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1.
Bacillus thuringiensis var israelensis (BTI), when ingested by mosquito larvae, is highly toxic to them. Many other aquatic invertebrates feed on bacteria but, in general, BTI is not toxic to them. We tested in the laboratory the hypothesis that certain crustaceans indirectly benefit mosquito larvae by reducing mortality caused by BTI. We presumed the mechanism to be ingestion of the bacteria by the crustaceans resulting in a lower concentration available to the mosquito larvae.Mortality of Aedes aegypti larvae exposed to BTI was reduced in the presence of the fairy shrimp, Branchipus schaefferi and the ostracod, Cypridopsis vidua (only during summer trials and not autumn trials for the latter species) but was not reduced in the presence of the ostracod, Heterocypris incongruens. By contrast, H. incongruens preyed upon infected, though still-moving, larvae. Feeding on the bacteria by the crustaceans may not be an important mechanism; our data indicates that the crustaceans did not reduce BTI in the water. Moreover, B. schaefferi, introduced into water and then removed prior to the introduction of BTI and mosquitoes, also reduced mosquito mortality.The mechanism for the protective effect of B. schaefferi and C. vidua is unknown. However, these results suggest that the abundance of certain organisms co-occurring with mosquito larvae may partially explain why the effective concentration of BTI varies among habitats. 相似文献
2.
Aedes aegypti from the Brazilian cities of São José do Rio Preto (SJ) and Goiânia (GO) were analyzed as to their esterase patterns and the results were compared with data obtained about 5 years before for SJ population. Esterase bands not detected in the previous study were now observed in mosquitoes from both SJ and GO populations, being the last considered a population resistant to insecticides. Other similarities between SJ and GO populations in this study, and some differences in comparison with the previous data on SJ were observed, involving, in addition to changes in band type, changes in frequency of mosquitoes expressing them and differential gene activation during development. As it is generally true for genetic features, changes in the esterase patterns are expected to be the result of factors such as selection by environmental conditions and genetic drift. In the present case, continuous use of insecticides aiming mosquito population size control in SJ by sanitary authorities could be involved in the observed changes. Changed esterases were classified as carboxylesterases and cholinesterases, which are enzymes already shown to take part in the development of resistance in several organisms. In addition, data obtained in the elapsed time by authorities responsible for the mosquito control has shown increasing insecticide resistance of SJ population mosquitoes parallel to increase in the total amount of esterases, reinforcing the mentioned possibility. 相似文献
3.
Aedes aegypti is the main vector for dengue and urban yellow fever. It is extended around the world not only in the tropical regions but
also beyond them, reaching temperate climates. Because of its importance as a vector of deadly diseases, the significance
of its distribution in urban areas and the possibility of breeding in laboratory facilities, Aedes aegypti is one of the best-known mosquitoes. In this work the biology of Aedes aegypti is incorporated into the framework of a stochastic population dynamics model able to handle seasonal and total extinction
as well as endemic situations. The model incorporates explicitly the dependence with temperature. The ecological parameters
of the model are tuned to the present populations of Aedes aegypti in Buenos Aires city, which is at the border of the present day geographical distribution in South America. Temperature thresholds
for the mosquito survival are computed as a function of average yearly temperature and seasonal variation as well as breeding
site availability. The stochastic analysis suggests that the southern limit of Aedes aegypti distribution in South America is close to the 15^∘C average yearly isotherm, which accounts for the historical and current
distribution better than the traditional criterion of the winter (July) 10°C isotherm. 相似文献
4.
Dr. R. E. Duhrkopf S. S. Y. Young 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1979,55(6):263-268
Summary Replicated divergent selection based upon the time taken to recover from the larval alarm reaction in the mosquito Aedes aegypti resulted in lines which recovered faster and slower than the control lines. Estimates of the realized heritability were consistent, ranging from 0.21 to 0.24 in the fast replicates and 0.19 to 0.20 in the slow replicates. After 11 generations of selection an apparent change in the fitness was examined using an application of the path analysis. The relevance of the findings to natural selection is also discussed. 相似文献
5.
Dengue is a human disease transmitted by the mosquito Aedes aegypti. For this reason geographical regions infested by this mosquito species are under the risk of dengue outbreaks. In this work, we propose a mathematical model to study the spatial dissemination of dengue using a system of partial differential reaction-diffusion equations. With respect to the human and mosquito populations, we take into account their respective subclasses of infected and uninfected individuals. The dynamics of the mosquito population considers only two subpopulations: the winged form (mature female mosquitoes), and an aquatic population (comprising eggs, larvae and pupae). We disregard the long-distance movement by transportation facilities, for which reason the diffusion is considered restricted only to the winged form. The human population is considered homogeneously distributed in space, in order to describe localized dengue dissemination during a short period of epidemics. The cross-infection is modeled by the law of mass action. A threshold value as a function of the model's parameters is obtained, which determines the rate of dengue dissemination and the risk of dengue outbreaks. Assuming that an area was previously colonized by the mosquitoes, the rate of disease dissemination is determined as a function of the model's parameters. This rate of dissemination of dengue disease is determined by applying the traveling wave solutions to the corresponding system of partial differential equations. 相似文献
6.
Simulating phenological characteristics of two dominant grass species in a semi-arid steppe ecosystem 总被引:3,自引:0,他引:3
Wenping Yuan Guangsheng Zhou Yuhui Wang Xi Han Yingshun Wang 《Ecological Research》2007,22(5):784-791
Vegetation phenology has a strong effect on terrestrial carbon cycles, local weather, and global radiation partitioning between
sensible and latent heat fluxes. Based on phenological data that were collected from a typical steppe ecosystem at Xilingol
Grazing and Meteorological Station from 1985 to 2003, we studied the phenological characteristics of Leymus chinensis and Stipa krylovii. We found that the dates for budburst of L. chinensis and S. krylovii were delayed with increasing temperature during winter and spring seasons; these results differed from existing research
in which earlier spring events were attributed to the changes in increasing air temperature in winter and spring. The results
also suggested that water availability was an important controlling factor for phenology in addition to temperature in grassland
plants. The classical cumulative temperature model simulated the phenology well in wet years, but not in the beginning of
growing season in all years from 1985 to 2003. The disparity between the simulation and the observation appeared to be related
to soil water. Based on our research findings, a water-heat-based phenological model was developed for simulating the beginning
of growing season for these two grass species. The simulated results of the new model showed a significant correlation with
the observation of beginning date of the growing season, and both mean values of the absolute error were less than 6 days. 相似文献
7.
Anton S van Loon JJ Meijerink J Smid HM Takken W Rospars JP 《Arthropod Structure & Development》2003,32(4):319-327
In insects, olfactory receptor neurons (ORNs) are located in cuticular sensilla, that are present on the antennae and on the maxillary palps. Their axons project into spherical neuropil, the glomeruli, which are characteristic structures in the primary olfactory center throughout the animal kingdom. ORNs in insects often respond specifically to single odor compounds. The projection patterns of these neurons within the primary olfactory center, the antennal lobe, are, however, largely unknown.We developed a method to stain central projections of intact receptor neurons known to respond to host odor compounds in the malaria mosquito, Anopheles gambiae. Terminal arborizations from ORNs from antennal sensilla had only a few branches apparently restricted to a single glomerulus. Axonal arborizations of the different neurons originating from the same sensillum did not overlap.ORNs originating from maxillary palp sensilla all projected into a dorso-medial area in both the ipsi- and contralateral antennal lobe, which received in no case axon terminals from antennal receptor neurons. Staining of maxillary palp receptor neurons in a second mosquito species (Aedes aegypti) revealed unilateral arborizations in an area at a similar position as in An. gambiae. 相似文献
8.
María C. Tranchida Arnaldo Maci Francisco Brusa María V. Micieli Juan J. García 《Biological Control》2009,49(3):270-276
We conducted a field survey for flatworms to select species as potential biological control agents against Aedes aegypti and Culex pipiens (Diptera, Culicidae) breeding in artificial containers. Laboratory experiments were performed to determine the daily predation rate, differential predation on each mosquito larval instar, selective predation on either A. aegypti or C. pipiens, and predator tolerance to water from artificial containers. Girardia anceps (Tricladida, Paludicola, Dugesiidae), Mesostoma ehrenbergii and Bothromesostoma cf. evelinae (Rhabdocoela, Typhloplanoida, Typhloplanidae) were found in temporary puddles and permanent pools. In the laboratory, they killed between 52% and 100% of immature mosquitoes coexisting in the same habitat. No preference of flatworms for mosquito preys was detected. Predation rate was related to predator size and instar of preys. Girardia anceps and B. evelinae survived after a dry period and when re-flooding occurred, they laid eggs. Tolerance to water from artificial containers was highest in G. anceps and this species could be a suitable predator to reduce mosquito populations from artificial containers using an inoculative approach. 相似文献
9.
Modeling a single season of Aedes albopictus populations based on host‐seeking data in response to temperature and precipitation in eastern Tennessee
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In the southern Appalachia of the U.S., Aedes mosquitoes maintain and transmit La Crosse virus (LACV) which causes La Crosse encephalitis, a neuroinvasive disease of children. In response to mosquito outbreaks, communities organize prevention, detection, and response measures that are dependent on local characteristics of the mosquito population and the community. Knowing Ae. albopictus is an accessory vector of LACV and a nuisance biter, our objective was to build a system of ordinary differential equations to model dynamics in a single season using our data and readily available environmental variables that can reflect the abundance and activity of Ae. albopictus. Consequently, we built an Ae. albopictus single‐season mathematical model for eastern Tennessee to fit our 2013 mosquito collection data in order to understand the population fluctuations. We included precipitation, temperature, and rate of change of temperature in the model because Aedes mosquitoes oviposit desiccant tolerant eggs with peak activity occurring over 26° C and those data are readily available and used frequently as forecast predictors. Our ordinary differential equation model accurately fits the data and facilitates predictions and better understanding of Ae. albopictus populations in southern Appalachia. 相似文献
10.
Crescenzio Francesco Minervini Luigi Viggiano Ruggiero Caizzi Ren Massimiliano Marsano 《Gene》2009,440(1-2):42-49
We have detected seventy-six novel LTR retrotransposons in the genome of the mosquito Aedes aegypti by a genome wide analysis using the LTR_STRUC program. We have performed a phylogenetic classification of these novel elements and a distribution analysis in the genome of A. aegypti. These mobile elements belong either to the Ty3/gypsy or to the Bel family of retrotransposons and were not annotated in the mosquito LTR retrotransposon database (TEfam). We have found that 1.8% of the genome is occupied by these newly detected retrotransposons that are distributed predominantly in intergenic genomic sequences and introns. The potential role of retrotransposon insertions linked to host genes is described and discussed. We show that a retrotransposon family belonging to the Osvaldo lineage has peculiar structural features, and its presence is likely to be restricted to the A. aegypti and to the Culex pipiens quinquefasciatus genomes. Furthermore we show that the ninja-like group of elements lacks the Primer Binding Site (PBS) sequence necessary for the replication of retrotransposons. These results integrate the knowledge on the complicate genomic structure of an important disease vector. 相似文献
11.
Estimation of the number of full sibling families at an oviposition site using RAPD-PCR markers: applications to the mosquito Aedes aegypti 总被引:1,自引:0,他引:1
B. L. Apostol W. C. Black IV B. R. Miller P. Reiter B. J. Beaty 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1993,86(8):991-1000
There are many species in which groups of individuals encountered in the field are known to consist of mixtures of full-sibling families. We describe a statistical technique, based on the use of random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) markers, that allows for the estimation of the number of families contained in these groups. We test the technique on full-sibling families of the mosquito Aedes aegypti, a species that distributes its eggs among several locations. Mixtures of 10 families with 15 individuals per family were analyzed using 40 RAPD-PCR loci amplified by 5 primers. Our analysis accurately estimated the number of families. The technique was accurate when the number of families was small or when family sizes were small and variable. 相似文献
12.
The nutrient base of aquatic tree-hole communities is derived from leaf litter, benthic detritus, and water flowing down the
tree trunk (stemflow water). Previous studies in eastern North America with the mosquito, Aedes triseriatus, have identified leaf litter as a major and stemflow water as a minor source of mosquito nutrition, but did not consider
the role of the benthic detritus or how the aggregate or relative contribution of these sources of mosquito nutrition changed
during the year. We use the leaf litter, benthic detritus, and stemflow water from tree holes in western Oregon (USA) to determine
how these substrates affect mass at metamorphosis, biomass yield, and fitness (cohort replacement rate; R
0) of the mosquito, Aedes sierrensis, through both natural and simulated winters, the normal growing season for larvae in tree holes. We found that fresh leaf
litter constitutes the major determinant of mosquito fitness by a factor of >15:1 over any other substrate taken directly
from tree holes in nature. The other substrates, including the benthic detritus, individually make only a meager contribution
to mosquito fitness but, when added to the leaf litter, can sustain yield and improve fitness at high, limiting larval densities.
Nutritional quality of tree-hole substrates declines by >90% from early (fall) to late (spring) in the larval growing season.
At both times of year, the coarse or fine detritus provide minor resources, and stemflow water provides no detectable contribution
to mosquito nutrition. The resources in the litter are not transported during the year to the benthic detritus; rather, these
resources are either exploited by mosquitoes when they first become available, or they deteriorate and become progressively
more unavailable to them. Growth and development of A. sierrensis feeding on dried and reconstituted tree-hole contents during a 6-month simulated winter in the laboratory showed: (1) the
same relative contributions of leaf litter, benthic detritus, and stemflow water to mosquito nutrition, (2) that the winter
deterioration of substrate quality is a direct consequence of microbial decomposition, and (3) that pre-emptive competition
from pre-existing A. sierrensis greatly increases substrate deterioration. We conclude that the progressive winter deterioration of larval resources in combination
with the dry summers of western North America are the most likely environmental factors that limit species diversity in tree
holes and that have selected for early recruitment (autumnal hatching) of A. sierrensis and for its univoltine life cycle from Mexico to Canada.
Received: 28 December 1999 / Accepted: 10 April 2000 相似文献
13.
Dawn L. Geiser Meng-Chieh Shen Jonathan J. Mayo Joy J. Winzerling 《Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology》2009,152(4):352-363
Ferritin is a multimer of 24 subunits of heavy and light chains. In mammals, iron taken into cells is stored in ferritin or incorporated into iron-containing proteins. Very little ferritin is found circulating in mammalian serum; most is retained in the cytoplasm. Female mosquitoes, such as Aedes aegypti (yellow fever mosquito, Diptera), require a blood meal for oogenesis. Mosquitoes receive a potentially toxic level of iron in the blood meal which must be processed and stored. We demonstrate by 59Fe pulse-chase experiments that cultured A. aegypti larval CCL-125 cells take up iron from culture media and store it in ferritin found mainly in the membrane fraction and secrete iron-loaded ferritin. We observe that in these larval cells ferritin co-localizes with ceramide-containing membranes in the absence of iron. With iron treatment, ferritin is found associated with ceramide-containing membranes as well as in cytoplasmic non-ceramide vesicles. Treatment of CCL-125 cells with iron and CI-976, an inhibitor of lysophospholipid acyl transferases, disrupts ferritin secretion with a concomitant decrease in cell viability. Interfering with ferritin secretion may limit the ability of mosquitoes to adjust to the high iron load of the blood meal and decrease iron delivery to the ovaries reducing egg numbers. 相似文献
14.
A non-autonomous dynamical system, in which the seasonal variation of a mosquito vector population is modeled, is proposed
to investigate dengue overwintering. A time-dependent threshold, R(t), is deduced such that when its yearly average, denoted by , is less than 1, the disease does not invade the populations and when is greater than 1 it does. By not invading the population we mean that the number of infected individuals always decrease
in subsequent seasons of transmission. Using the same threshold, all the qualitative features of the resulting epidemic can
be understood. Our model suggests that trans-ovarial infection in the mosquitoes facilitates dengue overwintering. We also
explain the delay between the peak in the mosquitoes population and the peak in dengue cases.
An erratum to this article can be found at 相似文献
15.
Bacillus thuringiensis var. israelensis, a gram positive, spore-forming bacillus, produces parasporal crystal protein during sporulation, which is toxic in the mosquito larvae gut. An efficient downstream processing method for separating the spore crystal complex (SCC) from the fermented broth of B. thuringiensis var. israelensis is required to achieve maximum mosquitocidal activity. The different downstream processing methods, viz., tangential flow ultra-filtration, continuous centrifugation and acid precipitation were compared for their efficiency in separating SCC from broth obtained from a pilot-scale fermentor (100 l capacity). Among the three downstream processing methods, tangential flow ultra-filtration yielded the maximum amount of biomass (53.3 g/l), maximum number of spores (2.30 × 1018 CFU/ml) and highest level of larvicidal activity (LC50 28 nl/ml) against Aedes aegypti Bora-Bora strain followed by continuous centrifugation and acid precipitation methods. 相似文献
16.
Seed dispersal is a critical stage in the life history of plants. It determines the initial pattern of juvenile distribution,
and can influence community dynamics and the evolutionary trajectories of individual species. Vertebrate frugivores are the
primary vector of seed dispersal in tropical forests; however, most studies of seed dispersal focus on birds, bats and monkeys.
Nevertheless, South America harbors at least 200 species of frugivorous fishes, which move into temporarily flooded habitats
during lengthy flood seasons and consume fruits that fall into the water; and yet, we know remarkably little about the quality
of seed dispersal they effect. We investigated the seed dispersal activities of two species of large-bodied, commercially
important fishes (Colossoma macropomum and Piaractus brachypomus, Characidae) over 3 years in Pacaya-Samiria National Reserve (Peru). We assessed the diet of these fishes during the flood
season, conducted germination trials with seeds collected from digestive tracts, and quantified fruit availability. In the
laboratory, we fed fruits to captive Colossoma, quantified the proportion of seeds defecated by adult and juvenile fish, and used these seeds in additional germination
experiments. Our results indicate that Colossoma and Piaractus disperse large quantities of seeds from up to 35% of the trees and lianas that fruit during the flood season. Additionally,
these seeds can germinate after floodwaters recede. Overexploitation has reduced the abundance of our focal fish species,
as well as changed the age structure of populations. Moreover, older fish are more effective seed dispersers than smaller,
juvenile fish. Overfishing, therefore, likely selects for the poorest seed dispersers, thus disrupting an ancient interaction
between seeds and their dispersal agents.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
17.
Hydrochory,seed banks,and regeneration dynamics along the landscape boundaries of a forested wetland 总被引:4,自引:0,他引:4
Following the environmental sieve concept, the setting in which the recruitment of Taxodium distichum occurs in, becomes increasingly restrictive from the seed to seedling stage in an impounded forested wetland. Although a wide elevational band of dispersing seed moves across the boundary of a swamp-field in the water sheet, the zone of germination is relegated to that portion of the forested wetland that draws down during the growing season. Seedling recruitment is further restricted to the uppermost zone of the winter water sheet. These patterns are likely applicable to other species of dominant swamp species, e.g., Cephalanthus occidentalis crossed the boundary of a forested wetland and abandonded field in winter flooding (November–December and November–March, respectively) in Buttonland Swamp. The elevation of the boundary was 101.3 m NGVD. While the seeds of at least 40 swamp species were dispersed across the boundary, few viable seeds were dispersed after the winter season. Kriged maps showed seeds of T. distichum and C. occidentalis dispersed in patches in the water depending on the position of the water sheet. Most species of both water- and gravity-dispersed species had a localized pattern of seed distribution (either spherical or exponential) and this indicated that seeds may not be dispersed for great distances in the swamp. Water-dispersed T. distichum and C. occidentalis had larger dispersal ranges (A
0=225 and 195 m, respectively) than Bidens frondosa and B. discoidea (A
0=14 and 16 m, respectively). Seed dispersal varied with season depending on the availability of seeds. In Buttonland Swamp, viable seeds typically were dispersed for T. distichum in November–June, and for C. occidentalis in November-July. Low water occurred in August 1993 and high in February 1994 (99.8 and 101.6 m NGVD, respectively). The seed banks along the landscape boundary varied in species composition according to elevation (r
2 = 0.996). While the similarity of species richness between water-dispersed seeds and the seed bank at elevations that flooded (during June 1993 through May 1995) was high (10–17%), it was low between water-dispersed seeds and the seed bank at elevations that did not flood (5%). T. distichum seeds had a short germination window in that seeds germinated within a year following their production in zones that were flooded in the winter followed by drawdown during the next growing season. After 1 year, less than 5% of the T. distichum seeds remained viable on the surface of the soil. Germination of T. distichum was confined to specific elevations (above 99.3 but below 101.6 m NGVD) during this study with 4.1% of the seedlings surviving for more than 2 years at a mean of 101.4 m NGVD. All seedlings below this elevation died. To maximize natural regeneration along the boundaries of swamps in abandoned farm fields targeted for restoration, this study suggests a flood pulse regime consisting of high water in the winter to maximize dispersal of live seeds followed by low water in the summer to facilitate seed germination and seedling recruitment. Hydrologic restoration could assist in the natural recovery of damaged wetlands if a seed source exists nearby. 相似文献
18.
According to most studies on seed dispersal in tropical forests, mammals and birds are considered the main dispersal agents
and the role played by other animal groups remains poorly explored. We investigate qualitative and quantitative components
of the role played by the tortoise Chelonoidis denticulata in seed dispersal in southeastern Amazon, and the influence of seasonal variation in tortoise movement patterns on resulting
seed shadows. Seed shadows produced by this tortoise were estimated by combining information on seed passage times through
their digestive tract, which varied from 3 to 17 days, with a robust dataset on movements obtained from 18 adult C. denticulata monitored with radio transmitters and spoon-and-line tracking devices. A total of 4,206 seeds were found in 94 collected
feces, belonging to 50 seed morphotypes of, at least, 25 plant genera. Very low rates of damage to the external structure
of the ingested seeds were observed. Additionally, results of germination trials suggested that passage of seeds through C. denticulata’s digestive tract does not seem to negatively affect seed germination. The estimated seed shadows are likely to contribute
significantly to the dispersal of seeds away from parent plants. During the dry season seeds were dispersed, on average, 174.1 m
away from the location of fruit ingestion; during the rainy season, this mean dispersal distance increased to 276.7 m. Our
results suggest that C. denticulata plays an important role in seed dispersal in Amazonian forests and highlight the influence of seasonal changes in movements
on the resulting seed shadows. 相似文献
19.
20.
The left (5) inverted terminal repeat (ITR) of the Mos1 mariner transposable element was altered by site-directed mutagenesis so that it exactly matched the nucleotide sequence of the right (3) ITR. The effects on the transposition frequency resulting from the use of two 3 ITRs, as well as those caused by the deletion of internal portions of the Mos1 element, were evaluated using plasmid-based transposition assays in Escherichia coli and Aedes aegypti. Donor constructs that utilized two 3 ITRs transposed with greater frequency in E. coli than did donor constructs with the wild-type ITR configuration. The lack of all but 10 bp of the internal sequence of Mos1 did not significantly affect the transposition frequency of a wild-type ITR donor. However, the lack of these internal sequences in a donor construct that utilized two 3 ITRs resulted in a further increase in transposition frequency. Conversely, the use of a donor construct with two 3 ITRs did not result in a significant increase in transposition in Ae. aegypti. Furthermore, deletion of a large portion of the internal Mos1 sequence resulted in the loss of transposition activity in the mosquito. The results of this study indicate the possible presence of a negative regulator of transposition located within the internal sequence, and suggest that the putative negative regulatory element may act to inhibit binding of the transposase to the left ITR. The results also indicate that host factors which are absent in E. coli, influence Mos1 transposition in Ae. aegypti.Communicated by G. P. Georgiev 相似文献