to the probability of regression. Once LCL are established,
large quantities of human cells can be produced. The complete cellular DNA is available so that any part of it can be scrutinized.
Moreover, some of the phenotypic characteristics of these B cells be used for a wide range of investigations.
The work was supported by Fondation de la Recherche Medicale (France) for Professor J. Dausset (Paris) and by the International
Agency for Research on Cancer (Lyon). 相似文献
11.
Genetic diversity and molecular detection of three toxic dinoflagellate genera (Alexandrium,Dinophysis, and Karenia) from French coasts 总被引:6,自引:0,他引:6
Guillou L Nézan E Cueff V Erard-Le Denn E Cambon-Bonavita MA Gentien P Barbier G 《Protist》2002,153(3):223-238
The objectives of this study were 1) to study the genetic diversity of the Alexandrium, Dinophysis and Karenia genera along the French coasts in order to design probes targeting specific DNA regions, and 2) to apply PCR-based detection to detect these three toxic dinoflagellate genera in natural samples. Genetic diversity of these toxic taxa was first studied from either cultures or cells isolated from Lugol-fixed field samples. By this way, partial sequences of the large ribosomal subunit (LSU rDNA) including the variable domains D1 and D2 of A. minutum, Alexandrium species inside the tamarensis complex, the D. acuminata complex and K. mikimotoi were obtained. Next, specific primers were designed for a selection of toxic algae and used during semi-nested PCR detection. This method was tested over a 3-month period on water samples from the Bay of Concarneau (Brittany, France) and on sediment from the Antifer harbor (The English Channel, France). Specificity and sensitivity of this molecular detection were evaluated using the occurrence of target taxa reported by the IFREMER (Institut Fran?ais de Recherche pour l'Exploitation de la Mer) monitoring network based on conventional microscopic examination. This work presents the first results obtained on the biogeographical distribution of genotypes of these three toxic genera along the French coasts. 相似文献
12.
Roeland van Wijk Lydie Tichonicky Jaques Kruh 《In vitro cellular & developmental biology. Plant》1981,17(10):859-862
Summary Exposure of HTC cells to sodium butyrate caused inhibition of growth. The site of growth inhibition was studied by time-lapse
cinematography and [3H]thymidine incorporation studies. Evidence is presented that sodiunm butyrate affected the cell cycle at a specific point
immediately after mitosis. Inasmuch as it does not modify the interphase duration after its removal, butyrate may be used
for HTC synchronization.
This work was supported by l'Institut Nationale de la Santé et de la Recherche Médicale and la Centre Nationale de la Recherche
Scientifique (L. T. and J. K.). 相似文献
13.
Radovan Borojevic Alvaro N. A. Monteiro Solange A. Vinhas Gilberto B. Domont Paulo A. S. Mourão Hervé Emonard Gabriel Grimaldi Jr. Jean-Alexis Grimaud 《In vitro cellular & developmental biology. Plant》1985,21(7):382-390
Summary A continuous murine cell line (GRX) was obtained from fibrotic granulomas induced in C3H/HeN mice liver by experimental infection
withSchistosoma mansoni. This anchorage-dependent line produces composite connective tissue/extracellular matrix, displays morphological characteristics
of myofibroblasts, and can, under appropriate conditions, accumulate fat droplets. GRX cells produce viral particles of retrovirus
type. We consider GRX cell line to be representative of liver connective tissue cells, responsible for fibroplasia in liver
fibrotic and granulomatous reactions.
This research was supported by Centre National de la Recherche Scientifique, France; Financiadora de Estudos e Projetos (FINEP),
Brasil; and Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Brasil. 相似文献
14.
Alain Empain 《Hydrobiologia》1978,60(1):49-74
Résumé L'étude physico-chimique des eaux, couplée à celle de la répartition quantitative des espèces bryophytiques aquatiques et subaquatiques de la Sambre, de la Somme et de la Meuse (Belgique et Nord de la France), permet de tirer quelques conclusions quant à la résistance de ces espèces aux différents types de pollutions.La synthèse multivariable des résultats aboutit à l'élaboration d'un indice de qualité des eaux, qui est comparé avec l'indice similaire de Descy, relatif aux diatomées benthiques.
The physico-chemical analysis of the waters, in conjunction with the quantitative distribution of the aquatic and subaquatic bryophytes in the rivers Sambre, Somme and Meuse (Belgium and North France), has led to conclusions about species resistance to different kinds of pollution.The data's multivariable synthesis gives a quality index of water and permits comparison with Descy's index for benthic diatoms.
Recherches bénéficiant de l'appui du Fonds de la Recherche Fondamentale Collective: programme du Centre de recherches fondamentales sur les bioindicateurs de la pollution des milieux continentaux. 相似文献
15.
Marie-France Grasset Jean Paul Blanchet 《In vitro cellular & developmental biology. Plant》1984,20(4):302-304
Summary The growth of late erythroid precursors (CFU-Es) from adult bone marrow is inhibited when Iscove's modified Dulbecco's medium
supplied in liquid form is used. Catalase and other H2O2 destroying compounds restore the capacity of culture medium to support colony development. However early precursors from
adult bone marrow and fetal liver CFU-Es were resistant to H2O2.
This work was supported by grants from the Centre National de la Recherche Scientifique, the Institut National de la Santé
et de la Recherche Médicale and the Fédération Nationale des Centres de Lutte contre le Cancer. 相似文献
16.
《Epigenetics》2013,8(4):415-418
With the goal of discussing how epigenetic control and chromatin remodeling contribute to the various processes that lead to cellular plasticity and disease, this symposium marks the collaboration between the Institut National de la Santé et de la Recherche Médicale (INSERM) in France and the University of California, Irvine (UCI). Organized by Paolo Sassone-Corsi (UCI) and held at the Beckman Center of the National Academy of Sciences at the UCI campus December 15–16, 2011, this was the first of a series of international conferences on epigenetics dedicated to the scientific community in Southern California. The meeting also served as the official kick off for the newly formed Center for Epigenetics and Metabolism at the School of Medicine, UCI (http://cem.igb.uci.edu). 相似文献
17.
Barbara P. Rattner 《Epigenetics》2012,7(4):415-418
With the goal of discussing how epigenetic control and chromatin remodeling contribute to the various processes that lead to cellular plasticity and disease, this symposium marks the collaboration between the Institut National de la Santé et de la Recherche Médicale (INSERM) in France and the University of California, Irvine (UCI). Organized by Paolo Sassone-Corsi (UCI) and held at the Beckman Center of the National Academy of Sciences at the UCI campus December 15–16, 2011, this was the first of a series of international conferences on epigenetics dedicated to the scientific community in Southern California. The meeting also served as the official kick off for the newly formed Center for Epigenetics and Metabolism at the School of Medicine, UCI (http://cem.igb.uci.edu). 相似文献
18.
D. Gaillard R. Négrel G. Serrero-Davé C. Cermolacce G. Ailhaud 《In vitro cellular & developmental biology. Plant》1984,20(2):79-88
Summary Ob17 is a clonal cell line isolated from the epididymal fat pad of C57 BL/6J ob/ob mouse that differentiates into adiposelike
cells in serum-supplemented medium. In serum-free medium, this cell line shows increased growth under the addition of insulin,
transferrin, fibroblast growth factor (FGF), and a factor present in extract of rat submaxillary gland (SMGE). This medium
is referred to as 4F. Epidermal growth factor or nerve growth factor cannot replace SMGE, whereas partially purified platelet
extract can substitute for FGF but only partially for SMGE. 4F Medium is able to support the proliferation of cells from other
established preadipocyte clonal lines, HGFu and 3T3-F442A, and also of preadipocyte cells isolated from the stromal-vascular
fraction of rat and mouse adipose tissues. In each case 4F medium is insufficient to support the differentiation of these
cells into adipocytes. Ob17 cells grown and maintained in serum-free hormone-supplemented medium retain the ability to convert
to adiposelike cells after serum addition. This serum requirement for differentiation cannot be substituted by the addition
of growth hormone or of other putative adipogenic factors, or both. The results are discussed with respect to the requirements
for growth and differentiation of the 3T3-L1 and 1246 preadipocyte cell lines previously described.
This work was supported by the “Centre National de la Recherche Scientifique” (Grant 1208-Biochimie du Développement and Grant
4162-Endocrinologie), by the “Ministère de la Recherce et de la Technologie” (Grant 81-L-1322), by the “Fondation pour la
Recherche Médicale,” by NATO (Grant 1704), and by the “Institut National de la Santé et de la Recherche Médicale” (Grant 827006). 相似文献
19.
Summary The fine structure of ADH-induced intramembrane particle aggregates has been studied in different tissues and under different experimental conditions. Particle aggregates similar to those previously observed in the amphibian urinary bladder and in the mammalian collecting duct were also found in the frog skin, another ADH target tissue. In the frog urinary bladder, typical aggregates were observed in the absence of glutaraldehyde fixation. Two experimental approaches were used a) the absence of both fixative and cryoprotectant treatments and b) the absence of only glutaraldehyde treatment. In the latter case the reversal of hydrosmotic action was prevented by exposing the preparations to N-ethyl maleimide. In specimens of frog urinary bladder conventionally fixed with glutaraldehyde, two fracture levels could be observed in the aggregates, suggesting that the aggregated particles span an appreciable part of the membrane thickness.J. Chevalier is a career investigator from the Institut National de la Santé et de la Recherche Médicale, INSERM U 48, France 相似文献
20.
Resumé Les grains de protéine sécrétés par la glande mammaire, révélés par le microscope électronique, peuvent s'individualiser au microscope optique à condition d'employer une technique assez fine.
Avec l'aide du Centre National de la Recherche Scientifique et de l'Institut National de la Santé et de la Recherche Médicale. 相似文献
Summary The protein granules secreted by the mammary gland and first revealed by electron microscopy can be seen in the light microscope, if appropriate techniques are emploied.
Avec l'aide du Centre National de la Recherche Scientifique et de l'Institut National de la Santé et de la Recherche Médicale. 相似文献